SARMs and method of use thereof

ABSTRACT

This invention is directed to substituted acylanilide compounds and uses thereof in treating muscular dystrophies such as Duchenne muscular dystrophy and Becker muscular dystrophy and in improving or preserving lung function and cardiac function in a subject suffering from Duchenne muscular dystrophy.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a Continuation Application of U.S. patentapplication Ser. No. 15/190,732, filed Jun. 23, 2016, which is aContinuation-in-Part application of U.S. patent application Ser. No.15/153,690, filed May 12, 2016, which is a Continuation-in-Partapplication of U.S. patent application Ser. No. 14/963,054, filed Dec.8, 2015, which is a Continuation-in-Part application of U.S. patentapplication Ser. No. 14/168,993, filed Jan. 30, 2014, now U.S. Pat. No.9,278,914, which is a Continuation Application of U.S. patentapplication Ser. No. 13/627,900, filed Sep. 26, 2012, now U.S. Pat. No.8,669,286, which is a Continuation Application of U.S. patentapplication Ser. No. 12/730,094, filed Mar. 23, 2010, now U.S. Pat. No.8,309,603; and U.S. patent application Ser. No. 14/168,993, now U.S.Pat. No. 9,278,914, which is a Continuation in Part application of U.S.patent application Ser. No. 13/868,768, filed Apr. 23, 2013, now U.S.Pat. No. 8,846,756, which is a Continuation application from U.S. patentapplication Ser. No. 13/302,988, filed Nov. 22, 2011, now U.S. Pat. No.8,426,465, which is a Divisional application from U.S. patentapplication Ser. No. 11/892,595, filed Aug. 24, 2007, now U.S. Pat. No.8,080,682, which claims the benefit of U.S. Provisional Application Ser.No. 60/839,665, filed Aug. 24, 2006, and U.S. Provisional ApplicationSer. No. 60/907,749, filed Apr. 16, 2007; all of which are herebyincorporated by reference in their entirety.

GOVERNMENT INTEREST STATEMENT

This invention was made in whole or in part with government supportunder grant number R01 DK598006, awarded by the National Institute ofHealth; under grant number R29 CA068096 awarded by the National CancerInstitute, National Institute of Health; under grant number R15 HD35329,awarded by the National Institute of Child Health and Human Development,National Institute of Health and under grant number R01 DK59800, awardedby the National Institute of Health. The government may have certainrights in the invention.

FIELD OF THE INVENTION

This invention provides substituted acylanilide compounds and usesthereof in treating muscular dystrophies such as Duchenne musculardystrophy and Becker muscular dystrophy and in improving or preservinglung function and cardiac function in a subject suffering from musculardystrophies.

BACKGROUND OF THE INVENTION

Muscle wasting refers to the progressive loss of muscle mass and/or tothe progressive weakening and degeneration of muscles, including theskeletal or voluntary muscles, which control movement, cardiac muscles,which control the heart (cardiomyopathies), and smooth muscles. Chronicmuscle wasting is a chronic condition (i.e. persisting over a longperiod of time) characterized by progressive loss of muscle mass, andweakening and degeneration of muscle.

The loss of muscle mass that occurs during muscle wasting can becharacterized by muscle protein degradation by catabolism. Proteincatabolism occurs because of an unusually high rate of proteindegradation, an unusually low rate of protein synthesis, or acombination of both. Muscle protein catabolism, whether caused by a highdegree of protein degradation or a low degree of protein synthesis,leads to a decrease in muscle mass and to muscle wasting.

Muscle wasting is associated with chronic, neurological, genetic orinfectious pathologies, diseases, illnesses or conditions. These includemuscular dystrophies such as Duchenne muscular dystrophy, Beckermuscular dystrophy, limb-girdle disease, and myotonic dystrophy; muscleatrophies such as post-polio muscle atrophy (PPMA); cachexias such ascardiac cachexia, AIDS cachexia and cancer cachexia; and malnutrition,leprosy, diabetes, renal disease, chronic obstructive pulmonary disease(COPD), cancer, end stage renal failure, sarcopenia, emphysema,osteomalacia, HIV infection, AIDS, and cardiomyopathy.

In addition, other circumstances and conditions are linked to and cancause muscle wasting. These include chronic lower back pain, advancedage, central nervous system (CNS) injury, peripheral nerve injury,spinal cord injury, chemical injury, central nervous system (CNS)damage, peripheral nerve damage, spinal cord damage, chemical damage,burns, disuse deconditioning that occurs when a limb is immobilized,long term hospitalization due to illness or injury, and alcoholism.

An intact androgen receptor (AR) signaling pathway is crucial forappropriate development of skeletal muscles. Furthermore, an intactAR-signaling pathway increases lean muscle mass, muscle strength andmuscle protein synthesis.

Muscle wasting, if left unabated, can have dire health consequences. Forexample, the changes that occur during muscle wasting can lead to aweakened physical state that is detrimental to an individual's health,resulting in increased susceptibility to bone fracture and poor physicalperformance status. In addition, muscle wasting is a strong predictor ofmorbidity and mortality in patients suffering from cachexia and AIDS.

Duchenne muscular dystrophy is the most common of nine musculardystrophies and occurs in 1/3500 to 1/5000 males around the world.Duchenne muscular dystrophy patients experience difficulty with walkingat 3-5 years of age, progressive worsening of symptoms, and death in theteens to 3^(rd) decade. Discovered in the 1860's, little was known aboutthe pathogenesis of Duchenne muscular dystrophy until 1986 when the geneunderlying this X-linked autosomal recessive disease was cloned andcharacterized. The gene was named dystrophin (DMD) and found to be partof a sarcolemma (i.e. myocte plasma membrane) protein complex(dystrophin-glycoprotein complex) which connects the myofibril (musclecell) cytoskeleton to the extracellular matrix, thereby protecting themuscle cell membrane from physical trauma during muscle exertion andexercise. Duchenne muscular dystrophy is predominantly a disease inmales and is associated with a variety of mutations of the DMD genewhich leads to a wide variation of disease severities. Sarcolemmafragility produces progressive calcium permeability, proteaseactivation, oxidative stress, and inflammation which causes progressivereplacement of muscle cells by fibrous tissue and/or conversion to fat.Gross pathology includes weakness and degeneration of skeletal andvoluntary muscle which is exacerbated by high impact exercise, musclecontractures that worsen mobility if not corrected, and scoliosis.Although braces and walkers provide some protection, declines inphysical function result in loss of ambulation during childhood leadingto wheelchair confinement, and eventually impaired cardiac(cardiomyopathy) or respiratory (diaphragm fibrosis) function leads todeath. Average life expectancy has improved (and rare cases of menliving into their 4^(th) or 5^(th) decade) as a result of betterrespiratory (glucocorticoids) and cardiac (ACE inhibitors, angiotensinreceptor blockers, and beta-blockers) supportive care but nodisease-modifying therapeutics exist. Anabolics (steroidal androgens,IGF-I, etc.) to slow the rate of physical function decline have beenproposed and were shown to provide some benefit in small clinicaltrials, but no nonsteroidal or tissue-selective androgen receptormodulator (SARM) has entered clinic testing for Duchenne musculardystrophy. The loss of gene function etiology has attracted greatinterest toward gene therapy approaches to treat the disease; however,such treatments have not completely reversed the phenotype and sufferfrom difficulties inherent in nucleotide polymer based therapeuticswhich are exacerbated by the large and complex nature of the dystrophingene. The above suggests that other therapeutic targets are urgentlyneeded. Consequently, there is increasing interest in further improvingthe quality of life and length of life via symptom directed supportivecare. Aryl propanamide SARMs have been shown to increase global anabolictone in multiple clinical trials through increases in muscle mass (leanbody mass by DEXA) and physical function (e.g., leg press, gripstrength, stair climb power) suggesting that they may have therapeuticeffects on dystrophic skeletal and specifically diaphragm muscle,cardiac, and smooth muscle, or may delay onset or improve symptoms ofloss of mobility/autonomy, cardiomyopathy, or respiratory insufficiencyin Duchenne muscular dystrophy or Becker muscular dystrophy and othermuscular dystrophy patients.

Becker muscular dystrophy is a rarer and milder variation of Duchennemuscular dystrophy caused by DMD mutants that do not completely abrogatedystrophin glycoprotein complex function in males or more commonly it isobserved in some female carriers (Duchenne muscular dystrophy is oftenasymptomatic in females). Becker muscular dystrophy has a phenotype withless functional impairment and longer life expectancy, but clinicalcardiomyopathies and respiratory insufficiencies must be closelymonitored.

Interest in drug design for Duchenne muscular dystrophy was hampered bythe lack of good models of this disease, however several in vivo diseasemodels now exist. These include the dystrophin gene deletion in mice(mdx mice; denoted by DMD (−/−)) which presents a phenotyperepresentative of the early stages of the disease in humans however, isnot progressive in symptomology and much less severe in the later stagesof the disease. Double-knockout (knock-down) mice lacking dystrophin(DMD) and utrophin (UTRN, a protein that can partially compensate forlack of dystrophin) (i.e., DMD (−/−) UTRN (−/−)) present a phenotypemore representative of the natural history of Duchenne musculardystrophy in humans including progressive worsening of symptoms, loss ofambulation at ˜12 weeks, and early death by ˜20 weeks. [A severephenotype can also be derived from the mdx model by forced treadmillrunning.] Golden retriever muscular dystrophy is another disease modelthat matches the human phenotype in some ways but suffers from a highlevel of interindividual variation even among littermates, complicatingthe interpretation of results. Although the pathogenesis of othermuscular dystrophies is not to the same as Duchenne and Becker musculardystrophies, the phenotypes therein suggest that activity in the mdx anddouble knockout models may be indicative of therapeutic efficacies inthose disease states as well.

While there are many treatments and therapies for these conditions invarious phases of testing, none are ideal. Since the androgen receptor(AR) signaling pathway has been shown to increase lean muscle mass,muscle strength and muscle protein synthesis, and since hypogonadismaccompanies these conditions, molecules targeting the AR signalingpathway may be useful in treating these diseases and/or conditions, andmay be complementarity to other disease-modifying or symptom-directedtherapies for Duchenne muscular dystrophy.

SUMMARY OF THE INVENTION

In one embodiment, this invention provides: (i) a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof; (ii) a method of increasing the physical function of asubject suffering from Duchenne muscular dystrophy; (iii) a method ofincreasing the quality of life of a subject suffering from Duchennemuscular dystrophy; (iv) a method of increasing the survival of asubject suffering from Duchenne muscular dystrophy; (v) a method oftreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of cardiomyopathy in a subjectsuffering from Duchenne muscular dystrophy; (vi) a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of respiratory failure in a subject sufferingfrom Duchenne muscular dystrophy; (vii) a method of treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Becker muscular dystrophy; (viii) a method oftreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of myotonic dystrophy, limb-girdlemuscular dystrophy, facioscapulhumeral muscular dystrophy, congenitalmuscular dystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy; (ix) a method ofimproving or preserving lung function of a subject suffering fromDuchenne muscular dystrophy; (x) a method of improving or preservingcardiac function of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis wherein said SARM compound is represented by the structureof formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, the present invention further increases the physicalfunction of said subject.

In one embodiment, the present invention further increases the qualityof life of said subject.

In one embodiment, the present invention increases the survival of saidsubject.

In one embodiment, the present invention delays onset or improvessymptoms of cardiomyopathy and/or respiratory function.

BRIEF DESCRIPTION OF THE DRAWINGS

The present invention will be understood and appreciated more fully fromthe following detailed description taken in conjunction with theappended drawings in which:

FIGS. 1A-1L: Synthetic schemes for the preparation of compound offormula II. FIG. 1A is a synthetic scheme for the preparation of an (S)enantiomer of a compound of formula II (S-II). FIG. 1B is a syntheticscheme for the preparation of an (R) enantiomer of a compound of formulaII (R-II). FIG. 1C is a synthetic scheme for the preparation of an (S)enantiomer of a compound of formula II (S-II) including an oxiraneintermediate. FIG. 1D is a synthetic scheme for the preparation of an(R) enantiomer of a compound of formula II (R-II) including an oxiraneintermediate. FIG. 1E is a synthetic scheme for the preparation of an(S) enantiomer of a compound of formula II (S-II) involving B-ringaddition prior to A-ring addition. FIG. 1F is a synthetic scheme for thepreparation of an (R) enantiomer of a compound of formula II (R-II)involving B-ring addition prior to A-ring addition. FIG. 1G is asynthetic scheme for the preparation of an (S) enantiomer of a compoundof formula II (S-II) using 2-tribromomethyl-[1,3]dioxolan-4-oneintermediate and involving B-ring addition prior to A-ring addition.FIG. 1H is a synthetic scheme for the preparation of an (R) enantiomerof a compound of formula II (R-II) using2-tribromomethyl-[1,3]dioxolan-4-one intermediate and involving B-ringaddition prior to A-ring addition. FIG. 1I is a synthetic scheme forpreparation of a racemic mixture of a compound of formula II, involvingoxazolidinedione intermediate and B ring addition prior to A ring. FIG.1J is a synthetic scheme for preparation of a racemic mixture of acompound of formula II, involving an oxirane intermediate and A ringaddition prior to B ring. FIG. 1K is a synthetic scheme for preparationof a large scale of an (S) enantiomer of a compound of formula II(S-II). FIG. 1L is a synthetic scheme for preparation of a large scaleof an (S) enantiomer of a compound of formula II (S-II), including anoxirane intermediate.

FIG. 2: Anabolic and androgenic pharmacology of compound of formula S-IIin castrated rats (ORX).

FIG. 3: Levator ani weight effects in castrated rats for a panel ofcompounds.

FIG. 4: Prostate weight effects in castrated rats for a panel ofcompounds.

FIGS. 5A-5D: Effect of compound of formula S-II on the growthperformance and carcass composition of finishing pigs. FIG. 5A shows theincrease of average daily gain (ADG) over the course of the study. FIG.5B shows the decrease feed to gain ratio. FIG. 5C shows the increasedfat free lean gain per day. FIG. 5D shows an increase in ADG for days21-28.

FIG. 6: Depicts a synthetic scheme for the preparation of an (S)enantiomer of a compound of formula XXIII (S-XXIII).

FIG. 7: Pharmacology of a compound of formula S-XXIII in intact rats.Asterisks represent statistically significant differences between theweight of the organ in the indicated group and that observed in intactanimals treated with vehicle (P<0.05).

FIG. 8: Organ weights from castrated, compound of formulaS-XXIII-treated rats presented as a percentage of intact control. *P-value<0.05 versus intact controls.

FIG. 9: Organ weight maintenance dose-response curves for a compound offormula S-XXIII and seminal vesicles (closed squares) were obtained bynonlinear regression analysis using the sigmoid E_(max) model inWinNonlin®.

FIG. 10: Depicts a synthetic scheme for the preparation of an (S)enantiomer of a compound of formula XXIV (S-XXIV).

FIG. 11: Anabolic and androgenic activity of a compound of formulaS-XXIV in ORX rats.

FIG. 12: Depicts a synthetic scheme for the preparation of an (S)enantiomer of a compound of formula XXV (S-XXV).

FIG. 13: Anabolic and androgenic activity of a compound of formula S-XXVin ORX rats.

FIGS. 14A-14D show that compound of formula S-XXIII in the DMD singleknockout or mdx mouse model (DMD (−/−) UTRN (+/+)), e.g., increased bodyweight and lean mass. FIG. 14A shows the effects of S-XXIII on bodyweight in DMD (−/−) UTRN (+/+) mice. FIG. 14B shows the effects ofS-XXIII on fat mass in DMD (−/−) UTRN (+/+) mice. FIG. 14C shows theeffects of S-XXIII on lean mass in DMD (−/−) UTRN (+/+) mice. FIG. 14Dshows the effects of S-XXIII on grip strength in DMD (−/−) UTRN (+/+)mice.

FIGS. 15A-15C show that ‘SARMs’ (cumulatively S-XXIII, S-XXIV, and S-XXV(combined data for groups 2-4 in Example 16)) delayed the deteriorationof body weight, lean mass, and grip strength of DMD (−/−) UTRN (−/−)double knockout mice. FIG. 15A shows the effects of S-XXIII on bodyweight of DMD (−/−) UTRN (−/−) mice. FIG. 15B shows the effects ofS-XXIII on lean mass of DMD (−/−) UTRN (−/−) mice. FIG. 15C shows theeffects of S-XXIII on grip strength in DMD (−/−) UTRN (−/−) mice. N=6-9in each group.

FIGS. 16A and 16B show that compounds of formulas S-XXIII or S-XXV(combined data labeled as ‘SARM’) and S-XXIII, respectively, increasedthe survival by 50-70% in DMD (−/−) UTRN (−/−) double knockout mice.FIG. 16A shows cumulative data whereas FIG. 16B shows data from micefrom the same litter (each group of two bars represents one litter).Despite similar characteristics at birth, mice treated with ‘SARM’ orS-XXIII, respectively, exhibited increased survival than mice treatedwith vehicle.

FIGS. 17A-17C show magnetic resonance imaging (MRI) data that compoundof formula S-XXIII increased body weight (FIG. 17A), lean mass (FIG.17B), and grip strength (FIG. 17C) in DMD (−/−) UTRN (+/+) mice(separate experiment from mice in Example 16) which were furthercharacterized by echocardiography in FIGS. 18A-18D.

FIGS. 18A-18D show the effects of S-XXIII on cardiac function in DMD(−/−) UTRN (+/+) mice via echocardiography results. FIG. 18A shows theeffects of S-XXIII on ejection fraction (%). FIG. 18B shows the effectsof S-XXIII on fractional shortening (FS) (%). FIG. 18C shows the effectsof S-XXIII on AV peak velocity (aortic valve peak velocity) (mm/s). FIG.18D shows the effects of S-XXIII on AV peak pressure (mmHg).

FIGS. 19A and 19B depict the effect of S-XXIII on body weight of intactDMD (−/−) UTRN (+/+) mice (FIG. 19B) in comparison with castrated mice(FIG. 19A).

FIGS. 20A and 20B depict the effect of S-XXIII on lean mass of intactDMD (−/−) UTRN (+/+) mice (FIG. 20B) in comparison with castrated mice(FIG. 20A).

FIGS. 21A-21C depict that S-XXIII improved lung function in castratedDMD (−/−) mice. S-XXIII reduced breathing rate (i.e., respiratory rate(RR)) as measured as bpm (breaths per minute) (FIG. 21A); increasedarterial saturated oxygen levels (SPO2%) (FIG. 21B); and reduced heartrate (HR) measured as beats per minute (bpm) (FIG. 21C). Cumulatively,this data suggest that S-XXIII treatment improved the cardiovascular andpulmonary function of DMD (−/−) mice, and may prevent or reversecardiomyopathy or respiratory failure in patients with Duchenne musculardystrophy.

FIGS. 22A-22D depict that S-XXIII improved oxygen consumption andmobility in castrated DMD (−/−) mice as measured by the comprehensivelaboratory animal monitoring system (CLAMS). FIG. 22A: volume of oxygenconsumed (VO2) in dark (active) conditions is shown; FIG. 22B: VO2 inlight conditions is shown; FIG. 22C: total activity in dark (active)conditions is shown; and FIG. 22D: total activity in light conditions isshown. Increased volume of consumed oxygen (VO2) indicates increasedenergy expenditure, coupled to increased physical activity suggests thatDMD (−/−) mice treated with S-XXIII had improved pulmonary function andimproved physical function (i.e., less disability).

FIG. 23 depicts that S-XXIII restored oxygen consumption (VO2) incastrated DMD (−/−) mice as measured by the comprehensive laboratoryanimal monitoring system (CLAMS) collected over a 2-day time period.This data further suggests that S-XXIII may be able to reverse orprevent disability and/or improve the quality of life of Duchennemuscular dystrophy patients.

FIG. 24 depicts that treatment of castrated DMD (−/−) mice with S-XXIIIreduced the methacholine (MeCh)-induced airway resistance significantlycompared to vehicle-treated castrated DMD (−/−) mice (flexiVent® data).The data suggest that S-XXIII may be able to prevent respiratory failureand/or improve pulmonary function in patients with Duchenne musculardystrophy.

FIGS. 25A-25D depict that circulating androgens in intact mice improvemuscle mass and survival in double knockout [DMD(−/−) UTRN (−/−)] or dKOmice. 4-6 week old dKO mice were sham-operated (i.e., intact) orcastrated and measured weekly for body weight (FIG. 25A) and lean mass(FIG. 25B) by MRI and their survival monitored (FIG. 25C (table) andFIG. 25D (Kaplan-Meier curve of same data)). Values are represented asaverage±S.E. * significance at p<0.01.

FIGS. 26A-26I depict that selective androgen receptor modulators (SARMs)including S-XXIII increased muscle mass and strength in MDX mice. FIGS.26A and 26B: S-XXIII is a potent activator of androgen receptor (AR). ARtransactivation studies were performed in HEK-293 cells (FIG. 26A) orC2C12 cells (FIG. 26B) by transfecting 25 ng CMV-hAR, 0.25 μg GRE-LUC,and 10 ng CMV-renilla-LUC. At 24 h after transfection, cells weretreated with vehicle, R1881, or one of three SARMs S-XXV, S-XXIII, orS-XXIV (FIG. 26A), or S-XXIII or DHT (FIG. 26B). Cells were harvested 48h after transfection and luciferase assay was performed. FIG. 26C: SARMsincreased muscle mass, physical function, and body weight of wild-typemice. C57BL/6 male mice (6 weeks old; n=6/group) were castrated toremove circulating androgens and treated subcutaneously with vehicle or10 mg/kg/day S-XXIII. S-XXIII significantly (p<0.001) increased bodyweight and lean mass starting after 1 week and maintained significantactivity at the end of treatment (12 weeks) (FIG. 26C). Grip strength,which was used as a measure of physical function, was significantlyincreased by S-XXIII from week 4 of treatment (FIG. 26C) compared tovehicle-treated animals. FIGS. 26D-F: S-XXIII increases muscle mass andstrength in MDX mice. Male MDX mice (4-6 weeks of age; n=6) werecastrated to reduce the circulating androgens and treated with vehicleor 10 mg/kg/day s.c. of S-XXIII. Weekly body weight (FIG. 26D) and MRIto measure the muscle mass (FIG. 26E) were obtained. Grip strength (FIG.26F) was measured after 6 and 12 weeks (solid bars indicatevehicle-treated; hashed bars indicated S-XXIII-treated). Intact MDX micewere also treated and analyzed in the same fashion (FIG. 26G) torepresent older boys with Duchenne muscular dystrophy where androgenlevels are higher (dashed line is S-XXIII-treated; solid line isvehicle-treated). FIGS. 26H and 26I: Castrated MDX mice treated withvehicle or S-XXIII were sacrificed and gastrocnemius and soleus muscle(n=3/group) were isolated and stained with hematoxylin and eosin (H&E).The numbers of centrally nucleated cells were counted and represented inthe bar graph (FIG. 26H; solid bars indicate vehicle-treated; hashedbars indicated S-XXIII-treated). Arrows point to the nucleus (FIG. 26I).FIGS. 26J and 26K: S-XXIII reduces fibrosis, necrosis and inflammation.Gastrocnemius muscle (n=3/group) from vehicle- or S-XXIII-treatedcastrated MDX mice were fixed and stained to measure fibrosis (trichromestaining), necrosis, and inflammation. The intensity of staining wasscored between 0 and 3. The range is represented in the table (FIG.26J). Representative trichrome staining is shown in FIG. 26K. Values arerepresented as average±S.E. * significance at p<0.01-0.001. FIGS.26L-26N: Lung fibrosis in MDX mice is decreased by endogenous androgenand S-XXIII treatment. The extent of fibrosis was evaluated in Masontrichrome-stained lungs of the MDX mice. At the end of treatment, lungtissues were fixed and stained for collagen by trichrome staining. Thesections were scored between 1 and 3 based on the intensity of staining(FIG. 26L). Collagen (dark blue stain) staining, which is indicative offibrosis, was modest in sham-operated (i.e., intact) MDX mice (FIG. 26M)and MDX mice castrated and treated with S-XXIII (FIG. 26O), while thestaining was intense in MDX mice castrated and treated with vehicle(FIG. 26N).

FIGS. 27A-27G depict that S-XXIII increases the expression of genesimportant for muscle function. Castrated wild-type or MDX mice treatedwith vehicle or 10 mg/kg/day s.c. S-XXIII for 12 weeks were sacrificed,gastrocnemius muscle was isolated, RNA isolated, and expression of geneswas measured by RNA-sequencing. FIG. 27A and FIG. 27B: Genes thatsignificantly were up-regulated by S-XXIII, compared to vehicle, incastrated MDX mice are represented here. FIG. 27C-FIG. 27E: Genes thatare significantly regulated in gastrocnemius muscle of castrated MDXcompared to castrated wild-type mice are represented. FIG. 27F and FIG.27G: MiRNAs are differentially regulated in MDX mice and in MDX micetreated with S-XXIII. RNA (n=3/group) isolated from gastrocnemius asdescribed above for FIGS. 27A and 27B were used to measure genome-widemiRNA expression. MiRNAs that were differentially regulated in MDX micecompared to wild-type, but not reversed by S-XXIII are shown in FIG. 27Fand the miRNAs regulated in MDX that were reversed by S-XXIII in MDXmice are shown in FIG. 27G. Values are represented as average±S.E.

FIG. 28A-FIG. 28D depict that S-XXIII increases body weight, musclemass, physical function, and survival in dKO mice. FIG. 28A-FIG. 28C:dKO mice were castrated and treated with vehicle or 10 mg/kg/day s.c.S-XXIII. Weekly body weight (FIG. 28A), lean mass (FIG. 28B), and gripstrength (FIG. 28C) were measured. FIG. 28D: dKO mice were castrated andtreated with vehicle or 10 mg/kg/day s.c. S-XXIII. Animals weremonitored for survival (N=6-8 mice). Values are represented asaverage±S.E. * significance at p<0.05.

DETAILED DESCRIPTION OF THE PRESENT INVENTION

In the following detailed description, numerous specific details are setforth in order to provide a thorough understanding of the invention.However, it will be understood by those skilled in the art that thepresent invention may be practiced without these specific details. Inother instances, well-known methods, procedures, and components have notbeen described in detail so as not to obscure the present invention.

This invention provides, in one embodiment, feed composition for animalscomprising acylanilides characterized by the structure of formulasI-XXV. In one embodiment, the compound is a SARM. In one embodiment, thecompound and/or feed composition is useful in affecting the carcasscomposition, increasing the lean mass, reducing the fat mass of ananimal or reducing percent fat mass, increasing feed efficiency,increasing average daily gain (ADG), decreasing feed to gain ratio (F:G)of an animal, including a feedlot animal, a beef cattle or a finishinglivestock. In another embodiment, the compound and/or feed compositionis useful in increasing muscle growth of an animal, modulation of meatquality, or enhancing productive life of animals including feedlotanimals, beef cattle and finishing livestock.

In one embodiment, the compounds of this invention provide compounds,compositions and methods of treating a variety of conditions ordiseases, including, inter alia, oral testosterone replacement therapy,male contraception, maintaining sexual desire in women, osteoporosis,treating prostate cancer and/or imaging prostate cancer. In someembodiments, the compounds of this invention are nonsteroidal ligandsfor the AR and exhibit androgenic and/or anabolic activity. In someembodiments, the compounds are partial agonists or partial antagonistsin a tissue selective manner. In some embodiments, the compounds arefull agonists or full antagonists in a tissue selective manner, which insome embodiments, allows for tissue-selective androgenic and/or anaboliceffects. These agents may be active alone or in combination withprogestins or estrogens, or other agents, as herein described. In otherembodiments, the agents are agonists, antagonists, partial agonists orpartial antagonists.

In some embodiments, this invention provides compounds, which are usefulin androgen replacement therapy (ART), useful in: a) improving bodycomposition; b) increasing bone mineral density (BMD); c) increasingbone mass; d) increasing bone strength; e) improving bone function; f)decreasing fracture risk; g) increasing muscle strength; h) increasingmuscle function; i) improving exercise tolerance; j) enhancing libido;k) improving sexual performance; l) improving mood; and/or m) improvingcognition.

In some embodiments, this invention provides synthetic processes ofpreparation of the SARM compounds of this invention. In someembodiments, the invention provides compositions comprising theselective androgen receptor modulator compounds or use of the same forbinding an AR, modulating spermatogenesis, bone formation and/orresorption, treating muscle wasting or diseases associated with musclewasting, treating prostate cancer, and/or providing hormonal therapy forandrogen-dependent conditions.

In one embodiment, the present invention provides, a compound of formula(I):

wherein Q₂ is alkyl, F, Cl, Br, I, CF₃, CN, C(R)₃, Sn(R)₃, N(R)₂,NHCOCH₃, NHCOCF₃, NHCOR, NHCONHR, NHCOOR, OCONHR, CONHR, NHCSCH₃,NHCSCF₃, NHCSR, NHSO₂CH₃, NHSO₂R, OR, COR, OCOR, OSO₂R, SO₂R, or SR; andR is alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH₂F, CHF₂, CF₃,CF₂CF₃, aryl, phenyl, F, Cl, Br, I, alkenyl or OH;or its isomer, pharmaceutically acceptable salt, pharmaceutical product,N-oxide, hydrate or any combination thereof.

In one embodiment, the present invention provides, a compound of formulaS-II:

-   -   or its isomer, pharmaceutically acceptable salt, pharmaceutical        product, N-oxide, hydrate or any combination thereof.

In one embodiment, the present invention provides, a compoundrepresented by the structure of formula III:

wherein

-   -   X is a bond, O, CH₂, NH, Se, PR, NO or NR;    -   G is O or S;    -   T is OH, OR, —NHCOCH₃, or NHCOR;    -   R is alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH₂F, CHF₂,        CF₃, CF₂CF₃, aryl, phenyl, halogen, alkenyl or OH;    -   R₁ is CH₃, CH₂F, CHF₂, CF₃, CH₂CH₃, or CF₂CF₃;    -   R₂ is H, F, Cl, Br, I, CH₃, CF₃, OH, CN, NO₂, NHCOCH₃, NHCOCF₃,        NHCOR, alkyl, arylalkyl, OR, NH₂, NHR, N(R)₂, or SR;    -   R₃ is H, F, Cl, Br, I, CN, NO₂, COR, COOH, CONHR, CF₃, or        Sn(R)₃; or        -   R₃ together with the benzene ring to which it is attached            forms a fused ring system represented by the structure:

-   -   Z is NO₂, CN, Cl, F, Br, I, H, COR, COOH, or CONHR;    -   Y is CF₃, alkoxy, alkyl, hydroxyalkyl, alkylaldehyde, formyl, H,        F, Br, Cl, I, CN, or Sn(R)₃;    -   Q is H, alkyl, halogen, CF₃, CN, C(R)₃, Sn(R)₃, N(R)₂, NHCOCH₃,        NHCOCF₃, NHCOR, NHCONHR, NHCOOR, OCONHR, CONHR, NHCSCH₃,        NHCSCF₃, NHCSR, NHSO₂CH₃, NHSO₂R, OH, OR, COR, OCOR, OSO₂R,        SO₂R, or SR; or Q together with the benzene ring to which it is        attached is a fused ring system represented by structure A, B or        C:

-   -   -   n is an integer of 1-4; and        -   m is an integer of 1-3;

    -   or an isomer, pharmaceutically acceptable salt, pharmaceutical        product, N-oxide, hydrate thereof or any combination thereof.

In one embodiment, the present invention provides, a feed compositionfor an animal comprising a compound of formula IIIA:

whereinZ is NO₂, CN, Cl, F, Br, I, H, COR, COOH, or CONHR;Y is CF₃, alkoxy, alkyl, hydroxyalkyl, alkylaldehyde, formyl, H, F, Br,Cl, I, CN, or Sn(R)₃;R is alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH₂F, CHF₂, CF₃,CF₂CF₃, aryl, phenyl, halogen, alkenyl or OH;R₂ is H, F, Cl, Br, I, CH₃, CF₃, OH, CN, NO₂, NHCOCH₃, NHCOCF₃, NHCOR,alkyl, arylalkyl, OR, NH₂, NHR, N(R)₂, or SR;R₃ is H, F, Cl, Br, I, CN, NO₂, COR, COOH, CONHR, CF₃, or Sn(R)₃;Q is H, alkyl, halogen, CF₃, CN, C(R)₃, Sn(R)₃, N(R)₂, NHCOCH₃, NHCOCF₃,NHCOR, NHCONHR, NHCOOR, OCONHR, CONHR, NHCSCH₃, NHCSCF₃, NHCSR,NHSO₂CH₃, NHSO₂R, OH, OR, COR, OCOR, OSO₂R, SO₂R, or SR;n is an integer of 1-4; andm is an integer of 1-3;or its isomer, pharmaceutically acceptable salt, crystal, N-oxide,hydrate or any combination thereof to said subject.

In one embodiment, the present invention provides a compound of formulaIII wherein X is O. In another embodiment, the present inventionprovides a compound of formula III wherein T is OH. In anotherembodiment, the present invention provides a compound of formula IIIwherein R₁ is CH₃. In another embodiment, the present invention providesa compound of formula III or IIIA wherein Z is CN. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein Z is F. In another embodiment, the present inventionprovides a compound of formula III or IIIA wherein Z is NO₂. In anotherembodiment, the present invention provides a compound of formula III orIIIA, wherein Y is CH₃. In another embodiment, the present inventionprovides a compound of formula III or IIIA, wherein Y is H. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein Y is CF₃. In another embodiment, the present inventionprovides a compound of formula III or IIIA, wherein Y is Cl. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein R₃ is H and none of Y, Z, Q or R₂ are H. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein R₃ is ON. In another embodiment, the present inventionprovides a compound of formula III or IIIA wherein R₃ is Cl. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein R₃ is F. In another embodiment, the present inventionprovides a compound of formula III or IIIA wherein Q is CN. In anotherembodiment, the present invention provides a compound of formula III orIIIA wherein Q is F. In another embodiment, the present inventionprovides a compound of formula III or IIIA wherein Q is Cl. In anotherembodiment, if R₃ of formula III or IIIA is H, then none of Z or Y or R₂or Q are H.

In one embodiment, the present invention provides a compoundcharacterized by the structure of formula IV:

wherein R₃, m and n are as described for the structure of formula III.

In one embodiment, this invention provides a compound of formulaS-XXIII:

In one embodiment, this invention provides a compound of formula XXIV:

In one embodiment, this invention provides a compound of formula XXV:

In one embodiment, this invention provides an analog of the compound offormulas I-XXV. In another embodiment, this invention provides aderivative of the compound of formulas I-XXV. In another embodiment,this invention provides a prodrug of the compound of formulas I-XXV. Inanother embodiment, this invention provides a metabolite of the compoundof formulas I-XXV. In another embodiment, this invention provides apharmaceutically acceptable salt of the compound of formulas I-XXV. Inanother embodiment, this invention provides a pharmaceutical product ofthe compound of formulas I-XXV. In another embodiment, this inventionprovides a hydrate of the compound of formulas I-XXV. In anotherembodiment, this invention provides an N-oxide of the compound offormulas I-XXV. In another embodiment, this invention provides apolymorph of the compound of formulas I-XXV. In another embodiment, thisinvention provides a crystal of the compound of formulas I-XXV. Inanother embodiment, this invention provides an impurity of the compoundof formulas I-XXV. In another embodiment, this invention provides acombination of any of an analog, derivative, metabolite, isomer,prodrug, pharmaceutically acceptable salt, pharmaceutical product,polymorph, crystal, impurity, hydrate, and N-oxide of the compound offormulas I-XXV.

As contemplated herein, the present invention relates to the use of aSARM compound and/or its analog, derivative, isomer, metabolite,pharmaceutically acceptable salt, pharmaceutical product, hydrate,N-oxide, polymorph, crystal, impurity or combinations thereof. In oneembodiment, the invention relates to the use of an analog of the SARMcompound. In another embodiment, the invention relates to the use of aderivative of the SARM compound. In another embodiment, the inventionrelates to the use of an isomer of the SARM compound. In anotherembodiment, the invention relates to the use of a metabolite of the SARMcompound. In another embodiment, the invention relates to the use of apharmaceutically acceptable salt of the SARM compound. In anotherembodiment, the invention relates to the use of a pharmaceutical productof the SARM compound. In another embodiment, the invention relates tothe use of a hydrate of the SARM compound. In another embodiment, theinvention relates to the use of an N-oxide of the SARM compound. Inanother embodiment, the invention relates to the use of a polymorph ofthe SARM compound. In another embodiment, the invention relates to theuse of a crystal of the SARM compound. In another embodiment, theinvention relates to the use of an impurity of the SARM compound.

As defined herein, the term “isomer” includes, but is not limited to,optical isomers and analogs, structural isomers and analogs,conformational isomers and analogs, and the like. In one embodiment, theterm “isomer” is meant to encompass optical isomers of the SARMcompound. It will be appreciated by those skilled in the art that theSARMs of the present invention contain at least one chiral center.Accordingly, the SARMs used in the methods of the present invention mayexist in, and be isolated in, optically-active or racemic forms. Somecompounds may also exhibit polymorphism. It is to be understood that thepresent invention encompasses any racemic, optically-active,polymorphic, or stereoisomeric form, or mixtures thereof, which formpossesses properties useful in the treatment of androgen-relatedconditions described herein. In one embodiment, the SARMs are the pure(R)-isomers. In another embodiment, the SARMs are the pure (S)-isomers.In another embodiment, the SARMs are a mixture of the (R) and the (S)isomers. In another embodiment, the SARMs are a racemic mixturecomprising an equal amount of the (R) and the (S) isomers. It is wellknown in the art how to prepare optically-active forms (for example, byresolution of the racemic form by recrystallization techniques, bysynthesis from optically-active starting materials, by chiral synthesis,or by chromatographic separation using a chiral stationary phase).

In one embodiment, this invention encompasses the use of various opticalisomers of the SARM compound. It will be appreciated by those skilled inthe art that the SARMs of the present invention contain at least onechiral center. Accordingly, the SARMs used in the methods of the presentinvention may exist in, and be isolated in, optically-active or racemicforms. Some compounds may also exhibit polymorphism. It is to beunderstood that the present invention encompasses any racemic,optically-active, polymorphic, or stereoisomeric form, or mixturesthereof, which form possesses properties useful in the treatment ofandrogen-related conditions described herein. In one embodiment, theSARMs are the pure (R)-isomers. In another embodiment, the SARMs are thepure (S)-isomers. In another embodiment, the SARMs are a mixture of the(R) and the (S) isomers. In another embodiment, the SARMs are a racemicmixture comprising an equal amount of the (R) and the (S) isomers. It iswell known in the art how to prepare optically-active forms (forexample, by resolution of the racemic form by recrystallizationtechniques, by synthesis from optically-active starting materials, bychiral synthesis, or by chromatographic separation using a chiralstationary phase).

The invention includes “pharmaceutically acceptable salts” of thecompounds of this invention, which may be produced, by reaction of acompound of this invention with an acid or base.

Suitable pharmaceutically-acceptable salts of amines of formulas I-XXVmay be prepared from an inorganic acid or from an organic acid. In oneembodiment, examples of inorganic salts of amines are bisulfates,borates, bromides, chlorides, hemisulfates, hydrobromates,hydrochlorates, 2-hydroxyethylsulfonates (hydroxyethanesulfonates),iodates, iodides, isothionates, nitrate, persulfates, phosphate,sulfates, sulfamates, sulfanilates, sulfonic acids (alkylsulfonates,arylsulfonates, halogen substituted alkylsulfonates, halogen substitutedarylsulfonates), sulfonates and thiocyanates.

In one embodiment, examples of organic salts of amines may be selectedfrom aliphatic, cycloaliphatic, aromatic, araliphatic, heterocyclic,carboxylic and sulfonic classes of organic acids, examples of which areacetates, arginines, aspartates, ascorbates, adipates, anthranilates,algenates, alkane carboxylates, substituted alkane carboxylates,alginates, benzenesulfonates, benzoates, bisulfates, butyrates,bicarbonates, bitartrates, carboxylates, citrates, camphorates,camphorsulfonates, cyclohexylsulfamates, cyclopentanepropionates,calcium edetates, camsylates, carbonates, clavulanates, cinnamates,dicarboxylates, digluconates, dodecylsulfonates, dihydrochlorides,decanoates, enanthuates, ethanesulfonates, edetates, edisylates,estolates, esylates, fumarates, formates, fluorides, galacturonates,gluconates, glutamates, glycolates, glucorates, glucoheptanoates,glycerophosphates, gluceptates, glycollylarsanilates, glutarates,glutamates, heptanoates, hexanoates, hydroxymaleates, hydroxycarboxlicacids, hexylresorcinates, hydroxybenzoates, hydroxynaphthoates,hydrofluorate, lactates, lactobionates, laurates, malates, maleates,methylenebis(beta-oxynaphthoate), malonates, mandelates, mesylates,methane sulfonates, methylbromides, methylnitrates, methylsulfonates,monopotassium maleates, mucates, monocarboxylates, nitrates,naphthalenesulfonates, 2-naphthalenesulfonates, nicotinates, napsylates,N-methylglucamines, oxalates, octanoates, oleates, pamoates,phenylacetates, picrates, phenylbenzoates, pivalates, propionates,phthalates, phenylacetate, pectinates, phenylpropionates, palmitates,pantothenates, polygalacturates, pyruvates, quinates, salicylates,succinates, stearates, sulfanilate, subacetates, tartarates,theophyllineacetates, p-toluenesulfonates (tosylates),trifluoroacetates, terephthalates, tannates, teoclates, trihaloacetates,triethiodide, tricarboxylates, undecanoates and valerates.

In one embodiment, examples of inorganic salts of carboxylic acids orphenols may be selected from ammonium, alkali metals to include lithium,sodium, potassium, or cesium; alkaline earth metals to include calcium,magnesium, or aluminium; zinc, barium, cholines, or quaternaryammoniums.

In another embodiment, examples of organic salts of carboxylic acids orphenols may be selected from arginine, organic amines to includealiphatic organic amines, alicyclic organic amines, aromatic organicamines, benzathines, t-butylamines, benethamines(N-benzylphenethylamine), dicyclohexylamines, dimethylamines,diethanolamines, ethanolamines, ethylenediamines, hydrabamines,imidazoles, lysines, methylamines, meglamines, N-methyl-D-glucamines,N,N′-dibenzylethylenediamines, nicotinamides, organic amines,ornithines, pyridines, picolines, piperazines, procain,tris(hydroxymethyl)methylamines, triethylamines, triethanolamines,trimethylamines, tromethamines and ureas.

In one embodiment, the salts may be formed by conventional means, suchas by reacting the free base or free acid form of the product with oneor more equivalents of the appropriate acid or base in a solvent ormedium in which the salt is insoluble or in a solvent such as water,which is removed in vacuo or by freeze drying or by exchanging the ionsof a existing salt for another ion or suitable ion-exchange resin.

In one embodiment, the invention also includes N-oxides of the aminosubstituents of the compounds described herein. Also, esters of thephenolic compounds can be made with aliphatic and aromatic carboxylicacids, for example, acetic acid and benzoic acid esters.

This invention provides derivatives of the SARM compounds. In oneembodiment, “derivatives” includes but is not limited to etherderivatives, acid derivatives, amide derivatives, ester derivatives andthe like. In another embodiment, this invention further includeshydrates of the SARM compounds.

In one embodiment, “hydrate” includes but is not limited to hemihydrate,monohydrate, dihydrate, trihydrate and the like.

This invention provides, in other embodiments, metabolites of the SARMcompounds. In one embodiment, “metabolite” means any substance producedfrom another substance by metabolism or a metabolic process.

This invention provides, in other embodiments, pharmaceutical productsof the SARM compounds. The term “pharmaceutical product” refers, inother embodiments, to a composition suitable for pharmaceutical use(pharmaceutical composition), for example, as described herein.

An “alkyl” group refers, in one embodiment, to a saturated aliphatichydrocarbon, including straight-chain, branched-chain and cyclic alkylgroups. In one embodiment, the alkyl group has 1-12 carbons. In anotherembodiment, the alkyl group has 1-7 carbons. In another embodiment, thealkyl group has 1-6 carbons. In another embodiment, the alkyl group has1-4 carbons. The alkyl group may be unsubstituted or substituted by oneor more groups selected from halogen, hydroxy, alkoxy carbonyl, amido,alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamino,carboxyl, thio and thioalkyl. In one embodiment, the alkyl group is CH₃.

An “alkenyl” group refers, in another embodiment, to an unsaturatedhydrocarbon, including straight chain, branched chain and cyclic groupshaving one or more double bond. The alkenyl group may have one doublebond, two double bonds, three double bonds, etc. Examples of alkenylgroups are ethenyl, propenyl, butenyl, cyclohexenyl, etc. In oneembodiment, the alkylene group has 1-12 carbons. In another embodiment,the alkylene group has 1-7 carbons. In another embodiment, the alkylenegroup has 1-6 carbons. In another embodiment, the alkylene group has 1-4carbons. The alkenyl group may be unsubstituted or substituted by one ormore groups selected from halogen, hydroxy, alkoxy carbonyl, amido,alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamino,carboxyl, thio and thioalkyl.

A “haloalkyl” group refers to an alkyl group as defined above, which issubstituted by one or more halogen atoms, in one embodiment by F, inanother embodiment by Cl, in another embodiment by Br, in anotherembodiment by I.

An “aryl” group refers to an aromatic group having at least onecarbocyclic aromatic group or heterocyclic aromatic group, which may beunsubstituted or substituted by one or more groups selected fromhalogen, haloalkyl, hydroxy, alkoxy carbonyl, amido, alkylamido,dialkylamido, nitro, amino, alkylamino, dialkylamino, carboxy or thio orthioalkyl. Nonlimiting examples of aryl rings are phenyl, naphthyl,pyranyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyrazolyl, pyridinyl,furanyl, thiophenyl, thiazolyl, imidazolyl, isoxazolyl, and the like. Inone embodiment, the aryl group is a 4-8 membered ring. In anotherembodiment, the aryl group is a 4-12 membered ring(s). In anotherembodiment, the aryl group is a 6 membered ring. In another embodiment,the aryl group is a 5 membered ring. In another embodiment, the arylgroup is 2-4 fused ring system.

A “hydroxyl” group refers to an OH group. It is understood by a personskilled in the art that when T is OR, R is not OH.

In one embodiment, the term “halogen” refers to in one embodiment to F,in another embodiment to Cl, in another embodiment to Br, in anotherembodiment to I.

An “arylalkyl” group refers, in another embodiment, to an alkyl bound toan aryl, wherein alkyl and aryl are as defined above. An example of anarylalkyl group is a benzyl group.

In another embodiment, the present invention provides process forpreparing a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula III:

-   -   wherein        -   X is a bond, O, CH₂, NH, Se, PR, NO or NR;        -   G is O or S;        -   T is OH, OR, —NHCOCH₃, or NHCOR;        -   R is alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH₂F,            CHF₂, CF₃, CF₂CF₃, aryl, phenyl, halogen, alkenyl or OH;        -   R₁ is CH₃, CH₂F, CHF₂, CF₃, CH₂CH₃, or CF₂CF₃;        -   R₂ is H, F, Cl, Br, I, CH₃, CF₃, OH, CN, NO₂, NHCOCH₃,            NHCOCF₃, NHCOR, alkyl, arylalkyl, OR, NH₂, NHR, N(R)₂, or            SR;        -   R₃ is H, F, Cl, Br, I, CN, NO₂, COR, COOH, CONHR, CF₃, or            Sn(R)₃; or            -   R₃ together with the benzene ring to which it is                attached forms a fused ring system represented by the                structure:

-   -   -   Z is NO₂, CN, Cl, F, Br, I, H, COR, COOH, or CONHR;        -   Y is CF₃, alkoxy, alkyl, hydroxyalkyl, alkylaldehyde,            formyl, H, F, Br, Cl, I, CN, or Sn(R)₃;        -   Q is H, alkyl, halogen, CF₃, CN, C(R)₃, Sn(R)₃, N(R)₂,            NHCOCH₃, NHCOCF₃, NHCOR, NHCONHR, NHCOOR, OCONHR, CONHR,            NHCSCH₃, NHCSCF₃, NHCSR, NHSO₂CH₃, NHSO₂R, OH, OR, COR,            OCOR, OSO₂R, SO₂R, or SR; or Q together with the benzene            ring to which it is attached is a fused ring system            represented by structure A, B or C:

-   -   -   n is an integer of 1-4; and        -   m is an integer of 1-3;

    -   the process comprising the step of coupling a compound of        formula (10):

wherein Z, Y, G, R₁, T, R₃ and m are as defined above and L is a leavinggroup, with a compound of formula 11:

wherein Q, X, R₂ and n are as defined above.

In one embodiment, the coupling step is carried out in the presence of abase. In another embodiment, the leaving group L is Br.

In another embodiment, the compound of formula 10 is prepared by:

a) preparing a compound of formula 13 by ring opening of a cycliccompound of formula 12:

wherein L, R₁, G and T are as defined above, and T₁ is O or NH; and

b) reacting an amine of formula 14:

wherein Z, Y, R₃ and m are as defined above, with the compound offormula 13, in the presence of a coupling reagent, to produce thecompound of formula 10.

It is understood to a person skilled in the art that when T₁ is O or NH,T in compound 13 is O or NH₂. Thus, when T in compound 13 is OR, thereaction will involve a further step of converting the OH to OR by areaction with, for example, an alkyl halide R-X. When T in compound 13is NHCOR, NHCOCH₃, the reaction will involve a further step ofconverting the NH₂ to NHCOR or NHCOCH₃, by a reaction with, for example,the corresponding acyl chloride ClCOR or ClCOCH₃.

In one embodiment, step (a) is carried out in the presence of HBr.

In one embodiment, whereby compound 13 of step (a) is reacted with acoupling agent prior to step (b).

In one embodiment, the coupling step is carried out in the presence of abase. In another embodiment, the leaving group L is Br.

In another embodiment, the process further comprises the step ofconverting the selective androgen receptor modulator (SARM) compound toits analog, isomer, metabolite, derivative, pharmaceutically acceptablesalt, pharmaceutical product, polymorph, crystal, impurity, N-oxide,hydrate or any combination thereof.

In another embodiment, this invention provides a large scale process forthe preparation of compound of formula III, wherein the processcomprises the same steps as described herein above, wherein compound offormula 12 is prepared according to the following scheme, in thepresence of 4N NaOH:

FIGS. 1K and 1L provide one embodiment of a large scale process for thepreparation of a large scale synthesis of compounds of formulas S-II.

In one embodiment, the present invention provides a process forpreparing a compound of formula III wherein is X is O. In anotherembodiment, the present invention provides a process for preparing acompound of formula III wherein T is OH. In another embodiment, thepresent invention provides a process for preparing a compound of formulaIII wherein is R₁ is CH₃. In another embodiment, the present inventionprovides a process for preparing a compound of formula III wherein Z isCN, Cl or F. In another embodiment, the present invention provides aprocess for preparing a compound of formula III, wherein Z is CN. Inanother embodiment, the present invention provides a process forpreparing a compound of formula III wherein Y is CF₃, CH₃, or H or Cl.In another embodiment, the present invention provides a process forpreparing a compound of formula III wherein R₃ is H, CN, or Cl and/or F.In another embodiment, the present invention provides a process forpreparing a compound of formula III wherein Q is CN. In anotherembodiment, the present invention provides a process for preparing acompound of formula III wherein Q is F. In another embodiment, thepresent invention provides a process for preparing a compound of formulaIII wherein Q is Cl.

In another embodiment, the present invention provides a process forpreparing a selective androgen modulator compound represented by thestructure of formula II, as depicted in FIG. 1 and Example 1:

In another embodiment, the present invention provides a process forpreparing an (S) enantiomer of SARM compound represented by thestructure of formula S-II:

said process comprising the steps of:a) coupling an amine of formula 17:

with the carboxylic acid of formula R-18

in the presence of a coupling reagent, to produce an amide of formulaR-19

b) reacting the amide of formula R-19 with a compound of formula 20:

to produce a compound of formula S-II.

In one embodiment, whereby compound R-18 of step (a) is reacted with acoupling agent prior to addition of compound of formula 17.

FIG. 1A and Example 1 provide one embodiment of a process for thepreparation of a compound of formula S-II.

In another embodiment, the conditions of step (b) of the processoutlined hereinabove may comprise potassium carbonate, sodium carbonate,or cesium carbonate, or another base appropriate for this reaction,using 2-propanol, THF or methylethylketone as a solvent, optionally witha transition catalyst, BTBAC (benzyltributylammonium chloride) or othersuitable agent.

In another embodiment, the present invention provides a process forpreparing an (R) enantiomer of SARM compound represented by thestructure of formula R-II:

said process comprising the steps of:

a) coupling an amine of formula 17:

with the carboxylic acid of formula S-18

in the presence of a coupling reagent, to produce an amide of formulaS-19

b) reacting the amide of formula S-19 with a compound of formula 20

to produce a compound of R-II.

In one embodiment, whereby compound S-18 of step (a) is reacted with acoupling agent prior to addition of compound of formula 17.

FIG. 1B depicts one embodiment of such a process for the preparation ofcompound of formula R-II.

In another embodiment, the conditions of step (b) of the processoutlined hereinabove may comprise potassium carbonate, sodium carbonate,or cesium carbonate, or another base appropriate for this reaction,using 2-propanol, THF or methylethylketone as a solvent, optionally witha transition catalyst, BTBAC (benzyltributylammonium chloride) or othersuitable agent.

In another embodiment, the present invention provides a process forpreparing an (S) enantiomer of a SARM compound represented by thestructure of formula S-II

said process comprising the steps of:

a) coupling an amine of formula 17:

with the carboxylic acid of formula R-18

in the presence of a coupling reagent, to produce an amide of formulaR-19

b) reacting the amide of formula R-19, with a base to form an oxiraneS-21

c) reacting the oxirane of formula S-21 with a compound of formula 20:

to produce a compound of S-II.

In one embodiment, whereby compound R-18 of step (a) is reacted with acoupling agent prior to addition of compound of formula 17.

FIG. 1C depicts an embodiment of such a process for the preparation ofcompound of formula S-II.

In another embodiment, the present invention provides a process forpreparing an (R) enantiomer of SARM compound represented by thestructure of formula R-II:

said process comprising the steps of:

a) coupling an amine of formula 17:

with the carboxylic acid of formula S-18

in the presence of a coupling reagent, to produce an amide of formulaS-19

b) reacting the amide of formula S-19, with a base to form an oxiraneR-21

c) reacting the oxirane of formula R-21 with a compound of formula 20;

to produce a compound of R-II.

In one embodiment, whereby compound S-18 of step (a) is reacted with acoupling agent prior to addition of compound of formula 17.

FIG. 1D depicts preparation of compound of formula R-II.

In another embodiment, the present invention provides a process forpreparing an (S) enantiomer of a SARM compound represented by thestructure of formula S-II.

said process comprising the steps of:

a) reacting a ring of formula S-22

with a compound of 20

to produce a compound of formula R-23;

b) ring opening of compound of formula R-23 to produce a compound offormula S-24

c) coupling the carboxylic acid of compound of formula S-24 with theamine of formula 17

to produce the compound of formula S-II.

FIG. 1E depicts an embodiment of such a process for the preparation ofcompound of formula S-II.

In another embodiment, the present invention provides a process forpreparing an (R) enantiomer of a SARM compound represented by thestructure of formula R-II:

said process comprising the steps of:

a) reacting a ring of formula R-22

with a compound of 20

to produce a compound of formula S-23;

b) ring opening of compound of formula S-23 to produce a compound offormula R-24

c) coupling the carboxylic acid of compound of formula R-24 with theamine of formula 17

to produce the compound of formula R-II.

FIG. 1F depicts an embodiment of such a process for the preparation ofcompound of formula R-II.

In another embodiment, the present invention provides a process forpreparing an (S) enantiomer of a SARM compound represented by thestructure of formula S-II

said process comprising the steps of:

a) reacting the carboxylic acid of formula R-18

with tribromoacetaldehyde to produce a compound of formula R-25:

b) reacting the dioxalane derivative R-25 with a compound of formula 20

to produce a compound of formula R-26;

c) ring opening of compound of formula R-26 to produce a compound offormula S-24

d) coupling the carboxylic acid of compound of formula S-24 with theamine of formula 17:

to produce the compound of formula S-II.

FIG. 1G depicts an embodiment of such a process for the preparation ofcompound of formula S-II.

In another embodiment, the present invention provides a process forpreparing an (R) enantiomer of a SARM compound represented by thestructure of

said process comprising the steps of:

a) reacting the carboxylic acid of formula S-18

with tribromoacetaldehyde to produce a compound of formula S-25:

b) reacting the dioxalane derivative S-25 with a compound of formula 20

to produce a compound of formula S-26;

c) ring opening of compound of formula S-26 to produce a compound offormula R-24

d) coupling the carboxylic acid of compound of formula R-24 with theamine of formula 17:

to produce the compound of formula R-II.

FIG. 1H depicts an embodiment of such a process for the preparation ofcompound of formula R-II.

In another embodiment, the present invention provides a process forpreparing a racemic mixture of a SARM compound represented by thestructure of formula II

said process comprising the steps of:

a) reacting a compound of formula 24

with a compound of formula 27

wherein P is selected from isocyanate (NCO) or isothiocyanate (NCS) toproduce a compound of formula 28a or 28b, respectively

b) ring opening of the oxazolidinedione or 2-thioxooxazolid-4-one ringof formula 28a or 28b in a presence of a base to produce a compound offormula II.

FIG. 1I depicts an embodiment of such a process for the preparation ofracemic compound of formula II.

In another embodiment, the present invention provides a process forpreparing a racemic mixture of a SARM compound represented by thestructure of formula II:

said process comprising the steps of:

a) chlorinating methacrylic acid:

b) coupling an 3-cyano 4-trifluoromethyl aniline of formula 17 withmethacryloyl chloride:

to produce the amide of formula 29,

c) oxidizing an amide of formula 29, to produce the oxirane of formula21:

d) reacting the oxirane of formula 21 with a compound of formula 20:

to produce the compound of formula II.

In another embodiment, the oxidizing an amide of formula 29 of step (c)comprises ozone. In another embodiment, the oxidizing agent is aperoxyacid, for example, peracetic acid, (CH₃COOOH). In anotherembodiment, the oxidizing agent meta-chloroperbenzoic acid (m-CPBA). Inanother embodiment, the oxidizing agent is magnesium monoperoxypthalicacid (MMPP). In another embodiment, the oxidizing agent is hydrogenperoxide together with catalytic amounts (1.0-0.1 mol %) of manganese(2⁺) salts.

FIG. 1J depicts an embodiment of a process for the preparation ofracemic compound of formula II.

In one embodiment, this invention provides a process for preparing pureenantiomers of SARMs compounds of this invention, comprising the stepsof: a) preparing a racemic SARM compound of this invention; and b)separating pure SARM compound of this invention from its racemicmixture.

In one embodiment, separation of the optically-active (R) isomer or (S)enantiomer, from the racemic SARM compounds of this invention comprisescrystallization techniques. In another embodiment, the crystallizationtechniques include differential crystallization of enantiomers. Inanother embodiment, the crystallization techniques include differentialcrystallization of diastereomeric salts (tartaric salts or quininesalts). In another embodiment, the crystallization techniques includedifferential crystallization of chiral auxiliary derivatives (mentholesters, etc). In another embodiment, separation of the optically-active(R) isomer or (S) enantiomer, from the racemic SARM compounds of thisinvention comprises reacting the racemate mixture with another chiralgroup, forming of a diastereomeric mixture followed by separation of thediastereomers and removing the additional chiral group to obtain pureenantiomers. In another embodiment, separation of the optically-active(R) isomer or (S) enantiomer, from the racemic SARM compounds of thisinvention comprises chiral synthesis. In another embodiment, separationof the optically-active (R) isomer or (S) enantiomer, from the racemicSARM compounds of this invention comprises biological resolution. Inanother embodiment, separation of the optically-active (R) isomer or (S)enantiomer, from the racemic SARM compounds of this invention comprisesenzymatic resolution. In another embodiment, separation of theoptically-active (R) isomer or (S) enantiomer, from the racemic SARMcompounds of this invention comprises chromatographic separation using achiral stationary phase. In another embodiment, separation of theoptically-active (R) isomer or (S) enantiomer, from the racemic SARMcompounds of this invention comprises affinity chromatography. Inanother embodiment, separation of the optically-active (R) isomer or (S)enantiomer, from the racemic SARM compounds of this invention comprisescapillary electrophoresis. In another embodiment, separation of theoptically-active (R) isomer or (S) enantiomer, from the racemic SARMcompounds of this invention comprises forming an ester group of thehydroxyl group of the chiral carbon with an optically-active acid, forexample (−)-camphanic acid, separating the diastereomers esters, thusobtained, by fractional crystallization or preferably, byflash-chromatography, and then hydrolyzing each separate ester to thealcohol.

In another embodiment, the purity, and selectivity of an enantiomerobtained by the process of this invention, or by chiral separation of aracemic mixture of this invention can be determined by HPLC analysis.

In another embodiment, the process further comprises the step ofconverting the SARM compound to its analog, isomer, metabolite,derivative, pharmaceutically acceptable salt, pharmaceutical product,N-oxide, hydrate or any combination thereof.

According to this aspect of the invention, and in one embodiment, thereagent used for reacting the amide derivative, for example compound offormula 19 and the phenol derivative such as for example 20 are carriedout in the presence of a base. Any suitable base that will deprotonatethe hydrogen of the —XH moiety (for example, a phenol moiety when X isO) and allow the coupling may be used. Nonlimiting examples of bases arecarbonates such as alkali carbonates, for example sodium carbonate(Na₂CO₃), potassium carbonate (K₂CO₃) and cesium carbonate (Cs₂CO₃);bicarbonates such as alkali metal bicarbonates, for example sodiumbicarbonate (NaHCO₃), potassium bicarbonate (KHCO₃), alkali metalhydrides such as sodium hydride (NaH), potassium hydride (KH) andlithium hydride (LiH), and the like.

The leaving group L, according to this aspect, and in one embodiment,may comprise any removable group customarily considered for chemicalreactions, as will be known to the person skilled in the art. Suitableleaving groups are halogens, for example F, Cl, Br and I; alkylsulfonate esters (—OSO₂R) wherein R is an alkyl group, for examplemethanesulfonate (mesylate), trifluoromethanesulfonate, ethanesulfonate,2,2,2-trifluoroethanesulfonate, perfluoro butanesulfonate; arylsulfonate esters (—OSO₂Ar) wherein Ar is an aryl group, for examplep-toluoylsulfonate (tosylate), benzenesulphonate which may beunsubstituted or substituted by methyl, chlorine, bromine, nitro and thelike; NO₃, NO₂, or sulfate, sulfite, phosphate, phosphite, carboxylate,imino ester, N₂ or carbamate.

According to this aspect of the invention and in one embodiment, thereaction is carried out in a suitable inert solvent or diluent such as,for example, tetrahydrofuran, diethyl ether, acetone, methyl ethylketone, 2-propanol, aromatic amines such as pyridine; aliphatic andaromatic hydrocarbons such as benzene, toluene, and xylene;dimethylsulfoxide (DMSO), dimethylformamide (DMF), and dimethylacetamide(DMAC). In one embodiment, the reaction may be carried out in a suitableinert solvent or diluent as described hereinabove, suitably in thepresence of a base such as triethylamine, and at a temperature in therange, as described above. In one embodiment, the reaction may becarried out at an appropriate temperature, as will be known to oneskilled in the art, for example, in the range, of −20 to 120° C., or forexample at or near ambient temperature.

The coupling reagent defined hereinabove is a reagent capable of turningthe carboxylic acid/thiocarboxylic acid of formula 24 or 18 into areactive derivative thereof, thus enabling coupling with the respectiveamine to form an amide/thioamide bond. A suitable reactive derivative ofa carboxylic acid/thiocarboxylic acid is, for example, an acylhalide/thioacyl halide, for example an acyl/thioacyl chloride formed bythe reaction of the acid/thioacid and an inorganic acid chloride, forexample thionyl chloride; a mixed anhydride, for example an anhydrideformed by the reaction of the acid and a chloroformate such as isobutylchloroformate; an active ester/thioester, for example an ester formed bythe reaction of the acid and a phenol such as pentafluorophenol, anester such as pentafluorophenyl trifluoroacetate or an alcohol such asmethanol, ethanol, isopropanol, butanol or N-hydroxybenzotriazole; anacyl/thioacyl azide, for example an azide formed by the reaction of theacid/thioacid and azide such as diphenylphosphoryl azide; an acylcyanide/thioacyl cyanide, for example a cyanide formed by the reactionof an acid and a cyanide such as diethylphosphoryl cyanide; or theproduct of the reaction of the acid/thioacid and a carbodiimide such asdicyclohexylcarbodiimide.

It is to be understood that the process may comprise any embodimentdescribed herein, as will be appropriate to produce a SARM of acorresponding formula, as will be appreciated by one skilled in the art.

In one embodiment, the process for preparing a SARM of this inventionmay involve ring opening in the presence of less acidic conditions,which in another embodiment, diminish the likelihood of obtaining SARMcompound mixtures, and provide higher yield and purity of a SARM ofinterest. In one embodiment, the ring opening of a process as describedherein, to produce a carboxylic acid of formula 13, is carried out inthe presence of HBr, which, in one embodiment, is at a concentration ofup to 30%, or in another embodiment, of up to 40%, or in anotherembodiment, is of up to 25%, or in another embodiment, of up to 23%, orin another embodiment, of up to between 20-25%. In one embodiment, theSARMs of this invention may be produced via large-scale synthesis,providing highly pure products in high yields.

In one embodiment, the reaction may be carried out in a suitable inertsolvent or diluent as described hereinabove, suitably in the presence ofa base such as triethylamine, and at a temperature in the range, asdescribed above.

In some embodiments the compounds for use in the methods of thisinvention are nonsteroidal ligands for the androgen receptor and maydemonstrate tissue-selective androgenic and/or anabolic activity. Thesenovel agents are useful in affecting the carcass composition, increasingthe lean mass and/or reducing the fat mass of an animal, reducingpercent fat mass, increasing feed efficiency, increasing average dailygain (ADG), decreasing feed to gain ratio (F:G), increasing musclegrowth, modulation of meat quality, and/or enhancing productive life ofanimals, including feedlot animals, beef cattle and finishing livestock.These novel agents are useful in males for the treatment of a variety ofhormone-related conditions such as sexual dysfunction, decreased sexuallibido, erectile dysfunction, hypogonadism, sarcopenia, osteopenia,osteoporosis, alterations in cognition and mood, depression, anemia,hair loss, obesity, benign prostate hyperplasia and/or prostate cancer.Further, the compounds are useful as adjunct to androgen-deprivationtherapy (ADT) for treating prostate cancer. Further, the compounds areuseful for oral testosterone replacement therapy, and treating prostatecancer. In other embodiments, the compounds are useful for the treatmentof a variety of hormone-related conditions in females including, sexualdysfunction, decreased sexual libido, hypogonadism, sarcopenia,osteopenia, osteoporosis, alterations in cognition and mood, depression,anemia, hair loss, obesity, endometriosis, infertility, breast cancer,uterine cancer and ovarian cancer. In other embodiments, the SARMs areuseful for treating, suppressing, inhibiting or reducing the incidenceof diabetes type II, diabetes type I, glucose intolerance,hyperinsulinemia, insulin resistance, dyslipidemia,hypercholesterolemia, high blood pressure, obesity, fatty liverconditions, diabetic nephropathy, diabetic neuropathy, diabeticretinopathy, cardiovascular disease, atherosclerosis, cerebrovascularconditions and stroke.

In some embodiments, the compounds as described herein are useful inpreventing and treating muscle wasting disorders, bone relateddisorders, and diabetes related disorders.

In some embodiments, the compounds as described herein are useful,either alone or as a combination with beta-agonists as feed composition,pharmaceutical compositions or as methods for affecting the carcasscomposition, increasing the lean mass, reducing the fat mass and/orreducing the percent fat mass, increasing feed efficiency, increasingaverage daily gain (ADG), decreasing feed to gain ratio (F:G) of ananimal. In some embodiment, the compounds as described herein areuseful, either alone or as a combination with beta-agonists as feedcomposition or as methods for increasing the muscle growth of an animal,decreasing time to market (or time to slaughter), increasing carcassweight (or slaughter weight) of a feedlot or finishing animal,modulation of meat quality, enhancing productive life of and/orimproving herd health of animals, including feedlot animals, beef cattleand finishing livestock.

In some embodiments, the compounds as described herein are useful,either alone or as a composition, in males and females for the treatmentof a variety of hormone-related conditions, such as hypogonadism,sarcopenia, erectile dysfunction, lack of libido, osteoporosis andfertility. In some embodiments, the compounds as described herein areuseful in stimulating or promoting or restoring function to variousprocesses, which in turn result in the treatment of the conditions asherein described, including, inter alia, promoting erythropoiesis,osteogenesis, muscle growth, glucose uptake, or insulin secretion;and/or preventing lipidogenesis, clotting, insulin resistance,atherosclerosis, osteoclast activity, and others.

In one embodiment, the methods of this invention make use of thedescribed compound contacting or binding a receptor, and therebymediating the described effects. In some embodiments, the receptor is anuclear receptor, which in one embodiment, is an androgen receptor, orin another embodiment, is an estrogen receptor, or in anotherembodiment, is a progesterone receptor, or in another embodiment, is aglucocorticoid receptor. In some embodiments, the multitude of effectsmay occur simultaneously, as a function of binding to multiple receptorsin the subject. In some embodiments, the tissue selective effects of thecompounds as described herein provide for simultaneous action ondifferent target organs.

Compositions and Methods of Use

In some embodiments, this invention provides methods of use whichcomprise administering a composition comprising the described compounds.In one embodiment, a composition is a pharmaceutical composition. In oneembodiment, a composition is a feed composition. In one embodiment, feedcomposition may be a pharmaceutical composition.

As used herein, “pharmaceutical composition” means a “therapeuticallyeffective amount” of the active ingredient, i.e. the compound of formulaIII, together with a pharmaceutically acceptable carrier or diluent. A“feed composition” means an “effective amount”. A “therapeuticallyeffective amount” and/or an “effective amount” as used herein, refers tothat amount which provides a therapeutic effect or effect on the carcassof the animal for a given condition and administration regimen.

In one embodiment, the present invention encompasses incorporating thecompounds into animal feed. In one embodiment, the present inventionencompasses incorporating the compounds into a feed composition. In someembodiments, the compounds/compositions of this invention may beadministered to any animal as herein described, for example to finishinglivestock. Such administration, in some embodiments, is accomplishedvia, inter alia, supplementation in feeds, feed compositions,formulation into feeds, controlled release implants, topical sprays orcreams/ointments, dissolution in drinking water, rumen-stableformulations to include coatings and derivatives, repeated injection,and other means as will be known to the skilled artisan. In oneembodiment, the present invention encompasses incorporating thecompounds into other typical pharmaceutical administration routes andpharmaceutical compositions as described herein.

As used herein, the term “administering” refers to bringing a subject incontact with a compound of the present invention. As used herein,administration can be accomplished in vitro, i.e. in a test tube, or invivo, i.e. in cells or tissues of living organisms, for example humansand/or animals. In one embodiment, the present invention encompassesadministering the compounds of the present invention to a subject.

In one embodiment, the present invention encompasses administering thecompounds of the present invention via implants. In one embodiment,administering the compounds of the present invention is via controlledrelease implants. In another embodiment of the present invention,administering the compounds of the present invention is via topicaladministration. In one embodiment, topical administration is via atopical spray. In one embodiment, topical administration is via a cream.In one embodiment, topical administration is via an ointment. In oneembodiment, compounds and/or compositions of this invention areadministered via an implant to a pig. In one embodiment, compoundsand/or compositions of this invention are administered via topicaladministration to a pig.

In one embodiment, this invention is directed to a feed composition foran animal comprising a compound of this invention. In one embodiment,this invention is directed to a feed composition for an animalcomprising a compound of formula IIIA or its isomer, pharmaceuticallyacceptable salt, crystal, N-oxide, hydrate or any combination thereof.In one embodiment, this invention is directed to a feed composition foran animal comprising a compound of formula I or its isomer,pharmaceutically acceptable salt, crystal, N-oxide, hydrate or anycombination thereof. In one embodiment this invention is directed to afeed composition for an animal comprising a compound of formula II orits isomer, pharmaceutically acceptable salt, crystal, N-oxide, hydrateor any combination thereof. In one embodiment this invention is directedto a feed composition for an animal comprising a compound of formulaXXIII or its isomer, pharmaceutically acceptable salt, crystal, N-oxide,hydrate or any combination thereof. In one embodiment this invention isdirected to a feed composition for an animal comprising a compound offormula XXIV or its isomer, pharmaceutically acceptable salt, crystal,N-oxide, hydrate or any combination thereof. In one embodiment thisinvention is directed to a feed composition for an animal comprising acompound of formula XXV or its isomer, pharmaceutically acceptable salt,crystal, N-oxide, hydrate or any combination thereof.

The feed composition containing the compounds of this invention can beadministered as additives to the animal feed. In one embodiment, theanimal feed including the feed composition of this invention is providedto the animal once a day. In another embodiment twice a day. In anotherembodiment once to five times a day.

In another embodiment, the feed composition comprises between 0.010-50ppm of a compound of this invention. In another embodiment, the feedcomposition comprises 0.01-1 ppm of a compound of this invention. Inanother embodiment, the feed composition comprises 0.10 ppm of acompound of this invention. In another embodiment, the feed compositioncomprises 1 ppm of a compound of this invention. In another embodiment,the feed composition comprises 3 ppm of a compound of this invention. Inanother embodiment, the feed composition comprises 10 ppm of a compoundof this invention. In another embodiment, the feed composition comprises30 ppm of a compound of this invention.

In one embodiment, the animal is fed with the feed composition of thisinvention after it has reached 60 pounds. In one embodiment, the animalis fed with the feed composition of this invention after it has reached50 pounds. In one embodiment, the animal is fed with the feedcomposition of this invention before it has reached 50 pounds.

In one embodiment, the animal is fed with the feed composition of thisinvention for about ten weeks prior to slaughter. In one embodiment, theanimal is fed with the feed composition of this invention for abouttwenty weeks prior to slaughter. In one embodiment, the animal is fedwith the feed composition of this invention for about a year prior toslaughter.

In another embodiment, the feed composition of this invention comprisesa combination of a compound of this invention and a beta-agonist. Inanother embodiment, the feed composition comprises a compound of formulaII and a beta-agonist. In another embodiment, the feed compositioncomprises a compound of formula XXIII and a beta-agonist. In anotherembodiment, the feed composition comprises a compound of formula XXIVand a beta-agonist. In another embodiment, the feed compositioncomprises a compound of formula XXV and a beta-agonist.

In one embodiment, the animal is raised with a beta-agonist enhanceddiet during a first time period of time and later fed in diet withsubstantially no beta-agonist, but including a compound of thisinvention during a second period of time.

In one embodiment a beta-agonist includes ractopamine hydrochloride(sold under the tradenames Optaflexx® or Paylean®, e.g. and availablefrom Elanco of Greenfield, Ind.) and zilpaterol hydrochloride (soldunder the tradename of Zilmax® available from Invervet of Millsboro,Del.). Other active isomers of other drugs with beta-adrenergicagonistic properties, include for example hexoprenaline, isoprenaline,riniterol, isoetharine, metaproterenol, reproterenol, cimaterol,procaterol, carbuterol, tulobuterol, pibuterol, mabuterol, bitolterol,clenbuterol, and bambuterol. Also included may be tautomers ofbeta-agonists that are under development, such as broxaterol, etanterol,imoxiterol, namiterol, picumeterol, RP 58802, RU 42173 and ZK 90055.Those skilled in the art will also realize that there are manypharmaceutically acceptable salt forms of these drugs, such as forexample sulfate, fumarate, hydrobromide, dihydrochloride,methanesulphonate, hydroxynaphthoate, hydrochloride or whereappropriate, one or other of the hydrate forms thereof.

In one embodiment, the feed composition of this invention is prepared asa dry powder or a granulate and added to the animal feed, such as bymixing. Also, other forms of the additive may also be appropriate. Theadditive can be pre-mixed into the feed according to any of the methodsknown to those skilled in the art, or may be mixed or blended into thefeed at the time of feeding.

In one embodiment, this invention is directed to a pharmaceuticalcomposition for an animal comprising a compound of this invention. Inone embodiment, this invention is directed to a pharmaceuticalcomposition for an animal comprising a compound of formula IIIA or itsisomer, pharmaceutically acceptable salt, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a pharmaceutical composition for an animal comprising a compound offormula I or its isomer, pharmaceutically acceptable salt, crystal,N-oxide, hydrate or any combination thereof. In one embodiment thisinvention is directed to a pharmaceutical composition for an animalcomprising a compound of formula II or its isomer, pharmaceuticallyacceptable salt, crystal, N-oxide, hydrate or any combination thereof.In one embodiment this invention is directed to a pharmaceuticalcomposition for an animal comprising a compound of formula XXIII or itsisomer, pharmaceutically acceptable salt, crystal, N-oxide, hydrate orany combination thereof. In one embodiment this invention is directed toa pharmaceutical composition for an animal comprising a compound offormula XXIV or its isomer, pharmaceutically acceptable salt, crystal,N-oxide, hydrate or any combination thereof. In one embodiment thisinvention is directed to a pharmaceutical composition for an animalcomprising a compound of formula XXV or its isomer, pharmaceuticallyacceptable salt, crystal, N-oxide, hydrate or any combination thereof.

The pharmaceutical composition containing the compounds of thisinvention can be administered as additives to the animal feed. In oneembodiment, the animal feed including the pharmaceutical composition ofthis invention is provided to the animal once a day. In anotherembodiment twice a day. In another embodiment once to five times a day.

In another embodiment, the pharmaceutical composition comprises between0.010-50 ppm of a compound of this invention. In another embodiment, thepharmaceutical composition comprises 0.01-1 ppm of a compound of thisinvention. In another embodiment, the pharmaceutical compositioncomprises 0.10 ppm of a compound of this invention. In anotherembodiment, the pharmaceutical composition comprises 1 ppm of a compoundof this invention. In another embodiment, the pharmaceutical compositioncomprises 3 ppm of a compound of this invention. In another embodiment,the pharmaceutical composition comprises 10 ppm of a compound of thisinvention. In another embodiment, the pharmaceutical compositioncomprises 30 ppm of a compound of this invention.

In one embodiment, the pharmaceutical composition of this invention isin an amount from about 0.0005% to about 0.1% of the weight of theanimal. In another embodiment, the pharmaceutical composition of thisinvention is in an amount from about 0.005% to about 0.01% of the weightof the animal. In another embodiment, the pharmaceutical composition ofthis invention is in an amount from about 0.01% to about 0.05%.

In one embodiment, the methods of this invention are used in a subject,which is a human. In another embodiment, the subject is a mammal. Inanother embodiment, the subject is an animal. In another embodiment thesubject is an invertebrate. In another embodiment the subject is avertebrate. In another embodiment, the animal is a feedlot animal. Inanother embodiment, the animal is a beef cattle. In another embodiment,the animal is a finishing livestock.

For administration to mammals, and particularly humans, it is expectedthat the physician will determine the actual dosage and duration oftreatment, which will be most suitable for an individual and can varywith the age, weight and response of the particular individual.

For administration to mammals, in some embodiments, the presentinvention provides compounds, compositions and methods of use thereoffor the enhanced meat productivity in food animals. In some embodiments,this invention provides compounds, compositions and methods of usethereof for the modulation of appetite for feedlot animals. In someembodiments, this invention provides compounds, compositions and methodsof use thereof for improved feed efficiency.

For administration to mammals, in some embodiments, this inventionprovides compounds, compositions and methods of use thereof fordecreased time to market for feedlot animals. In some embodiments, thisinvention provides compounds, compositions and methods of use thereoffor increased terminal weight of feedlot animals. In some embodiments,this invention provides compounds, compositions and methods of usethereof for decreased time to terminal weight of feedlot animals. Insome embodiments, this invention provides compounds, compositions andmethods of use thereof for increased lean weight of feedlot animals. Insome embodiments, this invention provides compounds, compositions andmethods of use thereof for decreased body fat weight of feedlot animals.In some embodiments, this invention provides compounds, compositions andmethods of use thereof for decreased percent body fat weight of feedlotanimals. In some embodiments, this invention provides compounds,compositions and methods of use thereof for the modulation of meatquality in feedlot animals. In some embodiments, this invention providescompounds, compositions and methods of use thereof for increased meatproduction.

In some embodiments, the term “feedlot animals” refers to, inter alia,any animal the meat of which is considered edible in a given culture orcountry. In some embodiments, such term may include without limitationswine (domestic pig, wild boars), bovine (bison, cattle, yaks), cervids(deer, elk, moose), ovine (sheep/lamb), caprine (goats), lagomorphs(rabbit, pika), avian (chicken, turkey, duck, game birds, emu/ostrich),fish (catfish, tilapia, salmon, red drum), shellfish (crustaceans suchas crab, lobster, shrimp; and mollusks such as clams, octopus, squid),roe (caviar), amphibians (frogs, salamanders), reptiles (snakes, turtle,alligator), canids (dog, fox), felines (cat), equines (horse, donkey,zebras), marsupials (kangaroo, opossum), insects (grasshopper, beetles,larvae), primates (gorilla, monkey), rodents (rat, mouse, squirrel,beaver), cetaceans (whale, dolphin), pinnipeds (walrus, seal),miscellaneous (bear, raccoon, elephant) or others as will be appreciatedby one skilled in the art.

In some embodiments, the term “finishing livestock” refers to, interalia, any animal that is normally fattened for the last few monthsbefore processing. In one embodiment, finishing livestock is a beefcattle. In one embodiment, finishing livestock is a pig. In oneembodiment, finishing livestock is a poultry. In one embodiment,finishing livestock is a farmed fish.

In one embodiment, the compounds, compositions or methods of use thereofmay find application in increasing the yield of all retail productsderived from such feedlot animals. For instance, each of the above foodanimals have different types of tissues and preparations thereof such asfor swine: ham, bacon, sausage, pork bellies, pork chop, ribs, brain,chitterling, tripe, tenderloin, etc.

Feedlot practices often include castration in order to better controlthe behavior of feedlot animals and to improve the quality of the meat(more tender, marbled, and colored). This occurs with a loss ofproductivity which could be offset using nonsteroidal androgens,representing one embodiment of a mechanism whereby the compounds andcomposition find application therein.

In some embodiments, enhancing measures of productivity in feedlotanimals may comprise enhancing the number of animals per litter, littersper breeding animal per year, slaughter head count per breeding animalper year, meat product production (in pounds) per breeding animal peryear, average daily growth in pounds, live weight (in pounds), dressingpercent (% of live weight), dressed weight in pounds, retail meat inpounds per head count, retail meat yield (percent of live weight), orany combination thereof.

In one embodiment, the compounds, compositions or methods of use thereofmay find application in stud farm productivity. Androgens (steroidal andnonsteroidal) are known to enhance sex drive in males and females suchthat, in some embodiments, the stud animals are productive in terms of“open” mating time or births per mating event. In some embodiments, thesupport of sex organs and accessory tissues (and health benefits) of thecompounds/compositions of this invention may increase productive life ofa stud animal, allowing him to “stand at stud” (i.e. meaning availablefor reproduction) for a longer period of time. Female receptivity isenhanced, in some embodiments, in terms of frequency, in response tocontact with/administration of a compound/composition of this invention.

In some embodiments, this invention comprises application of any methodas herein described for veterinary use, in any animal as describedherein. In some embodiments, treatment of such conditions or diseases inanimals may find application for pleasure and/or profit animals, mayincrease the size of game animals by supplementation, etc. as will beappreciated by one skilled in the art.

In some embodiments, the compounds/compositions may be administered toany animal as herein described, for example to livestock. Suchadministration, in some embodiments, is accomplished via, inter alia,supplementation in feeds, formulation into feeds, controlled releaseimplants, dissolution in drinking water, rumen-stable formulations toinclude coatings and derivatives, repeated injection, and other means aswill be known to the skilled artisan.

In some embodiments, dosages as described herein for humans will beadjusted to accommodate the varying size of animals. Such modificationof dosage is well known in the field of veterinary art, and is availablein common veterinary manuals, and may vary on a scale ranging frommilligrams to grams as a function of such varying size.

In some embodiments, the compounds/compositions may be administered toany animal as herein described, in combination with any other agent asdescribed herein, befitting the particular animal and condition in theanimal, which is being treated. In some embodiments, such combinationtherapy may comprise administration of the compounds/compositions withhigh fat diets such as supplemented with fatty acids or oils to improvethe meat quality; various combinations with androgens, progestins,anti-glucocorticoids, estrogens, growth hormone, etc. can be tailored toproduce maximum weight gain performance in different types of animals(cows vs. pigs; intact vs. castrated) the specifics of which are knownby those skilled in the art (see for example, Environ Qual Saf Suppl.1976; (5):89-98).

In some embodiments, the compounds/compositions may be administered toany animal as herein described, which is a food source for humans, andin some embodiments, the tissue-selectivity and shorter half-lives ofthe compounds as herein described significantly lowers anticipatedenvironmental effects. In some embodiments, the risk to humanconsumption thereby, as compared to agricultural use of steroidalandrogens such as trenbolone acetate whose half-life is 3 days, is muchreduced, and comprises therefore an embodiment of an advantage of thecompounds of this invention.

In some embodiments, an advantage of the compounds/compositions of thisinvention may comprise the anabolic activity of the compound therebyproducing larger animals and affecting carcass composition in less time.Factors contributing to the increasing productivity may include, in someembodiments, enhanced mineral (and other nutrient) absorption in thegut; enhanced body protein accretion and metabolism of fat storesresulting in increased lean growth rates; increasing nitrogen uptake bymuscles, leading to an increase in the rate of protein synthesis andmuscle/bone growth.

In some embodiments, the present invention provides a method forenhanced production such as milk, sperm, or egg. In some embodiments,the present invention provides a method for enhanced production of leanmeats or eggs. In some embodiments, the present invention provides amethod for increased productivity of feeds or stud livestock, forexample, increased sperm count, improved morphology of sperm, etc. Insome embodiments, the present invention provides a method for expandingthe productive life of farm animals, for example, egg-laying hens,milk-producing cows, etc, and/or enhanced herd health, for example,improved immune clearance, stronger animals.

In one embodiment, this invention is directed to a method of affectingthe carcass composition of an animal comprising administering a compoundof this invention. In one embodiment, this invention is directed to amethod of affecting the carcass composition of an animal comprisingadministering a compound of formula IIIA or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of affecting the carcass composition of an animal comprisingadministering a compound of formula I or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of affecting the carcass composition of an animal comprisingadministering a compound of formula II or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of affecting the carcass composition of an animal comprisingadministering a compound of formula XXIII its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of affecting the carcass composition of an animal comprisingadministering a compound of formula XXIV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of affecting the carcass composition of an animal comprisingadministering a compound of formula XXV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In another embodiment, the carcass compositionis affected by increasing the lean mass, reducing the fat mass, orreducing percent fat mass. In another embodiment, the carcasscomposition comprises increasing the growth performance in said animal.

In one embodiment, this invention is directed to a method of increasinglean mass of an animal comprising administering a compound of thisinvention. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula IIIA or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula I or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula II or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula XXIII its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula XXIV or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofincreasing lean mass of an animal comprising administering a compound offormula XXV or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof.

In one embodiment, this invention is directed to a method of reducingfat mass of an animal comprising administering a compound of thisinvention. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula IIIA or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula I or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula II or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula XXIII its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula XXIV or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof. In one embodiment, this invention is directed to a method ofreducing fat mass of an animal comprising administering a compound offormula XXV or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof.

In one embodiment, this invention is directed to a method of reducingpercent fat mass of an animal comprising administering a compound ofthis invention. In one embodiment, this invention is directed to amethod of reducing percent fat mass of an animal comprisingadministering a compound of formula IIIA or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of reducing percent fat mass of an animal comprisingadministering a compound of formula I or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of reducing percent fat mass of an animal comprisingadministering a compound of formula II or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of reducing percent fat mass of an animal comprisingadministering a compound of formula XXIII its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of reducing percent fat mass of an animal comprisingadministering a compound of formula XXIV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of reducing percent fat mass of an animal comprisingadministering a compound of formula XXV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof.

In one embodiment, this invention is directed to a method of increasingfeed efficiency of an animal comprising administering a compound of thisinvention. In one embodiment, this invention is directed to a method ofincreasing feed efficiency of an animal comprising administering acompound of formula IIIA or its isomer, pharmaceutically acceptablesalt, pharmaceutical product, crystal, N-oxide, hydrate or anycombination thereof. In one embodiment, this invention is directed to amethod of increasing feed efficiency of an animal comprisingadministering a compound of formula I or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of increasing feed efficiency of an animal comprisingadministering a compound of formula II or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of increasing feed efficiency of an animal comprisingadministering a compound of formula XXIII its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of increasing feed efficiency of an animal comprisingadministering a compound of formula XXIV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof. In one embodiment, this invention is directedto a method of increasing feed efficiency of an animal comprisingadministering a compound of formula XXV or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof.

In one embodiment, this invention is directed to a method of increasingaverage daily gain (ADG) of an animal comprising administering acompound of this invention. In one embodiment, this invention isdirected to a method of increasing average daily gain (ADG) of an animalcomprising administering a compound of formula IIIA or its isomer,pharmaceutically acceptable salt, pharmaceutical product, crystal,N-oxide, hydrate or any combination thereof. In one embodiment, thisinvention is directed to a method of increasing average daily gain (ADG)of an animal comprising administering a compound of formula I or itsisomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of increasing average daily gain(ADG) of an animal comprising administering a compound of formula II orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of increasing average daily gain(ADG) of an animal comprising administering a compound of formula XXIIIits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of increasing average daily gain(ADG) of an animal comprising administering a compound of formula XXIVor its isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of increasing average daily gain(ADG) of an animal comprising administering a compound of formula XXV orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof.

In one embodiment, this invention is directed to a method of decreasingfeed to gain ratio (F:G) of an animal comprising administering acompound of this invention. In one embodiment, this invention isdirected to a method of decreasing feed to gain ratio (F:G) of an animalcomprising administering a compound of formula IIIA or its isomer,pharmaceutically acceptable salt, pharmaceutical product, crystal,N-oxide, hydrate or any combination thereof. In one embodiment, thisinvention is directed to a method of decreasing feed to gain ratio (F:G)of an animal comprising administering a compound of formula I or itsisomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of decreasing feed to gain ratio(F:G) of an animal comprising administering a compound of formula II orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of decreasing feed to gain ratio(F:G) of an animal comprising administering a compound of formula XXIIIits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of decreasing feed to gain ratio(F:G) of an animal comprising administering a compound of formula XXIVor its isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof. In one embodiment,this invention is directed to a method of decreasing feed to gain ratio(F:G) of an animal comprising administering a compound of formula XXV orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof.

In one embodiment the compounds, compositions and methods of thisinvention decrease the fat mass of an animal by 2-15%. In anotherembodiment, decrease the fat mass of an animal by 2-10%. In anotherembodiment, decrease the fat mass of an animal by 5-10%. In anotherembodiment, decrease the fat mass of an animal by 5-15%. In anotherembodiment, the animal is a pig. In another embodiment the animal is abeef cattle. In another embodiment, the animal is a finishing livestock.In another embodiment the animal is a feedlot animal.

In another embodiment the methods and/or compositions of this inventionmake use of the compounds of this invention for decreasing the fat massof an animal by 5-15% after 7-28 days. In another embodiment the methodsand/or compositions of this invention make use of the compounds of thisinvention for decreasing the fat mass of an animal by 5-15% after 7-14days. In another embodiment the methods and/or compositions of thisinvention make use of the compounds of this invention for decreasing thefat mass of an animal by 5-15% after 14-21 days. In another embodimentthe methods and/or compositions of this invention make use of thecompounds of this invention for decreasing the fat mass of an animal by5-15% after 21-28 days. In another embodiment the methods and/orcompositions of this invention make use of the compounds of thisinvention for decreasing the fat mass of an animal by 5-15% after 28-60days.

In one embodiment the compounds, compositions and methods of thisinvention increase lean mass of an animal by 5-15%. In anotherembodiment, increase lean mass of an animal by 5-10%. In anotherembodiment, increase lean mass of an animal by 8-10%. In anotherembodiment, increase lean mass of an animal by 15-30%. In anotherembodiment, the animal is a pig. In another embodiment the animal isbeef cattle. In another embodiment, the animal is a finishing livestock.In another embodiment the animal is a feedlot animal.

In another embodiment the methods and/or compositions of this inventionmake use of the compounds of this invention for increasing lean mass ofan animal by 5-15% after 7-28 days. In another embodiment the methodsand/or compositions of this invention make use of the compounds of thisinvention for increasing lean mass of an animal by 5-15% after 7-14days. In another embodiment the methods and/or compositions of thisinvention make use of the compounds of this invention for increasinglean mass of an animal by 5-15% after 14-21 days. In another embodimentthe methods and/or compositions of this invention make use of thecompounds of this invention for increasing lean mass of an animal by5-15% after 21-28 days. In another embodiment the methods and/orcompositions of this invention make use of the compounds of thisinvention for increasing lean mass of an animal by 5-15% after 28-60days. In another embodiment the methods and/or compositions of thisinvention make use of the compounds of this invention for increasinglean mass of an animal by 15-30% after 7-28 days. In another embodimentthe methods and/or compositions of this invention make use of thecompounds of this invention for increasing lean mass of an animal by15-30% after 7-14 days. In another embodiment the methods and/orcompositions of this invention make use of the compounds of thisinvention for increasing lean mass of an animal by 15-30% after 14-21days. In another embodiment the methods and/or compositions of thisinvention make use of the compounds of this invention for increasinglean mass of an animal by 15-30% after 21-28 days. In another embodimentthe methods and/or compositions of this invention make use of thecompounds of this invention for increasing lean mass of an animal by15-30% after 28-60 days.

In one embodiment, the methods of this invention include administering acompound and/or feeding composition to an animal. In another embodiment,the compound and/or feed composition is provided in the daily feed tothe animal. In another embodiment, the feed composition comprises acompound of this invention. In another embodiment, the feed compositioncomprises a combination of a compound of this invention and abeta-agonist. In another embodiment, the beta-agonist is ractopaminehydrochloride (Paylean®).

The pharmaceutical compositions and feed composition containing thecompounds of this invention can be administered to a subject by anymethod known to a person skilled in the art, such as orally,parenterally, intravascularly, paracancerally, transmucosally,transdermally, intramuscularly, intranasally, intravenously,intradermally, subcutaneously, sublingually, intraperitoneally,intraventricularly, intracranially, intravaginally, by inhalation,rectally, intratumorally, or by any means in which the recombinantvirus/composition can be delivered to tissue (e.g., needle or catheter).Alternatively, topical administration may be desired for application tomucosal cells, for skin or ocular application. Another method ofadministration is via aspiration or aerosol formulation.

In one embodiment, the pharmaceutical compositions are administeredorally, and are thus formulated in a form suitable for oraladministration, i.e. as a solid or a liquid preparation. Suitable solidoral formulations include tablets, capsules, pills, granules, pellets,powders, and the like. Suitable liquid oral formulations includesolutions, suspensions, dispersions, emulsions, oils and the like. Inone embodiment of the present invention, the SARM compounds areformulated in a capsule. In accordance with this embodiment, thecompositions of the present invention comprise in addition to a compoundof this invention and the inert carrier or diluent, a hard gelatincapsule.

In one embodiment, the micronized capsules comprise particles containinga compound of this invention, wherein the term “micronized” used hereinrefers to particles having a particle size is of less than 100 microns,or in another embodiment, less than 60 microns, or in anotherembodiment, less than 36 microns, or in another embodiment, less than 16microns, or in another embodiment, less than 10 microns, or in anotherembodiment, less than 6 microns.

Further, in another embodiment, the pharmaceutical compositions areadministered by intravenous, intraarterial, or intramuscular injectionof a liquid preparation. Suitable liquid formulations include solutions,suspensions, dispersions, emulsions, oils and the like. In oneembodiment, the pharmaceutical compositions are administeredintravenously, and are thus formulated in a form suitable forintravenous administration. In another embodiment, the pharmaceuticalcompositions are administered intraarterially, and are thus formulatedin a form suitable for intraarterial administration. In anotherembodiment, the pharmaceutical compositions are administeredintramuscularly, and are thus formulated in a form suitable forintramuscular administration.

Further, in another embodiment, the pharmaceutical compositions areadministered topically to body surfaces, and are thus formulated in aform suitable for topical administration. Suitable topical formulationsinclude gels, ointments, creams, lotions, drops and the like. Fortopical administration, the compounds of this invention or theirphysiologically tolerated derivatives such as salts, esters, N-oxides,and the like are prepared and applied as solutions, suspensions, oremulsions in a physiologically acceptable diluent with or without apharmaceutical carrier.

Further, in another embodiment, the pharmaceutical compositions areadministered as a suppository, for example a rectal suppository or aurethral suppository. Further, in another embodiment, the pharmaceuticalcompositions are administered by subcutaneous implantation of a pellet.In a further embodiment, the pellet provides for controlled release of acompound as herein described over a period of time. In a furtherembodiment, the pharmaceutical compositions are administeredintravaginally.

In another embodiment, the active compound can be delivered in avesicle, in particular a liposome (see Langer, Science 249:1627-1633(1990); Treat et al., in Liposomes in the Therapy of Infectious Diseaseand Cancer, Lopez-Berestein and Fidler (eds.), Liss, New York, pp.363-366 (1989); Lopez-Berestein, ibid., pp. 317-327; see generallyibid).

As used herein “pharmaceutically acceptable carriers or diluents” arewell known to those skilled in the art. The carrier or diluent may be asolid carrier or diluent for solid formuations, a liquid carrier ordiluent for liquid formulations, or mixtures thereof.

Solid carriers/diluents include, but are not limited to, a gum, a starch(e.g. corn starch, pregeletanized starch), a sugar (e.g., lactose,mannitol, sucrose, dextrose), a cellulosic material (e.g.microcrystalline cellulose), an acrylate (e.g. polymethylacrylate),calcium carbonate, magnesium oxide, talc, or mixtures thereof.

In one embodiment, the compositions of this invention may include, acompound of this invention or any combination thereof, together with oneor more pharmaceutically acceptable excipients.

It is to be understood that this invention encompasses any embodiment ofa compound as described herein, which in some embodiments is referred toas “a compound of this invention”. Such reference will include anycompound, which is characterized by a structure of the formulas I-XXV,or any embodiment thereof, as described herein.

Suitable excipients and carriers may be, according to embodiments of theinvention, solid or liquid and the type is generally chosen based on thetype of administration being used. Liposomes may also be used to deliverthe composition. Examples of suitable solid carriers include lactose,sucrose, gelatin and agar. Oral dosage forms may contain suitablebinders, lubricants, diluents, disintegrating agents, coloring agents,flavoring agents, flow-inducing agents, and melting agents. Liquiddosage forms may contain, for example, suitable solvents, preservatives,emulsifying agents, suspending agents, diluents, sweeteners, thickeners,and melting agents. Parenteral and intravenous forms should also includeminerals and other materials to make them compatible with the type ofinjection or delivery system chosen. Of course, other excipients mayalso be used.

For liquid formulations, pharmaceutically acceptable carriers may beaqueous or non-aqueous solutions, suspensions, emulsions or oils.Examples of non-aqueous solvents are propylene glycol, polyethyleneglycol, and injectable organic esters such as ethyl oleate. Aqueouscarriers include water, alcoholic/aqueous solutions, cyclodextrins,emulsions or suspensions, including saline and buffered media. Examplesof oils are those of petroleum, animal, vegetable, or synthetic origin,for example, peanut oil, soybean oil, mineral oil, olive oil, sunfloweroil, and fish-liver oil.

Parenteral vehicles (for subcutaneous, intravenous, intraarterial, orintramuscular injection) include sodium chloride solution, Ringer's®dextrose, dextrose and sodium chloride, lactated Ringer's® and fixedoils. Intravenous vehicles include fluid and nutrient replenishers,electrolyte replenishers such as those based on Ringer's® dextrose, andthe like. Examples are sterile liquids such as water and oils, with orwithout the addition of a surfactant and other pharmaceuticallyacceptable adjuvants. In general, water, saline, aqueous dextrose andrelated sugar solutions, and glycols such as propylene glycols orpolyethylene glycol are preferred liquid carriers, particularly forinjectable solutions. Examples of oils are those of petroleum, animal,vegetable, or synthetic origin, for example, peanut oil, soybean oil,mineral oil, olive oil, sunflower oil, and fish-liver oil.

In addition, the compositions may further comprise binders (e.g. acacia,cornstarch, gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropylcellulose, hydroxypropyl methyl cellulose, povidone), disintegratingagents (e.g. cornstarch, potato starch, alginic acid, silicon dioxide,croscarmelose sodium, crospovidone, guar gum, sodium starch glycolate),buffers (e.g., Tris-HCl., acetate, phosphate) of various pH and ionicstrength, additives such as albumin or gelatin to prevent absorption tosurfaces, detergents (e.g., Tween® 20, Tween® 80, Pluronic F68®, bileacid salts), protease inhibitors, surfactants (e.g. sodium laurylsulfate), permeation enhancers, solubilizing agents (e.g., Cremophor®,glycerol, polyethylene glycerol, benzlkonium chloride, benzyl benzoate,cyclodextrins, sobitan esters, stearic acids), anti-oxidants (e.g.,ascorbic acid, sodium metabisulfite, butylated hydroxyanisole),stabilizers (e.g. hydroxypropyl cellulose, hyroxypropylmethylcellulose), viscosity increasing agents (e.g. carbomer, colloidalsilicon dioxide, ethyl cellulose, guar gum), sweetners (e.g. aspartame,citric acid), preservatives (e.g., Thimerosal®, benzyl alcohol,parabens), coloring agents, lubricants (e.g. stearic acid, magnesiumstearate, polyethylene glycol, sodium lauryl sulfate), flow-aids (e.g.colloidal silicon dioxide), plasticizers (e.g. diethyl phthalate,triethyl citrate), emulsifiers (e.g. carbomer, hydroxypropyl cellulose,sodium lauryl sulfate), polymer coatings (e.g., poloxamers orpoloxamines), coating and film forming agents (e.g. ethyl cellulose,acrylates, polymethacrylates), and/or adjuvants.

In one embodiment, the pharmaceutical compositions provided herein arecontrolled release compositions, i.e. compositions in which the compoundof this invention is released over a period of time afteradministration. Controlled or sustained release compositions includeformulation in lipophilic depots (e.g. fatty acids, waxes, oils). Inanother embodiment, the composition is an immediate release composition,i.e. a composition in which all of the compound is released immediatelyafter administration.

In yet another embodiment, the pharmaceutical composition can bedelivered in a controlled release system. For example, the agent may beadministered using intravenous infusion, an implantable osmotic pump, atransdermal patch, liposomes, or other modes of administration. In oneembodiment, a pump may be used (see Langer, supra; Sefton, CRC Crit.Ref. Biomed. Eng. 14:201 (1987); Buchwald et al., Surgery 88:607 (1980);Saudek et al., N. Engl. J. Med. 321:674 (1989). In another embodiment,polymeric materials can be used. In yet another embodiment, a controlledrelease system can be placed in proximity to the therapeutic target,i.e., the brain, thus requiring only a fraction of the systemic dose(see, e.g., Goodson, in Medical Applications of Controlled Release,supra, vol. 2, pp. 116-138 (1984). Other controlled release systems arediscussed in the review by Langer (Science 249:1627-1633 (1990).

The compositions may also include incorporation of the active materialinto or onto particulate preparations of polymeric compounds such aspolylactic acid, polglycolic acid, hydrogels, etc, or onto liposomes,microemulsions, micelles, unilamellar or multilamellar vesicles,erythrocyte ghosts, or spheroplasts. Such compositions will influencethe physical state, solubility, stability, rate of in vivo release, andrate of in vivo clearance.

Also comprehended by the invention are particulate compositions coatedwith polymers (e.g. poloxamers or poloxamines) and the compound coupledto antibodies directed against tissue-specific receptors, ligands orantigens or coupled to ligands of tissue-specific receptors.

Also comprehended by the invention are compounds modified by thecovalent attachment of water-soluble polymers such as polyethyleneglycol, copolymers of polyethylene glycol and polypropylene glycol,carboxymethyl cellulose, dextran, polyvinyl alcohol,polyvinylpyrrolidone or polyproline. The modified compounds are known toexhibit substantially longer half-lives in blood following intravenousinjection than do the corresponding unmodified compounds (Abuchowski etal., 1981; Newmark et aL, 1982; and Katre et al., 1987). Suchmodifications may also increase the compound's solubility in aqueoussolution, eliminate aggregation, enhance the physical and chemicalstability of the compound, and greatly reduce the immunogenicity andreactivity of the compound. As a result, the desired in vivo biologicalactivity may be achieved by the administration of such polymer-compoundabducts less frequently or in lower doses than with the unmodifiedcompound.

The preparation of pharmaceutical compositions which contain an activecomponent is well understood in the art, for example by mixing,granulating, or tablet-forming processes. The active therapeuticingredient is often mixed with excipients which are pharmaceuticallyacceptable and compatible with the active ingredient. For oraladministration, the compounds of this invention or their physiologicallytolerated derivatives such as salts, esters, N-oxides, and the like aremixed with additives customary for this purpose, such as vehicles,stabilizers, or inert diluents, and converted by customary methods intosuitable forms for administration, such as tablets, coated tablets, hardor soft gelatin capsules, aqueous, alcoholic or oily solutions. Forparenteral administration, the compounds of this invention or theirphysiologically tolerated derivatives such as salts, esters, N-oxides,and the like are converted into a solution, suspension, or emulsion, ifdesired with the substances customary and suitable for this purpose, forexample, solubilizers or other.

An active component can be formulated into the composition asneutralized pharmaceutically acceptable salt forms. Pharmaceuticallyacceptable salts include the acid addition salts (formed with the freeamino groups of the polypeptide or antibody molecule), which are formedwith inorganic acids such as, for example, hydrochloric or phosphoricacids, or such organic acids as acetic, oxalic, tartaric, mandelic, andthe like. Salts formed from the free carboxyl groups can also be derivedfrom inorganic bases such as, for example, sodium, potassium, ammonium,calcium, or ferric hydroxides, and such organic bases as isopropylamine,trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like.

For use in medicine, the salts of the compound will be pharmaceuticallyacceptable salts. Other salts may, however, be useful in the preparationof the compounds according to the invention or of their pharmaceuticallyacceptable salts. Suitable pharmaceutically acceptable salts of thecompounds of this invention include acid addition salts which may, forexample, be formed by mixing a solution of the compound according to theinvention with a solution of a pharmaceutically acceptable acid such ashydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid,maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid,citric acid, tartaric acid, carbonic acid or phosphoric acid.

In one embodiment, this invention provides pharmaceutical compositionscomprising a compound of this invention. In one embodiment, suchcompositions are useful for oral testosterone replacement therapy.

In one embodiment, this invention also provides a composition comprisingtwo or more compounds of this invention, or polymorphs, isomers,hydrates, salts, N-oxides, etc., thereof. The present invention alsorelates to compositions and pharmaceutical compositions which comprise acompound of this invention alone or in combination with a progestin orestrogen, or in another embodiment, chemotherapeutic compound,osteogenic or myogenic compound, or other agents suitable for theapplications as herein described. In one embodiment, the compositions ofthis invention will comprise a suitable carrier, diluent or salt.

In one embodiment, the methods of this invention may compriseadministration of a compound of this invention at various dosages. Inanother embodiment, the methods of this invention may compriseadministration of a compound of formula II of this invention at variousdosages. In one embodiment, the compound of this invention isadministered at a dosage of 0.1-200 mg per day. In one embodiment, thecompound of this invention is administered at a dose of 0.1-10 mg, or inanother embodiment, 0.1-26 mg, or in another embodiment, 0.1-60 mg, orin another embodiment, 0.3-16 mg, or in another embodiment, 0.3-30 mg,or in another embodiment, 0.6-26 mg, or in another embodiment, 0.6-60mg, or in another embodiment, 0.76-16 mg, or in another embodiment,0.76-60 mg, or in another embodiment, 1-6 mg, or in another embodiment,1-20 mg, or in another embodiment, 3-16 mg, or in another embodiment,30-60 mg, or in another embodiment, 30-76 mg, or in another embodiment,100-2000 mg, or in another embodiment, 1000-20,000 mg.

In one embodiment, the methods of this invention may compriseadministration of a compound of this invention at various dosages. Inanother embodiment, the methods of this invention may compriseadministration of a compound of formula II of this invention at variousdosages. In one embodiment, the compound of this invention isadministered at a dosage of 1 mg. In another embodiment the compound ofthis invention is administered at a dosage of 6 mg, 10 mg, 16 mg, 20 mg,26 mg, 30 mg, 36 mg, 40 mg, 46 mg, 50 mg, 56 mg, 60 mg, 66 mg, 70 mg, 76mg, 80 mg, 86 mg, 90 mg, 96 mg, 100 mg, 200 mg, 500 mg, 1000 mg, 2000mg, 10,000 mg, or 20,000 mg.

In one embodiment, the present invention provides methods of usecomprising the administration of a composition comprising: a) anyembodiment of a compound as described herein; and b) additives, apharmaceutically acceptable carrier or diluent; which is to beunderstood to include an analog, isomer, metabolite, derivative,pharmaceutically acceptable salt, N-oxide, hydrate or any combinationthereof of a compound as herein described, and may comprise compounds offormulas I-XXV.

In some embodiments, the present invention provides methods of use of acomposition comprising: a) any embodiment of the compounds as describedherein, including an analog, isomer, metabolite, derivative,pharmaceutically acceptable salt, pharmaceutical product, N-oxide,hydrate thereof or any combination thereof; b) a pharmaceuticallyacceptable carrier or diluent; c) a flow-aid; and d) a lubricant.

In another embodiment, the present invention provides methods of use ofa composition comprising: a) any embodiment of the compounds asdescribed herein, including an analog, isomer, metabolite, derivative,pharmaceutically acceptable salt, pharmaceutical product, N-oxide,hydrate thereof or any combination thereof; b) lactose monohydrate; c)microcrystalline cellulose; d) magnesium stearate; e) additives; and f)colloidal silicon dioxide.

In some embodiments, the methods of this invention make use ofcompositions comprising compounds of this invention, which offer theadvantage that the compounds are nonsteroidal ligands for the androgenreceptor, and exhibit anabolic activity in vivo. According to thisaspect, such compounds are unaccompanied by serious side effects,provide convenient modes of administration, and lower production costsand are orally bioavailable, lack of significant cross-reactivity withother undesired steroid receptors, and may possess long biologicalhalf-lives.

In one embodiment, the compositions for administration may be sterilesolutions, or in other embodiments, aqueous or non-aqueous, suspensionsor emulsions. In one embodiment, the compositions may comprise propyleneglycol, polyethylene glycol, injectable organic esters, for exampleethyl oleate, or cyclodextrins. In another embodiment, compositions mayalso comprise wetting, emulsifying and/or dispersing agents. In anotherembodiment, the compositions may also comprise sterile water or anyother sterile injectable medium.

In one embodiment, the invention provides compounds and compositions,including any embodiment described herein, for use in any of the methodsof this invention, as described herein. In one embodiment, use of acompound of this invention or a composition comprising the same, willhave utility in inhibiting, suppressing, enhancing or stimulating adesired response in a subject, as will be understood by one skilled inthe art. In another embodiment, the compositions may further compriseadditional active ingredients, whose activity is useful for theparticular application for which the compound of this invention is beingadministered.

In some embodiments, the methods of this invention make use ofcompositions comprising compounds of this invention, which offer theadvantage that the compounds are nonsteroidal ligands for the androgenreceptor, and exhibit anabolic activity in vivo. According to thisaspect, such compounds are unaccompanied by serious side effects,provide convenient modes of administration, and lower production costsand are orally bioavailable, lack significant cross-reactivity withother undesired steroid receptors, and may possess long biologicalhalf-lives.

In some embodiments, the compositions will further comprise a5α-reductase inhibitors (5ARI), a beta-agonist, a SARM or SARMs, aselective estrogen receptor modulator (SERM), an aromatase inhibitor,such as but not limited to anastrazole, exemestane, or letrozole, a GnRHagonist or antagonist, a steroidal or nonsteroidal GR ligand, asteroidal or nonsterodial PR ligand, a steroidal or nonsteroidal ARantagonist, a 17-aldoketoreductase inhibitor or 17β-hydroxysteroiddehydrogenase inhibitor. Such compositions may be used, in someembodiments, for treating a hormone dependent condition, such as, forexample, infertility, neoplasia of a hormone-responsive cancer, forexample, a gonadal cancer, or a urogenital cancer.

In some embodiments, the composition will comprise the compounds asdescribed herein, as well as another therapeutic compound, includinginter alia, a 5ARI such as finasteride, dutasteride, izonsteride; otherSARMs, such as, RU-58642, RU-56279, WS9761 A and B, RU-59063, RU-58841,bexlosteride, LG-2293, L-245976, LG-121071, LG-121091, LG-121104,LGD-2226, LGD-2941, YM-92088, YM-175735, LGD-1331, BMS-357597,BMS-391197, S-40503, BMS-482404, EM-4283, EM-4977, BMS-564929,BMS-391197, BMS-434588, BMS-487745, BMS-501949, SA-766, YM-92088,YM-580, LG-123303, LG-123129, PMCol, YM-175735, BMS-591305, BMS-591309,BMS-665139, BMS-665539, CE-590, 116BG33, 154BG31, arcarine, ACP-105;SERMs, such as tamoxifen, 4-hydroxytamoxifen, idoxifene, toremifene,ospemifene, droloxifene, raloxifene, arzoxifene, bazedoxifene, PPT(1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole), DPN, lasofoxifene,pipendoxifene, EM-800, EM-652, nafoxidine, zindoxifene, tesmilifene,miproxifene phosphate, RU 58,688, EM 139, ICI 164,384, ICI 182,780,clomiphene, MER-25, diethylstibestrol, coumestrol, genistein, GW5638,LY353581, zuclomiphene, enclomiphene, delmadinone acetate, DPPE(N,N-diethyl-2-{4-(phenylmethyl)-phenoxy}ethanamine), TSE-424, WAY-070,WAY-292, WAY-818, cyclocommunol, prinaberel, ERB-041, WAY-397, WAY-244,ERB-196, WAY-169122, MF-101, ERb-002, ERB-037, ERB-017, BE-1060, BE-380,BE-381, WAY-358, [¹⁸F]FEDNP, LSN-500307, AA-102, Ban zhi lian, CT-101,CT-102, VG-101; GnRH agonists or antagonists, such as, leuprolide,goserelin, triptorelin, alfaprostol, histrelin, detirelix, ganirelix,antide iturelix, cetrorelix, ramorelix, ganirelix, antarelix, teverelix,abarelix, ozarelix, sufugolix, prazarelix, degarelix, NBI-56418,TAK-810, acyline; FSH agonist/antagonist, LH agonist/antagonists,aromatase inhibitors, such as, letrozole, anastrazole, atamestane,fadrozole, minamestane, exemestane, plomestane, liarozole, NKS-01,vorozole, YM-511, finrozole, 4-hydroxyandrostenedione, aminogluethimide,rogletimide; steroidal or nonsteroidal glucocorticoid receptor ligands,such as, ZK-216348, ZK-243149, ZK-243185, LGD-5552, mifepristone,RPR-106541, ORG-34517, GW-215864X, sesquicillin, CP-472555, CP-394531,A-222977, AL-438, A-216054, A-276575, CP-394531, CP-409069, UGR-07;steroidal or nonsterodial progesterone receptor ligands; steroidal ornonsteroidal AR antagonists such as flutamide, hydroxyflutamide,bicalutamide, nilutamide, hydroxysteroid dehydrogenase inhibitors, PPARαligand such as bezafibrate, fenofibrate, gemfibrozil; PPARα ligands suchas darglitazone, pioglitazone, rosiglitazone, isaglitazone,rivoglitazone, netoglitazone; dual acting PPAR ligands, such asnaveglitazar, farglitazar, tesaglitazar, ragaglitazar, oxeglitazar,PN-2034, PPAR 6; an anti-glucocorticoid such as RU-486; a17-ketoreductase inhibitor, 30-DHA4,6-isomerase inhibitors,30-DHA4,5-isomerase inhibitors, 17,20-desmolase inhibitors, p450c17inhibitors, p450ssc inhibitors, 17,20-lyase inhibitors, or combinationsthereof.

In some embodiments, the compositions will further comprise ghrelinreceptor ligand or growth hormone analogues and secretagogues, IGF-1,IGF-1 analogues and secretagogues, myostatin analogues, proteasomeinhibitors, androgenic-anabolic steroids, Enbrel®, melanocortin 4receptor agonist, insulins, or combinations thereof. Such compositionsmay be used, in some embodiments, for promoting growth in feedlotanimals.

In some embodiments, the composition will comprise the compounds asdescribed herein, as well as another therapeutic compound, includinginter alia, ghrelin receptor ligand or growth hormone analogues andsecretagogues, such as, pralmorelin, examorelin, tabimorelin,capimorelin, capromorelin, ipamorelin, EP-01572, EP-1572, or JMV-1843,an androgenic anabolic steroid such as testosterone or oxandrolone, amelanocortin 4 receptor agonist, such as bremelanotide; a ghrelin oranalogue thereof, such as human ghrelin, CYT-009-GhrQb, L-692429,GHRP-6, SK&F-110679, U-75799E, leptin (metreleptin, pegylated leptin; aleptin receptor agonist, such as LEP(116-130), OB3, [D-Leu4]-OB3,rAAV-leptin, AAV-hOB, rAAVhOB; an insulin (short-, intermediate-, andlong acting formulations); a cortisol or corticosteroid, or acombination thereof.

The invention contemplates, in some embodiments, administration ofcompositions comprising the individual agents, administered separatelyand by similar or alternative routes, formulated as appropriately forthe route of administration. The invention contemplates, in someembodiments, administration of compositions comprising the individualagents, administered in the same formulation. The inventioncontemplates, in some embodiments, staggered administration, concurrentadministration, of administration of the various agents over a course oftime, however, their effects are synergistic in the subject.

It is to be understood that any of the above means, timings, routes, orcombinations thereof, of administration of two or more agents is to beconsidered as being encompassed by the phrase “administered incombination”, as described herein.

It is to be understood that reference to “a compound of this invention”or a use thereof is to be considered to encompass use of any compound asherein described, including any embodiment thereof. It is to beconsidered to encompass all of compounds which may be characterized bythe structure of formulas I-XXV.

In one embodiment, the compound is administered in combination with anagent, which treats bone diseases, disorders or conditions, such asosteoporosis, bone fractures, etc., and this invention comprises methodsof treating the same, by administering the compounds as hereindescribed, alone or in combination with other agents.

Such agents for combined use may comprise a SERM, as herein described, abisphosphonate, for example, alendronate, tiludroate, clodroniate,pamidronate, etidronate, alendronate, zolendronate, cimadronate,neridronate, minodronic acid, ibandronate, risedronate, orhomoresidronate; a calcitonin, for example, salmon, Elcatonin®,SUN-8577, TJN-135; a vitamin D or derivative (ZK-156979); a vitamin Dreceptor ligand or analogues thereof, such as calcitriol, topitriol,ZK-150123, TEI-9647, BXL-628, Ro-26-9228, BAL-2299, Ro-65-2299, orDP-035; an estrogen, estrogen derivative, or conjugated estrogen; anantiestrogen, progestin, synthetic estrogen/progestin; a RANK ligandmAb, for example, denosumab or AMG162 (Amgen); a beta 3 integrinreceptor antagonist, an osteoclast vacuolar ATPase inhibitor, anantagonist of VEGF binding to osteoclast receptors, a calcium receptorantagonist, PTh (parathyroid hormone) or analogues thereof, PTHrPanalogues (parathyroid hormone-related peptide), cathepsin K inhibitors(AAE581), strontium ranelate, tibolone, HCT-1026, PSK3471, galliummaltolate, Nutropin AQ®, prostaglandins, p38 protein kinase inhibitor, abone morphogenetic protein, an inhibitor of BMP antagonism, an HMG-CoAreductase inhibitor, a vitamin K or derivative, an antiresorptive, anipriflavone, a fluoride salt, dietary calcium supplement,osteoprotegerin, or any combination thereof. In one embodiment, thecombined administration of a SARM as herein described, osteoprotegerinand parathyroid hormone is contemplated for treating any disease,disorder or condition of the bone.

In one embodiment, the compound is administered with an agent used totreat a wasting disease. In some embodiments, agents used to treat awasting disease include but are not limited to corticosteroids, anabolicsteroids, cannabinoids, metoclopramide, cisapride, medroxyprogesteroneacetate, megestrol acetate, cyproheptadine, hydrazine sulfate,pentoxifylline, thalidomide, anticytokine antibodies, cytokineinhibitors, eicosapentaenoic acid, indomethacin, ibuprofen, melatonin,insulin, growth hormone, clenbuterol, porcine pancreas extract, IGF-1,IGF-1 analogue and secretagogue, myostatin analogue, proteasomeinhibitor, testosterone, oxandrolone, Enbrel®, melanocortin 4 receptoragonist, or a combination thereof.

In one embodiment, the agent used to treat a wasting disease is aghrelin receptor ligand, growth hormone analogue, or a secretagogue. Insome embodiments, ghrelin receptor ligands, growth hormone analogues, orsecretagogues include but are not limited to pralmorelin, examorelin,tabimorelin, capimorelin, capromorelin, ipamorelin, EP-01572, EP-1572,or JMV-1843.

In one embodiment, growth promoting agents such as but not limited toTRH, diethylstilbesterol, theophylline, enkephalins, E seriesprostaglandins, compounds disclosed in U.S. Pat. No. 3,239,345, e.g.,zeranol, and compounds disclosed in U.S. Pat. No. 4,036,979, e.g.,sulbenox or peptides disclosed in U.S. Pat. No. 4,411,890 are utilizedas agents used to treat a wasting disease.

In other embodiments, agents treating a wasting disease may comprisegrowth hormone secretagogues such as GHRP-6, GHRP-1 (as described inU.S. Pat. No. 4,411,890 and publications WO 89/07110 and WO 89/07111),GHRP-2 (as described in WO 93/04081), NN703 (Novo Nordisk), LY444711(Lilly), MK-677 (Merck), CP424391 (Pfizer) and B-HT920, or, in otherembodiments, with growth hormone releasing factor and its analogs orgrowth hormone and its analogs, or with alpha-adrenergic agonists, suchas clonidine or serotinin 5-HT_(D) agonists, such as sumatriptan, oragents which inhibit somatostatin or its release, such as physostigmineand pyridostigmine. In some embodiments, agents treating a wastingdisease may comprise parathyroid hormone, PTH (1-34) or bisphosphonates,such as MK-217 (alendronate). In other embodiments, agents treatingwasting disease may further comprise estrogen, a selective estrogenreceptor modulator, such as tamoxifen or raloxifene, or other androgenreceptor modulators, such as those disclosed in Edwards, J. P. et al.,Bio. Med. Chem. Let., 9, 1003-1008 (1999) and Hamann, L. G. et al., J.Med. Chem., 42, 210-212 (1999). In some embodiments, agents treating awasting disease may further comprise a progesterone receptor agonists(“PRA”), such as levonorgestrel, medroxyprogesterone acetate (MPA). Insome embodiments, agents treating a wasting disease may includenutritional supplements, such as those described in U.S. Pat. No.5,179,080, which, in other embodiments are in combination with wheyprotein or casein, amino acids (such as leucine, branched amino acidsand hydroxymethylbutyrate), triglycerides, vitamins (e.g., A, B6, B12,folate, C, D and E), minerals (e.g., selenium, magnesium, zinc,chromium, calcium and potassium), carnitine, lipoic acid, creatinine,β-hyroxy-β-methylbutyriate (Juven®) and coenzyme Q. In one embodiment,agents treating a wasting disease may further comprise antiresorptiveagents, vitamin D analogues, elemental calcium and calcium supplements,cathepsin K inhibitors, MMP inhibitors, vitronectin receptorantagonists, Src SH2 antagonists, vacuolar-H⁺-ATPase inhibitors,ipriflavone, fluoride, tibolone, prostanoids, 17-beta hydroxysteroiddehydrogenase inhibitors and Src kinase inhibitors.

In one embodiment, the SARM compound is administered with an agenttreating osteoporosis. In some embodiments, agents treating osteoporosisinclude but are not limited to SERMs, calcitonin, vitamin D, vitamin Dderivatives, vitamin D receptor ligand, vitamin D receptor ligandanalogue, estrogen, estrogen derivative, conjugated estrogen,antiestrogen, progestin, synthetic estrogen, synthetic progestin, RANKligand monoclonal antibody, integrin receptor antagonist, osteoclastvacuolar ATPase inhibitor, antagonist of VEGF binding to osteoclastreceptors, calcium receptor antagonist, parathyroid hormone, parathyroidhormone analogue, parathyroid hormone-related peptide, cathepsin Kinhibitor, strontium ranelate, tibolone, HCT-1026, PSK3471, galliummaltolate, Nutropin AQ®, prostaglandin, p38 protein kinase inhibitor,bone morphogenetic protein (BMP), inhibitor of BMP antagonism, HMG-CoAreductase inhibitor, vitamin K, vitamin K derivative, ipriflavone,fluoride salts, dietary calcium supplement, or osteoprotegerin.

In one embodiment, the agent treating osteoporosis is a calcitonin. Insome embodiments, calcitonins include but are not limited to salmon,Elcatonin®, SUN-8577, or TJN-135.

In one embodiment, the agent treating osteoporosis is a vitamin Dreceptor ligand or analogue. In some embodiments, vitamin D receptorligands or analogues include but are not limited to calcitriol,topitriol, ZK-150123, TEI-9647, BXL-628, Ro-26-9228, BAL-2299,Ro-65-2299, or DP-035.

In one embodiment, the compound of this invention is administered with avitamin. In some embodiments, vitamins include but are not limited tovitamin D, vitamin E, vitamin K, vitamin B, vitamin C, or a combinationthereof.

In one embodiment, this invention provides (i) a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof; (ii) a method of increasing the physical function of asubject suffering from Duchenne muscular dystrophy; (iii) a method ofincreasing the quality of life of a subject suffering from Duchennemuscular dystrophy; (iv) a method of increasing the survival of asubject suffering from Duchenne muscular dystrophy; (v) a method oftreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of cardiomyopathy in a subjectsuffering from Duchenne muscular dystrophy; (vi) a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of respiratory failure in a subject sufferingfrom Duchenne muscular dystrophy; (vii) a method of treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Becker muscular dystrophy; (viii) a method oftreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of myotonic dystrophy, limb-girdlemuscular dystrophy, facioscapulhumeral muscular dystrophy, congenitalmuscular dystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy; (ix) a method ofimproving or preserving lung function of a subject suffering fromDuchenne muscular dystrophy; (x) a method of improving cardiac functionof a subject suffering from Duchenne muscular dystrophy,

comprising the step of administering to said subject a combinationcomprising a selective androgen receptor modulator (SARM) compound and atherapeutic agent selected from the group consisting of an exon-skippinganti-sense oligonucleotide, exon skipping anti-sense molecule, utrophinup-regulator, up-regulator of other proteins that co-localize withdystrophin, myostatin inhibitor, stem cell based gene therapy, virusvectored gene therapy, non-sense codon skipping molecule, respiratorysupportive care, cardiac supportive care, anti-inflammatory agent,anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxide donor,nitric oxide precursor, nitric oxide modulator, agent that improvemitochondrial function, and/or agent that promote calcium homeostasis;

wherein said SARM compound is represented by the structure of formulaS-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, the SARM compound is administered with an agenttreating a muscular dystrophy. In some embodiments, the musculardystrophy is Duchenne muscular dystrophy. In some embodiments, themuscular dystrophy is Becker muscular dystrophy. In some embodiments,the muscular dystrophy is myotonic muscular dystrophy. Duchenne musculardystrophy is a congenital genetic disease leading to many efforts tocorrect the mutation in the dystrophin gene or compensate for the lossof dystrophin protein. Disease-modifying therapeutic strategies alongthese lines including stem cell based therapies, viral gene therapies,exon-skipping anti-sense oligomers, non-sense codon skipping molecules(a.k.a., ribosomal read through of non-sense stop codons), and utrophin(or other dystrophin co-localized protein) upregulators. However, nodisease-modifying treatments yet exist in the United States.

Stem cell based therapies for Duchenne muscular dystrophy attempt to usecells to express dystrophin. Often healthy precursor stem cell lines areused which include myoblasts, fibroblasts, bone-marrow derived stemcells, CD133+ stem cells, mesangioblasts, and more recently inducedpluripotent stem (iPS) cells. A problem with these approaches is theimmunogenicity of introducing biological materials that are foreign tothe patients. Genetically-modified patient precursor cells help withimmunogenicity but still suffer from low uptake of the cells into thetarget tissue(s) and the stability of expression of dystrophin afterintroduction.

Virus vectored gene therapies (viral gene therapies) for Duchennemuscular dystrophy attempt to express mini- or micro-dystrophin intarget tissues. Viral vectors again can cause immunogenicity and thelarge size and complexity of dystrophin limits the ability to packageits genetic material in a viral vector. The abridged dystrophinexpression may not be able to fully compensate for the congenitaldystrophin defect. Viral vectors include lentiviruses and adenovirusassociated vectors (AAV). Several recombinant AAV (rAAV) vectors havebeen tested for Duchenne muscular dystrophy including rAAV2, rAAV8,rAAV6, and rAAV9. Penetration into all target tissues, low proteinexpression levels, and stability of expression are common problems.

Exon-skipping anti-sense oligonucleotides are nucleic acids which arecomplementary to pre mRNA in the dystrophin gene and attempt to allowthe spliceosome to skip the mutated exon and express functiondystrophin. In theory, if successful, this general approach could treat−83% of Duchenne muscular dystrophy patients which have a mutation in asingle exon. However, the diversity of mutations leading to Duchennemuscular dystrophy means that the therapeutics would have to be tailoredon a patient-by-patient basis. Also, similar to other nucleotide basedtherapies, penetration into the target tissues and low and/or unstableprotein expression have been observed. Numerous nucleic acidtechnologies have been used in anti-sense approaches including2′O-methyl phosphorothiolate (2′OMeP) such as drisapersen (PRO0051),phosphorodiamidate morpholino oligomers (PMO) such as eteplirsen,tricyclo-DNA, octaguanidinium conjugated PMO and cell-penetratingpeptide conjugates (CPP). CPP approaches have included arginine-rich,Pip (PNA/PMO internalization peptides), phage and chimeric peptides.Liposomes have also been used to try to improve cell penetration.

Non-sense codon skipping molecules represent another exon skippingstrategy employing small molecules bind to the ribosome allowing theribosome to read through certain non-sense ‘stop’ codons, therebyproducing full-length and functional dystrophin. This type of mutationsuppression would only be feasible in a minority (˜15%) of Duchennemuscular dystrophy patients possessing these non-sense stop codons.Initially the aminoglycoside gentamycin and subsequently negamycin werediscovered to possess this activity however, toxicity concerns and poorefficacy plagued attempts to use these agents. A structurally unrelatedsmall molecule, ataluren, has conditional marketing authorization in theEuropean Union for treating non-sense mutation Duchenne musculardystrophy and non-sense mutation cystic fibrosis. RTC13 and RTC14 areother similar small molecule drugs in testing for Duchenne musculardystrophy.

Utrophin upregulators are often small molecules but also biologics thatupregulate utrophin, a protein which has been observed to partiallycompensate for the loss of dystrophin. For example, the NSAID nabumetonewas discovered in a high-throughput screen to up-regulate utrophin.Other small molecule utrophin upregulators such as SMT C1100 andSMT022357activate the utrophin-A promoter. UtroUp is a novel six zincfinger artificial transcription factor that recognizes 18 base pairs ofthe utrophin promoter and efficiently drives utrophin upregulation.Heregulin is another protein which upregulates utrophin. Recombinanthuman biglycan post-translationally ‘upregulates’ utrophin activity byrecruiting it to the sarcolemma. Gene therapy approaches to increaseexpression of utrophin are also in testing. Also some are pursuing theupregulation of other proteins that co-localize with dystrophin, andhence can partially compensate for the loss of dystrophin (similar toutrophin).

Although there are many efforts to correct or compensate for the lack ofdystrophin, currently no disease modifying drugs are approved in theUnited States. The extreme complexity of the dystrophin gene (large genewith a relatively high number of exons requiring complex splicing toproduce the protein), the vast number of mutations leading to Duchennemuscular dystrophy (up to ⅓ of cases represent new mutations of the DMDgene), and the inherent problems of nucleotide or protein basedtherapeutics (i.e., cell penetration in all target tissues, maintainingexpression in target tissues, high doses required, immunogenicity ofvectors or expressed proteins, etc.) complicates the design of suchtherapies.

The inherent difficulties above suggest that symptom-directed treatmentsare needed. Current treatments are directed at supportive care thatprotects the target organs involved in the terminal phase of thedisease, i.e., cardiac and respiratory insufficiencies eventuallyleading to death. Respiratory supportive care includes anti-inflammatoryglucocorticoids such as prednisone or deflazacort and non-invasivepositive pressure ventilation (NIPPV); whereas cardiac supportive careincludes angiotensin converting enzyme inhibitors (ACEIs), angiotensinII receptor blockers (ARBs), beta-adrenergic receptor blockers,aldosterone receptor blockers such as spironolactone, and diuretics.Copolymer poloxamers (e.g., copolymer poloxamer P188) are also intesting for cardiac supportive care.

Although symptom-directed therapies do not increase the levels ofdystrophin or related proteins expressed in Duchenne muscular dystrophypatients, they do improve quality of life and survival times. Advancingknowledge of the pathophysiology has led to many classes of emergingtherapeutics that are directed toward symptomatic treatment includinganti-inflammatory agents, anti-fibrotic agents, anti-oxidants, anabolicagents, nitric oxide (NO) donors, NO precursors, NO modulators, agentsto improve mitochondrial function, and agents that promote calciumhomeostasis.

Anti-inflammatory agents in use or testing for Duchenne musculardystrophy include non-specific anti-inflammatory agents such asprednisone and deflazacort, but also a variety of targetedanti-inflammatory agents such as infliximab, etanercept, NEMO bindingdomain (NBD) peptides, AAV-p65-shRNA, curcumin, genistein, NSAIDs suchas ibuprofen, cromolyn, TLR 7/9 antagonists, and pyrrollidinedithiocarbamate. Reduction of inflammation slows the deterioration ofmuscles and their conversion to fibrous and fatty tissues.

Anti-fibrotic agents in testing for Duchenne muscular dystrophy includeACEIs such as lisinopril, ARBs such as losartan, halofunginone,pirfenidone, suramin, imatinib mesylate, and miRNA-29. Dystrophicmuscles stain as predominantly fibrotic and the extent of fibrosiscorrelates well clinically with the severity of cardiac and pulmonaryinsufficiencies. The ability to prevent or reverse fibrosis of skeletal,cardiac or smooth muscles would delay deterioration of patients and/orimprove quality of life.

Inflammation, fibrosis, mitochondrial deregulation, membrane fragility,etc. each contribute to the development of intracellular oxidativestress. Anti-oxidants in testing for Duchenne muscular dystrophy includeN-acetyl cysteine (NAC), epigallocatechin gallate (EGCG or green teaextract), melatonin, idebenone (a short chain benoquinone), andpentoxifylline.

Agents to improve mitochondrial function in testing for Duchennemuscular dystrophy include AMPK activators such as AICAR and metformin;GW50156, and cyclophilin D inhibitors such as Debio-025.

Agents to promote calcium homeostasis that are in testing for Duchennemuscular dystrophy include imipramine and amitriptyline.

Nitric oxide (NO) donors, precursors and modulators in testing forDuchenne muscular dystrophy include phosphodiesterase type 5A (PDE5A)inhibitors such as sildenafil and tadalafil, nitric oxide precursorssuch a L-arginine and L-citrulline, cyclo-oxygenase (COX) inhibitorynitric oxide donors (CINODs) such as naproxcinod (HCT3012) and HCT 1026,and nitric oxide donors such as isosorbide dinitrate and sodium nitrate.NO is a potent vasodilator in the vasculature including that supplyingthe muscles. The neuronal nitric oxide synthase (nNOS) system isderegulated in dystrophic muscles contributing to their poor vascularsupply and deterioration.

The end result of the various pathologies above weakens and atrophiesskeletal, cardiac and smooth muscle (i.e., muscle wasting) resulting infunctional deficits and cardiac and respiratory insufficiencies.Anabolic agents promote the growth of muscles and thereby counteractmuscle wasting. Anabolic agents in testing for Duchenne musculardystrophy include myostatin inhibition such as MYO-029 (recombinanthuman inhibitory antibody that binds myostatin), ACE-031 (a solubleactivin type IIB receptor), and follistatin (a myostatin bindingprotein). Other anabolics include insulin-like growth factor-1(Increlex), SARMs such as GLPG-0492, steroidal androgens such asoxandrolone, and testosterone. Other muscle supportive therapies includeHMG-CoA reductase inhibitors such as simvastatin or creatinesupplementation.

In some embodiments, any of the compositions of this invention willcomprise a compound of formula I-XXV, in any form or embodiment asdescribed herein. In some embodiments, any of the compositions of thisinvention will consist of a compound of formula I-XXV, in any form orembodiment as described herein. In some embodiments, of the compositionsof this invention will consist essentially of a compound of I-XXV, inany form or embodiment as described herein. In some embodiments, theterm “comprise” refers to the inclusion of the indicated active agent,such as the compound of formula I-XXV, as well as inclusion of otheractive agents, and pharmaceutically acceptable carriers, excipients,emollients, stabilizers, etc., as are known in the pharmaceuticalindustry. In some embodiments, the term “consisting essentially of”refers to a composition, whose only active ingredient is the indicatedactive ingredient, however, other compounds may be included which arefor stabilizing, preserving, etc. the formulation, but are not involveddirectly in the therapeutic effect of the indicated active ingredient.In some embodiments, the term “consisting essentially of” may refer tocomponents which facilitate the release of the active ingredient. Insome embodiments, the term “consisting” refers to a composition, whichcontains the active ingredient and a pharmaceutically acceptable carrieror excipient.

In one embodiment, the present invention provides combined preparations.In one embodiment, the term “a combined preparation” defines especiallya “kit of parts” in the sense that the combination partners as definedabove can be dosed independently or by use of different fixedcombinations with distinguished amounts of the combination partnersi.e., simultaneously, concurrently, separately or sequentially. In someembodiments, the parts of the kit of parts can then, e.g., beadministered simultaneously or chronologically staggered, that is atdifferent time points and with equal or different time intervals for anypart of the kit of parts. The ratio of the total amounts of thecombination partners, in some embodiments, can be administered in thecombined preparation. In one embodiment, the combined preparation can bevaried, e.g., in order to cope with the needs of a patient subpopulationto be treated or the needs of the single patient which different needscan be due to a particular disease, severity of a disease, age, sex, orbody weight as can be readily made by a person skilled in the art.

It is to be understood that this invention is directed to compositionsand combined therapies as described herein, for any disease, disorder orcondition, as appropriate, as will be appreciated by one skilled in theart. Certain applications of such compositions and combined therapieshave been described hereinabove, for specific diseases, disorders andconditions, representing embodiments of this invention, and methods oftreating such diseases, disorders and conditions in a subject byadministering a compound as herein described, alone or as part of thecombined therapy or using the compositions of this invention representadditional embodiments of this invention.

Biological Activity of Selective Androgen Modulator Compounds

In some embodiments, the compounds of this invention possess in vivotissue selective androgenic and anabolic activity, which is accordinglyutilized for particular applications, as will be appreciated by oneskilled in the art.

In one embodiment, the methods of this invention are useful a subject,which is a human. In another embodiment, the subject is a mammal. Inanother embodiment the subject is an animal. In another embodiment thesubject is an invertebrate. In another embodiment the subject is avertebrate. In one embodiment, the subject is a feedlot animal, a beefcattle and/or a finishing livestock.

In one embodiment, the subject is male. In another embodiment, thesubject is female. In some embodiments, while the methods as describedherein may be useful for treating either males or females, females mayrespond more advantageously to administration of certain compounds, forcertain methods, as described and exemplified herein.

In another embodiment of the present invention, a method is provided forhormonal therapy in a patient (i.e., one suffering from anandrogen-dependent condition) which includes contacting an androgenreceptor of a patient with a compound and/or a non steroidal agonist ofthe present invention and/or its analog, derivative, isomer, metabolite,pharmaceutically acceptable salt, pharmaceutical product, polymorph,crystal, impurity, hydrate, N-oxide or any combination thereof, in anamount effective to bind the compound to the androgen receptor andeffect a change in an androgen-dependent condition.

In one embodiment of this invention, a method is provided for hormonereplacement therapy in a patient (i.e., one suffering from anandrogen-dependent condition) which includes administering a compound asherein described and/or its analog, derivative, isomer, metabolite,pharmaceutically acceptable salt, pharmaceutical product, polymorph,crystal, impurity, hydrate, N-oxide or any combination thereof, to asubject, in an amount sufficient to effect a change in ahormone-dependent condition in the subject.

In one embodiment, this invention provides for the use of a compound asherein described, or its prodrug, analog, isomer, metabolite,derivative, pharmaceutically acceptable salt, pharmaceutical product,polymorph, crystal, impurity, N-oxide, hydrate or any combinationthereof, for: a) accelerating bone repair; b) treating bone disorders;c) treating bone density loss; d) treating low bone mineral density(BMD); e) treating reduced bone mass; f) treating metabolic bonedisease; g) promoting bone growth or regrowth; h) promoting bonerestoration; i) promoting bone fracture repair; j) promoting boneremodeling; k) treating bone damage following reconstructive surgeryincluding of the face, hip, or joints; l) enhancing of bone strength andfunction; m) increasing cortical bone mass; or n) increasing trabecularconnectivity.

In one embodiment, the bone related disorder is a genetic disorder, orin another embodiment, is induced as a result of a treatment regimen fora given disease. For example, and in one embodiment, the compounds asherein described are useful in treating a bone-related disorder thatarises as a result of cancer metastasis to bone, or in anotherembodiment, as a result of androgen-deprivation therapy, for example,given in response to prostate carcinogenesis in the subject.

In one embodiment, the bone-related disorder is osteoporosis. In anotherembodiment, the bone-related disorder is osteopenia. In anotherembodiment, the bone-related disorder is increased bone resorption. Inanother embodiment, the bone-related disorder is bone fracture. Inanother embodiment, the bone-related disorder is bone frailty.

In another embodiment, the bone-related disorder is a loss of bonemineral density (BMD). In another embodiment, the bone-related disorderis any combination of osteoporosis, osteopenia, increased boneresorption, bone fracture, bone frailty and loss of BMD. Each disorderrepresents a separate embodiment of the present invention.

“Osteoporosis” refers, in one embodiment, to a thinning of the boneswith reduction in bone mass due to depletion of calcium and boneprotein. In another embodiment, osteoporosis is a systemic skeletaldisease, characterized by low bone mass and deterioration of bonetissue, with a consequent increase in bone fragility and susceptibilityto fracture. In osteoporotic patients, bone strength is abnormal, in oneembodiment, with a resulting increase in the risk of fracture. Inanother embodiment, osteoporosis depletes both the calcium and theprotein collagen normally found in the bone, in one embodiment,resulting in either abnormal bone quality or decreased bone density. Inanother embodiment, bones that are affected by osteoporosis can fracturewith only a minor fall or injury that normally would not cause a bonefracture. The fracture can be, in one embodiment, either in the form ofcracking (as in a hip fracture) or collapsing (as in a compressionfracture of the spine). The spine, hips, and wrists are common areas ofosteoporosis-induced bone fractures, although fractures can also occurin other skeletal areas. Unchecked osteoporosis can lead, in anotherembodiment, to changes in posture, physical abnormality, and decreasedmobility.

In one embodiment, the osteoporosis results from androgen deprivation.In another embodiment, the osteoporosis follows androgen deprivation. Inanother embodiment, the osteoporosis is primary osteoporosis. In anotherembodiment, the osteoporosis is secondary osteoporosis. In anotherembodiment, the osteoporosis is postmenopausal osteoporosis. In anotherembodiment, the osteoporosis is juvenile osteoporosis. In anotherembodiment, the osteoporosis is idiopathic osteoporosis. In anotherembodiment, the osteoporosis is senile osteoporosis.

In one embodiment, the methods of this invention are useful in treatingdiseases or disorders caused by, or associated with a hormonal disorder,disruption or imbalance. In one embodiment, the hormonal disorder,disruption or imbalance comprises an excess of a hormone. In anotherembodiment, the hormonal disorder, disruption or imbalance comprises adeficiency of a hormone. In one embodiment, the hormone is a steroidhormone. In another embodiment, the hormone is an estrogen. In anotherembodiment, the hormone is an androgen. In another embodiment, thehormone is a glucocorticoid. In another embodiment, the hormone is acortico-steroid. In another embodiment, the hormone is luteinizinghormone (LH). In another embodiment, the hormone is follicle stimulatinghormone (FSH). In another embodiment, the hormone is any other hormoneknown in the art. In another embodiment, the hormonal disorder,disruption or imbalance is associated with menopause. In anotherembodiment, the hormonal disorder, disruption or imbalance is associatedwith andropause, andropausal vasomotor symptoms, andropausalgynecomastia, muscle strength and/or function, bone strength and/orfunction and anger. In another embodiment, hormone deficiency is aresult of specific manipulation, as a byproduct of treating a disease ordisorder in the subject. For example, the hormone deficiency may be aresult of androgen depletion in a subject, as a therapy for prostatecancer in the subject. Each possibility represents a separate embodimentof the present invention.

In another embodiment the invention is directed to treating sarcopeniaor cachexia, and associated conditions related thereto, for examplediseases or disorders of the bone.

In one embodiment, this invention provides for the use of a compound asherein described, or its prodrug, analog, isomer, metabolite,derivative, pharmaceutically acceptable salt, pharmaceutical product,polymorph, crystal, impurity, N-oxide, hydrate or any combinationthereof, for: 1) treating a muscle wasting disorder; 2) preventing amuscle wasting disorder; 3) treating, preventing, suppressing,inhibiting or reducing muscle loss due to a muscle wasting disorder; 4)treating, preventing, inhibiting, reducing or suppressing muscle wastingdue to a muscle wasting disorder; 5) treating, preventing, inhibiting,reducing or suppressing muscle protein catabolism due to a musclewasting disorder; 6) treating, preventing, inhibiting, reducing orsuppressing end stage renal disease; and/or 7) treating, preventing,inhibiting, reducing or suppressing frailty.

In another embodiment, the use of a compound for treating a subjecthaving a muscle wasting disorder, or any of the disorders describedherein, includes administering a pharmaceutical composition including acompound as herein described. In another embodiment, the administeringstep includes intravenously, intraarterially, or intramuscularlyinjecting to said subject said pharmaceutical composition in liquidform; subcutaneously implanting in said subject a pellet containing saidpharmaceutical composition; orally administering to said subject saidpharmaceutical composition in a liquid or solid form; or topicallyapplying to the skin surface of said subject said pharmaceuticalcomposition.

A muscle is a tissue of the body that primarily functions as a source ofpower. There are three types of muscles in the body: a) skeletalmuscle—the muscle responsible for moving extremities and external areasof the bodies; b) cardiac muscle—the heart muscle; and c) smoothmuscle—the muscle that is in the walls of arteries and bowel.

A wasting condition or disorder is defined herein as a condition ordisorder that is characterized, at least in part, by an abnormal,progressive loss of body, organ or tissue mass. A wasting condition canoccur as a result of a pathology such as, for example, cancer, or aninfection, or it can be due to a physiologic or metabolic state, such asdisuse deconditioning that can occur, for example, due to prolonged bedrest or when a limb is immobilized, such as in a cast. A wastingcondition can also be age associated. The loss of body mass that occursduring a wasting condition can be characterized by a loss of total bodyweight, or a loss of organ weight such as a loss of bone or muscle massdue to a decrease in tissue protein.

In one embodiment, “muscle wasting” or “muscular wasting”, used hereininterchangeably, refer to the progressive loss of muscle mass and/or tothe progressive weakening and degeneration of muscles, including theskeletal or voluntary muscles which control movement, cardiac muscleswhich control the heart, and smooth muscles. In one embodiment, themuscle wasting condition or disorder is a chronic muscle wastingcondition or disorder. “Chronic muscle wasting” is defined herein as thechronic (i.e. persisting over a long period of time) progressive loss ofmuscle mass and/or to the chronic progressive weakening and degenerationof muscle.

The loss of muscle mass that occurs during muscle wasting can becharacterized by a muscle protein breakdown or degradation, by muscleprotein catabolism. Protein catabolism occurs because of an unusuallyhigh rate of protein degradation, an unusually low rate of proteinsynthesis, or a combination of both. Protein catabolism or depletion,whether caused by a high degree of protein degradation or a low degreeof protein synthesis, leads to a decrease in muscle mass and to musclewasting. The term “catabolism” has its commonly known meaning in theart, specifically an energy burning form of metabolism.

Muscle wasting can occur as a result of a pathology, disease, conditionor disorder. In one embodiment, the pathology, illness, disease orcondition is chronic. In another embodiment, the pathology, illness,disease or condition is genetic. In another embodiment, the pathology,illness, disease or condition is neurological. In another embodiment,the pathology, illness, disease or condition is infectious. As describedherein, the pathologies, diseases, conditions or disorders for which thecompounds and compositions of the present invention are administered arethose that directly or indirectly produce a wasting (i.e. loss) ofmuscle mass, that is a muscle wasting disorder.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof, comprising the step of administering a pharmaceuticalcomposition comprising a selective androgen receptor modulator (SARM)compound represented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof; and a pharmaceutically acceptable carrier.

In one embodiment, the present invention is directed to a method oftreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of Duchenne muscular dystrophy in asubject in need thereof, comprising the step of administering to saidsubject a combination comprising a selective androgen receptor modulator(SARM) compound and a therapeutic agent selected from the groupconsisting of an exon-skipping anti-sense oligonucleotide, exon skippinganti-sense molecule, utrophin up-regulator, up-regulator of otherproteins that co-localize with dystrophin, myostatin inhibitor, stemcell based gene therapy, virus vectored gene therapy, non-sense codonskipping molecule, respiratory supportive care, cardiac supportive care,anti-inflammatory agent, anti-fibrotic agent, anti-oxidant, anabolicagent, nitric oxide donor, nitric oxide precursor, nitric oxidemodulator, agent that improve mitochondrial function, and/or agent thatpromote calcium homeostasis, wherein said SARM compound is representedby the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, the combination is a combination of the SARM compoundand an exon-skipping oligonucleotide. In one embodiment, the combinationis a combination of the SARM compound and exon skipping anti-sensemolecule. In one embodiment, the combination is a combination of theSARM compound and an utrophin up-regulator. In one embodiment, thecombination is a combination of the SARM compound and an up-regulator ofother proteins that co-localize with dystrophin. In another embodiment,the combination is a combination of the SARM compound and a myostatininhibitor. In one embodiment, the combination is a combination of theSARM compound and stem cell based gene therapy. In one embodiment, thecombination is a combination of the SARM compound and a virus vectoredgene therapy. In one embodiment, the combination is a combination of theSARM compound and a non-sense codon skipping molecule. In oneembodiment, the combination is a combination of the SARM compound and arespiratory supportive care agent. In one embodiment, the combination isa combination of the SARM compound and cardiac supportive care agent. Inone embodiment, the combination is a combination of the SARM compoundand an anti-inflammatory agent. In one embodiment, the combination is acombination of the SARM compound and an anti-fibrotic agent. In oneembodiment, the combination is a combination of the SARM compound and ananti-oxidant agent. In one embodiment, the combination is a combinationof the SARM compound and an anabolic agent. In one embodiment, thecombination is a combination of the SARM compound and a nitric oxidedonor. In one embodiment, the combination is a combination of the SARMcompound and a nitric oxide precursor. In one embodiment, thecombination is a combination of the SARM compound and a nitric oxidemodulator. In one embodiment, the combination is a combination of theSARM compound and an agent that improves mitochondrial function. In oneembodiment, the combination is a combination of the SARM compound and anagent that promotes calcium homeostasis. In one embodiment, thecombination is a combination of the SARM compound and more than one ofthe above other Duchenne muscular dystrophy treatments.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof, further increases the quality of life of said subject. Inanother embodiment, the method of this invention for treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further increases the survival of said subject. In anotherembodiment, the method of this invention for treating, reducing theseverity, reducing the incidence, delaying the onset, or reducing thepathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further increases the physical function of said subject. Inanother embodiment, the method of this invention for treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays the loss of body weight of said subject. Inanother embodiment, the method of this invention for treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays loss of ambulation of said subject. In anotherembodiment, the method of this invention for treating, reducing theseverity, reducing the incidence, delaying the onset, or reducing thepathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays loss of lean body mass of said subject. Inanother embodiment, the method of this invention for treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays the gain of fat body mass of said subject. Inanother embodiment, the method of this invention for treating, reducingthe severity, reducing the incidence, delaying the onset, or reducingthe pathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays muscle fibrosis of said subject. In anotherembodiment, the method of this invention for treating, reducing theseverity, reducing the incidence, delaying the onset, or reducing thepathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays cardiomyopathy of said subject. In anotherembodiment, the method of this invention for treating, reducing theseverity, reducing the incidence, delaying the onset, or reducing thepathogenesis of Duchenne muscular dystrophy in a subject in needthereof, further delays respiratory failure or insufficiency of saidsubject. In another embodiment, the method of this invention fortreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of Duchenne muscular dystrophy in asubject in need thereof, further increases the exercise tolerance ofsaid subject. In another embodiment, the method of this invention fortreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of Duchenne muscular dystrophy in asubject in need thereof, further decreases the extent and severity ofmuscle contractures of said subject. In another embodiment, the methodof this invention for treating, reducing the severity, reducing theincidence, delaying the onset, or reducing the pathogenesis of Duchennemuscular dystrophy in a subject in need thereof, further prevents ordelays scoliosis of said subject.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Duchenne muscular dystrophy in a subject inneed thereof, further delays onset or improves symptoms ofcardiomyopathy and/or respiratory function.

In one embodiment, this invention is directed to a method of increasingthe physical function of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of increasingthe physical function of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis,

wherein said SARM compound is represented by the structure of formulaS-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of increasingthe quality of life of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of increasingthe quality of life of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis,

wherein said SARM compound is represented by the structure of formulaS-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

The term “quality of life” refers herein to improvement of one or moreof the following: motor skills such as ambulation and limb strength,less fatigue; delaying onset, treating, or preventing cardiopathies;delaying onset, treating, or preventing respiratory symptoms andrespiratory insufficiency or failure; or improved cognition.

In one embodiment, this invention is directed to a method of increasingthe survival of a subject suffering from Duchenne muscular dystrophy,comprising the step of administering to said subject a selectiveandrogen receptor modulator (SARM) compound represented by the structureof formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of increasingthe survival of a subject suffering from Duchenne muscular dystrophy,comprising the step of administering to said subject a combinationcomprising a selective androgen receptor modulator (SARM) compound and atherapeutic agent selected from the group consisting of an exon-skippinganti-sense oligonucleotide, exon skipping anti-sense molecule, utrophinup-regulator, up-regulator of other proteins that co-localize withdystrophin, myostatin inhibitor, stem cell based gene therapy, virusvectored gene therapy, non-sense codon skipping molecule, respiratorysupportive care, cardiac supportive care, anti-inflammatory agent,anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxide donor,nitric oxide precursor, nitric oxide modulator, agent that improvemitochondrial function, and/or agent that promote calcium homeostasis,wherein said SARM compound is represented by the structure of formulaS-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiomyopathy in a subject suffering fromDuchenne muscular dystrophy, comprising the step of administering tosaid subject a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiomyopathy in a subject suffering fromDuchenne muscular dystrophy, comprising the step of administering tosaid subject a combination comprising a selective androgen receptormodulator (SARM) compound and a therapeutic agent selected from thegroup consisting of an an exon-skipping anti-sense oligonucleotide, exonskipping anti-sense molecule, utrophin up-regulator, up-regulator ofother proteins that co-localize with dystrophin, myostatin inhibitor,stem cell based gene therapy, virus vectored gene therapy, non-sensecodon skipping molecule, respiratory supportive care, cardiac supportivecare, anti-inflammatory agent, anti-fibrotic agent, anti-oxidant,anabolic agent, nitric oxide donor, nitric oxide precursor, nitric oxidemodulator, agent that improve mitochondrial function, and/or agent thatpromote calcium homeostasis, wherein said SARM compound is representedby the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiomyopathy in a subject suffering fromDuchenne muscular dystrophy, further increases the quality of life ofsaid subject. In another embodiment, the method of this invention fortreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of cardiomyopathy in a subjectsuffering from Duchenne muscular dystrophy, further increases thesurvival of said subject. In another embodiment, the method of thisinvention for treating, reducing the severity, reducing the incidence,delaying the onset, or reducing the pathogenesis of cardiomyopathy asubject suffering from Duchenne muscular dystrophy, further increasesthe physical function of said subject.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of respiratory failure in a subject sufferingfrom Duchenne muscular dystrophy, comprising the step of administeringto said subject a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of respiratory failure in a subject sufferingfrom Duchenne muscular dystrophy, comprising the step of administeringto said subject a combination comprising a selective androgen receptormodulator (SARM) compound and a therapeutic agent selected from thegroup consisting of an exon-skipping anti-sense oligonucleotide, exonskipping anti-sense molecule, utrophin up-regulator, up-regulator ofother proteins that co-localize with dystrophin, myostatin inhibitor,stem cell based gene therapy, virus vectored gene therapy, non-sensecodon skipping molecule, respiratory supportive care, cardiac supportivecare, anti-inflammatory agent, anti-fibrotic agent, anti-oxidant,anabolic agent, nitric oxide donor, nitric oxide precursor, nitric oxidemodulator, agent that improve mitochondrial function, and/or agent thatpromote calcium homeostasis, wherein said SARM compound is representedby the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of respiratory failure in a subject sufferingfrom Duchenne muscular dystrophy, further increases the quality of lifeof said subject. In another embodiment, the method of this invention fortreating, reducing the severity, reducing the incidence, delaying theonset, or reducing the pathogenesis of respiratory failure in a subjectsuffering from Duchenne muscular dystrophy, further increases thesurvival of said subject. In another embodiment, the method of thisinvention for treating, reducing the severity, reducing the incidence,delaying the onset, or reducing the pathogenesis of respiratory failurein a subject suffering from Duchenne muscular dystrophy, furtherincreases the physical function of said subject.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Becker muscular dystrophy or myotonicdystrophy in a subject in need thereof, comprising the step ofadministering to said subject a selective androgen receptor modulator(SARM) compound represented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Becker muscular dystrophy or myotonicdystrophy in a subject in need thereof, comprising the step ofadministering to said subject a combination comprising a selectiveandrogen receptor modulator (SARM) compound and a therapeutic agentselected from the group consisting of an exon-skipping anti-senseoligonucleotide, exon skipping anti-sense molecule, utrophinup-regulator, up-regulator of other proteins that co-localize withdystrophin, myostatin inhibitor, stem cell based gene therapy, virusvectored gene therapy, non-sense codon skipping molecule, respiratorysupportive care, cardiac supportive care, anti-inflammatory agent,anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxide donor,nitric oxide precursor, nitric oxide modulator, agent that improvemitochondrial function, and/or agent that promote calcium homeostasis,wherein said SARM compound is represented by the structure of formulaS-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Becker muscular dystrophy in a subject inneed thereof, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis, wherein said SARM compound is represented by the structureof formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Becker muscular dystrophy or myotonicdystrophy in a subject in need thereof, further increases the quality oflife of said subject. In another embodiment, the method of thisinvention for treating, reducing the severity, reducing the incidence,delaying the onset, or reducing the pathogenesis of Becker musculardystrophy or myotonic dystrophy in a subject in need thereof, furtherincreases the survival of said subject. In another embodiment, themethod of this invention for treating, reducing the severity, reducingthe incidence, delaying the onset, or reducing the pathogenesis ofBecker muscular dystrophy or myotonic dystrophy in a subject in needthereof, further increases the physical function of said subject.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Becker muscular dystrophy or myotonicdystrophy in a subject in need thereof, further delays onset or improvessymptoms of cardiomyopathy and/or respiratory function.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis;

-   -   wherein said SARM compound is represented by the structure of        formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy in a subject in needthereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of limb-girdle muscular dystrophy in a subjectin need thereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of facioscapulhumeral muscular dystrophy in asubject in need thereof, comprising the step of administering to saidsubject a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of congenital muscular dystrophy in a subjectin need thereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of oculopharyngeal muscular dystrophy in asubject in need thereof, comprising the step of administering to saidsubject a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of distal muscular dystrophy in a subject inneed thereof, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, this invention is directed to a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of Emery-Dreifuss muscular dystrophy in asubject in need thereof, comprising the step of administering to saidsubject a selective androgen receptor modulator (SARM) compoundrepresented by the structure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, further increases the quality of life of said subject.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, further increases the survival of said subject. In anotherembodiment, the method of this invention for treating, reducing theseverity, reducing the incidence, delaying the onset, or reducing thepathogenesis of myotonic dystrophy, limb-girdle muscular dystrophy,facioscapulhumeral muscular dystrophy, congenital muscular dystrophy,oculopharyngeal muscular dystrophy, distal muscular dystrophy, orEmery-Dreifuss muscular dystrophy in a subject in need thereof, furtherincreases the physical function of said subject.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, further delays onset or improves symptoms of cardiomyopathyand/or respiratory function.

In one embodiment, this invention provides a method of treating,reducing the incidence of, delaying progression of, reducing theseverity of, or alleviating symptoms associated with a muscle wastingdisorder in a subject, comprising the step of administering to saidsubject the selective androgen receptor modulator compound of thisinvention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, hydrate, N-oxide or any combinationthereof, or a composition comprising the same, in an amount effective totreat the muscle wasting disorder in said subject. In anotherembodiment, the compound is a compound of formula S-XXIII.

According to this aspect, and in one embodiment, the muscle wastingdisorder is due to a pathology, illness, disease or condition. In oneembodiment, the pathology, illness, disease or condition isneurological, infectious, chronic or genetic. In one embodiment, thepathology, illness, disease or condition is a muscular dystrophy, amuscular atrophy, X-linked spinal-bulbar muscular atrophy (SBMA), acachexia, malnutrition, leprosy, diabetes, renal disease, chronicobstructive pulmonary disease (COPD), cancer, end stage renal failure,sarcopenia, emphysema, osteomalacia, HIV infection, AIDS, orcardiomyopathy. In one embodiment, the compound is a compound of thisinvention. In another embodiment, the compound is a compound of formulaS-XXIII.

In one embodiment, the muscle wasting disorder is an age-associatedmuscle wasting disorder; a disuse deconditioning-associated musclewasting disorder; or the muscle wasting disorder is due to chronic lowerback pain, burns, central nervous system (CNS) injury or damage,peripheral nerve injury or damage, spinal cord injury or damage,chemical injury or damage, or alcoholism.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiovascular disease in a human subject,comprising the step of administering an effective amount of a compoundof this invention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to said subject. In another embodiment, the compound is acompound of formula S-XXIII.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiovascular disease in a human subjectsuffering from Duchenne muscular dystrophy comprising the step ofadministering an effective amount of a compound of this invention or itsisomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to said subject. Inanother embodiment, the compound is a compound of formula S-XXIII.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiovascular disease in a human subjectsuffering from Becker muscular dystrophy comprising the step ofadministering an effective amount of a compound of this invention or itsisomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to said subject. Inanother embodiment, the compound is a compound of formula S-XXIII.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cardiovascular disease in a human subjectsuffering from myotonic dystrophy comprising the step of administeringan effective amount of a compound of this invention or its isomer,pharmaceutically acceptable salt, pharmaceutical product, crystal,N-oxide, hydrate or any combination thereof to said subject. In anotherembodiment, the compound is a compound of formula S-XXIII.

In one embodiment, this invention provides a method of treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of cachexia in a subject, comprising the stepof administering an effective amount of a compound of this invention orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to said subject. Inone embodiment, the compound is of formula S-XXIII.

In one embodiment, the present invention provides a method of reducing afat mass in a subject comprising the step of administering an effectiveamount of a compound of this invention or its isomer, pharmaceuticallyacceptable salt, pharmaceutical product, crystal, N-oxide, hydrate orany combination thereof to the subject. In another embodiment, thecompound is a compound of formula S-XXIII.

In one embodiment, the present invention provides a method of reducing afat mass in a subject suffering from Duchenne muscular dystrophycomprising the step of administering an effective amount of a compoundof this invention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII. In one embodiment, the present inventionprovides a method of reducing or preventing fibrosis in a subjectsuffering from Duchenne muscular dystrophy comprising the step ofadministering an effective amount of a compound of formula S-XXIII orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to the subject.

In one embodiment, the present invention provides a method of reducing afat mass in a subject suffering from Becker muscular dystrophycomprising the step of administering an effective amount of a compoundof this invention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII. In one embodiment, the present inventionprovides a method of reducing or preventing fibrosis in a subjectsuffering from Becker muscular dystrophy comprising the step ofadministering an effective amount of a compound of formula S-XXIII orits isomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to the subject.

In one embodiment, the present invention provides a method of reducing afat mass in a subject suffering from myotonic dystrophy comprising thestep of administering an effective amount of a compound of thisinvention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII. In one embodiment, the present inventionprovides a method of reducing or preventing fibrosis in a subjectsuffering from myotonic dystrophy comprising the step of administeringan effective amount of a compound of formula S-XXIII or its isomer,pharmaceutically acceptable salt, pharmaceutical product, crystal,N-oxide, hydrate or any combination thereof to the subject.

In one embodiment, the present invention provides a method of increasinga lean mass in a subject comprising the step of administering aneffective amount of a compound of this invention or its isomer,pharmaceutically acceptable salt, pharmaceutical product, crystal,N-oxide, hydrate or any combination thereof to the subject. In anotherembodiment, the compound is a compound of formula S-XXIII.

In one embodiment, the present invention provides a method of increasinga lean mass in a subject suffering from Duchenne muscular dystrophycomprising the step of administering an effective amount of a compoundof this invention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII.

In one embodiment, the present invention provides a method of increasinga lean mass in a subject suffering from Becker muscular dystrophycomprising the step of administering an effective amount of a compoundof this invention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII.

In one embodiment, the present invention provides a method of increasinga lean mass in a subject suffering from myotonic dystrophy comprisingthe step of administering an effective amount of a compound of thisinvention or its isomer, pharmaceutically acceptable salt,pharmaceutical product, crystal, N-oxide, hydrate or any combinationthereof to the subject. In another embodiment, the compound is acompound of formula S-XXIII.

In another embodiment, this invention provides a method of treating,reducing the incidence of, delaying progression of, reducing theseverity of, or alleviating symptoms associated with a muscle wastingdisorder; reducing a fat mass; reducing or preventing fibrosis; orincreasing a lean mass in a subject, comprising the step ofadministering an effective amount of a compound of this invention or itsisomer, pharmaceutically acceptable salt, pharmaceutical product,crystal, N-oxide, hydrate or any combination thereof to the subject asherein described. In another embodiment, the compound is a compound offormula S-XXIII.

In one embodiment, muscle wasting in a subject is a result of thesubject having a muscular dystrophy; muscle atrophy; or X-linkedspinal-bulbar muscular atrophy (SBMA).

In another embodiment, the invention provides a method of improving orpreserving lung function of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In another embodiment, the method of this invention for treating,reducing the severity, reducing the incidence, delaying the onset, orreducing the pathogenesis of myotonic dystrophy, limb-girdle musculardystrophy, facioscapulhumeral muscular dystrophy, congenital musculardystrophy, oculopharyngeal muscular dystrophy, distal musculardystrophy, or Emery-Dreifuss muscular dystrophy in a subject in needthereof, further delays onset or improves symptoms of cardiomyopathyand/or respiratory function.

In one embodiment, the administering in the invention for improving orpreserving lung function comprises administering a pharmaceuticalcomposition comprising the compound represented by the structure offormula S-XXIII and/or its isomer, pharmaceutically acceptable salt,hydrate, N-oxide, or any combination thereof; and a pharmaceuticallyacceptable carrier.

In another embodiment, the invention provides a method of improving orpreserving lung function of a subject suffering from Duchenne musculardystrophy, comprising the step of administering to said subject acombination comprising a selective androgen receptor modulator (SARM)compound and a therapeutic agent selected from the group consisting ofan exon-skipping anti-sense oligonucleotide, exon skipping anti-sensemolecule, utrophin up-regulator, up-regulator of other proteins thatco-localize with dystrophin, myostatin inhibitor, stem cell based genetherapy, virus vectored gene therapy, non-sense codon skipping molecule,respiratory supportive care, cardiac supportive care, anti-inflammatoryagent, anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxidedonor, nitric oxide precursor, nitric oxide modulator, agent thatimprove mitochondrial function, and/or agent that promote calciumhomeostasis, wherein said SARM compound is represented by the structureof formula S-XXIII.

In another embodiment, the method of the invention for improving orpreserving lung function further increases the physical function of saidsubject. In one embodiment, the method of the invention for improving orpreserving lung function further increases the quality of life of saidsubject. In one embodiment, the method of the invention for improving orpreserving lung function further improves oxygen consumption of saidsubject. In one embodiment, the method of the invention for improving orpreserving lung function further improves mobility. In one embodiment,the method of the invention for improving or preserving lung functionfurther maintains energy expenditure of said subject. In one embodiment,the method of the invention for improving or preserving lung functionfurther reduces the methacholine (MeCh)-induced airway resistance. Inone embodiment, the method of the invention for improving or preservinglung function further reduces heart rates and breathing rates. In oneembodiment, the method of the invention for improving or preserving lungfunction further increases saturated oxygen levels. In one embodiment,the method of the invention for improving or preserving lung functionfurther treats or delays the onset of cardiac myopathy or cardiacfailure.

In one embodiment, the invention provides a method of improving cardiacfunction of a subject suffering from Duchenne muscular dystrophy,comprising the step of administering to said subject a selectiveandrogen receptor modulator (SARM) compound represented by the structureof formula S-XXIII:

or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide, orany combination thereof.

In one embodiment, the administering in the invention of improvingcardiac function comprises administering a pharmaceutical compositioncomprising the compound represented by the structure of formula S-XXIIIand/or its isomer, pharmaceutically acceptable salt, hydrate, N-oxide,or any combination thereof; and a pharmaceutically acceptable carrier.

In one embodiment, the invention provides a method of improving cardiacfunction of a subject suffering from Duchenne muscular dystrophy,comprising the step of administering to said subject a combinationcomprising a selective androgen receptor modulator (SARM) compound and atherapeutic agent selected from the group consisting of an exon-skippinganti-sense oligonucleotide, exon skipping anti-sense molecule, utrophinup-regulator, up-regulator of other proteins that co-localize withdystrophin, myostatin inhibitor, stem cell based gene therapy, virusvectored gene therapy, non-sense codon skipping molecule, respiratorysupportive care, cardiac supportive care, anti-inflammatory agent,anti-fibrotic agent, anti-oxidant, anabolic agent, nitric oxide donor,nitric oxide precursor, nitric oxide modulator, agent that improvemitochondrial function, and/or agent that promote calcium homeostasis,wherein said SARM compound is represented by the structure of formulaS-XXIII.

In one embodiment, the method of the invention for improving cardiacfunction further increases the physical function of said subject. In oneembodiment, the method of the invention for improving cardiac functionfurther increases the quality of life of said subject. In oneembodiment, the method of the invention for improving cardiac functionfurther improves oxygen consumption of said subject. In one embodiment,the method of the invention for improving cardiac function furtherimproves mobility. In one embodiment, the method of the invention forimproving cardiac function further maintains energy expenditure of saidsubject. In one embodiment, the method of the invention for improvingcardiac function further reduces the methacholine (MeCh)-induced airwayresistance. In one embodiment, the method of the invention for improvingcardiac function further treats or delays the onset of respiratoryinsufficiency or respiratory failure. In one embodiment, the method ofthe invention for improving cardiac function further reduces heart ratesand breathing rates. In one embodiment, the method of the invention forimproving cardiac function further increases saturated oxygen levels.

The muscular dystrophies are genetic diseases characterized byprogressive weakness and degeneration of the skeletal or voluntarymuscles that control movement. The muscles of the heart and some otherinvoluntary muscles are also affected in some forms of musculardystrophy. The nine major forms of muscular dystrophy (MD) are: Duchennemuscular dystrophy, myotonic dystrophy, Becker muscular dystrophy,limb-girdle muscular dystrophy, facioscapulhumeral muscular dystrophy,congenital muscular dystrophy, oculopharyngeal muscular dystrophy,distal muscular dystrophy and Emery-Dreifuss muscular dystrophy.

Muscular dystrophy can affect people of all ages. Although some formsfirst become apparent in infancy or childhood, others may not appearuntil middle age or later. Myotonic dystrophy is the most common ofthese diseases in adults. Myotonic dystrophy is an autosomal dominantgenetic disease that occurs in 1/8000 people and is characterized by 2types. Type I has a genetic defect in the DMPKgene whereas type II has agenetic defect in the CNBP gene. The mutation is an abnormal repeat of aDNA segment which presents a disease phenotype of variable severity inthe 2^(nd) or 3^(rd) decade of life. Symptoms of the disease may includeinter alia prolonged contractions (myotonia) of certain muscles,cataracts, cardiac conduction defects, balding, or male infertility.

Duchenne MD is the most common form, typically affecting children.Duchenne muscular dystrophy includes weakness and degeneration ofskeletal and voluntary muscle which is exacerbated by high impactexercise, muscle contractures that worsen mobility if not corrected, andscoliosis. Although braces and walkers provide some protection, declinesin physical function result in loss of ambulation during childhoodleading to wheelchair confinement, and eventually impaired cardiac(cardiomyopathy) or respiratory (diaphragm fibrosis) function leads todeath. Average life expectancy has improved (and rare cases of menliving into their 4^(th) or 5^(th) decade) as a result of betterrespiratory (glucocorticoids) and cardiac (ACE inhibitors, angiotensinreceptor blockers, and beta-blockers) supportive care but nodisease-modifying therapeutics exist.

Becker muscular dystrophy is a rarer and milder variation of Duchennemuscular dystrophy caused by DMD mutants that do not completely abrogatedystrophin glycoprotein complex function in males or more commonly isobserved in some female carriers (Duchenne muscular dystrophy is oftenasymptomatic in females).

In one embodiment, this invention provides therapeutic effects ondystrophic skeletal, cardiac, and diaphragm muscle, or may delay onsetor improve symptoms of loss of mobility/autonomy, cardiomyopathy, orrespiratory insufficiency in Duchenne muscular dystrophy or Beckermuscular dystrophy and other muscular dystrophy patients; byadministering the compound of this invention.

Muscle atrophy (MA) is characterized by wasting away or diminution ofmuscle and a decrease in muscle mass. For example, post-polio MA is amuscle wasting that occurs as part of the post-polio syndrome (PPS). Theatrophy includes weakness, muscle fatigue, and pain.

Another type of MA is X-linked spinal-bulbar muscular atrophy (SBMA—alsoknown as Kennedy's Disease). This disease arises from a defect in theandrogen receptor gene on the X chromosome, affects only males, and itsonset is in adulthood. Because the primary disease cause is an androgenreceptor mutation, androgen replacement is not a current therapeuticstrategy. There are some investigational studies where exogenoustestosterone propionate is being given to boost the levels of androgenwith hopes of overcoming androgen insensitivity and perhaps provide ananabolic effect. Still, use of supraphysiological levels of testosteronefor supplementation will have limitations and other potentially seriouscomplications.

Sarcopenia is a debilitating disease that afflicts the elderly andchronically ill patients and is characterized by loss of muscle mass andfunction. Further, increased lean body mass is associated with decreasedmorbidity and mortality for certain muscle-wasting disorders. Inaddition, other circumstances and conditions are linked to, and cancause muscle wasting disorders. For example, studies have shown that insevere cases of chronic lower back pain, there is paraspinal musclewasting.

Muscle wasting and other tissue wasting is also associated with advancedage. It is believed that general weakness in old age is due to musclewasting. As the body ages, an increasing proportion of skeletal muscleis replaced by fibrous tissue. The result is a significant reduction inmuscle power, performance and endurance.

Long term hospitalization due to illness or injury, or disusedeconditioning that occurs, for example, when a limb is immobilized, canalso lead to muscle wasting, or wasting of other tissue. Studies haveshown that in patients suffering injuries, chronic illnesses, burns,trauma or cancer, who are hospitalized for long periods of time, thereis a long-lasting unilateral muscle wasting, and a decrease in bodymass.

Injuries or damage to the central nervous system (CNS) are alsoassociated with muscle wasting and other wasting disorders. Injuries ordamage to the CNS can be, for example, caused by diseases, trauma orchemicals. Examples are central nerve injury or damage, peripheral nerveinjury or damage and spinal cord injury or damage. In one embodiment CNSdamage or injury comprise Alzheimer's diseases (AD), anger (mood);anorexia, anorexia nervosa, anorexia associated with aging and/orassertiveness (mood).

In another embodiment, muscle wasting or other tissue wasting may be aresult of alcoholism, and may be treated with the compounds andcompositions of the invention, representing embodiments thereof.

In one embodiment, the invention provides a use of SARM compounds asdescribed herein or its prodrug, analog, isomer, metabolite, derivative,pharmaceutically acceptable salt, pharmaceutical product, polymorph,crystal, impurity, N-oxide, hydrate or any combination thereof for thetreatment of a wasting disease, disorder or condition in a subject.

In one embodiment, the wasting disease, disorder or condition beingtreated is associated with chronic illness.

This invention is directed to treating, in some embodiments, any wastingdisorder, which may be reflected in muscle wasting, weight loss,malnutrition, starvation, or any wasting or loss of functioning due to aloss of tissue mass.

In some embodiments, wasting diseases or disorders, such as cachexia,malnutrition, tuberculosis, leprosy, diabetes, renal disease, chronicobstructive pulmonary disease (COPD), cancer, end stage renal failure,sarcopenia, emphysema, osteomalacia, or cardiomyopathy, may be treatedby the methods of this invention, via the administration of a SARMcompound as herein described, compositions comprising the same, with orwithout additional drugs, compounds, or agents, which provide atherapeutic effect for the condition being treated.

In some embodiments, wasting is due to infection with enterovirus,Epstein-Barr virus, herpes zoster, HIV, trypanosomes, influenza,coxsackie, rickettsia, trichinella, schistosoma or mycobacteria, andthis invention, in some embodiments, provides methods of treatmentthereof.

Cachexia is weakness and a loss of weight caused by a disease or as aside effect of illness. Cardiac cachexia, i.e. a muscle protein wastingof both the cardiac and skeletal muscle, is a characteristic ofcongestive heart failure. Cancer cachexia is a syndrome that occurs inpatients with solid tumors and hematological malignancies and ismanifested by weight loss with massive depletion of both adipose tissueand lean muscle mass.

Cachexia is also seen in acquired immunodeficiency syndrome (AIDS),human immunodeficiency virus (HIV)-associated myopathy and/or muscleweakness/wasting is a relatively common clinical manifestation of AIDS.Individuals with HIV-associated myopathy or muscle weakness or wastingtypically experience significant weight loss, generalized or proximalmuscle weakness, tenderness, and muscle atrophy.

In one embodiment, “Hypogonadism” is a condition resulting from orcharacterised by abnormally decreased functional activity of the gonads,with retardation of growth and sexual development.

In some embodiments, the present invention provides a method fortreating, reducing the incidence, delaying the onset or progression, orreducing and/or abrogating the symptoms associated with a wastingdisease in a subject. In one embodiment, the method comprisesadministering to a subject a composition comprising a compound of thisinvention and anti-cancer agent, an immunomodulating agent, anantidiabetic agent, an agent treating the cardiovascular system, anagent treating the gastrointestinal system, an agent treating thecentral nervous system, an agent treating a metabolic disease, an agenttreating a wasting disease, a gene therapy agent, an agent treating theendocrine system, vitamins, or a combination thereof. In someembodiments, wasting diseases comprise muscle injury, bed rest,immobility, nerve injury, neuropathy, diabetic neuropathy, alcoholicneuropathy, subacute combined degeneration of the spinal cord, diabetes,rheumatoid arthritis, motor neurone diseases, Duchenne musculardystrophy, carpal tunnel syndrome, chronic infection, tuberculosis,Addison's disease, adult SMA, limb muscle atrophy, alcoholic neuropathy,anorexia, anorexia nervosa, anorexia associated with cachexia, anorexiaassociated with aging, back tumour, dermatomyositis, hip cancer,inclusion body myositis, incontinentia pigmenti, intercostal neuralgia,juvenile rheumatoid arthritis, Legg-Calve-Perthes disease, muscleatrophy, multifocal motor neuropathy, nephrotic syndrome, osteogenesisimperfecta, post-polio syndrome, rib tumor, spinal muscular atrophy,reflex sympathetic dystrophy syndrome, or Tay-Sachs.

A wasting condition or disorder is defined herein as a condition ordisorder that is characterized, at least in part, by an abnormal,progressive loss of body, organ or tissue mass. A wasting condition canoccur as a result of a pathology such as, for example, cancer, or it canbe due to a physiologic or metabolic state, such as disusedeconditioning that can occur, for example, due to prolonged bed rest orwhen a limb is immobilized, such as in a cast, or with the occurrence ofmultiple wounds, including, for example, amputation, as occurs indiabetics, and other conditions, as will be appreciated by one skilledin the art. A wasting condition can also be age associated. The loss ofbody mass that occurs during a wasting condition can be characterized bya loss of total body weight, or a loss of organ weight such as a loss ofbone or muscle mass due to a decrease in tissue protein.

In one embodiment, the terms “muscle wasting” or “muscular wasting”,refer to the progressive loss of muscle mass and/or to the progressiveweakening and degeneration of muscles, including the skeletal orvoluntary muscles which control movement, cardiac muscles which controlthe heart, and smooth muscles. In one embodiment, the muscle wastingcondition or disorder is a chronic muscle wasting condition or disorder.“Chronic muscle wasting” is defined herein as the chronic (i.e.persisting over a long period of time) progressive loss of muscle massand/or to the chronic progressive weakening and degeneration of muscle.

The loss of muscle mass that occurs during muscle wasting can becharacterized by a muscle protein breakdown or degradation, by muscleprotein catabolism. Protein catabolism occurs because of an unusuallyhigh rate of protein degradation, an unusually low rate of proteinsynthesis, or a combination of both. Protein catabolism or depletion,whether caused by a high degree of protein degradation or a low degreeof protein synthesis, leads to a decrease in muscle mass and to musclewasting. The term “catabolism” has its commonly known meaning in theart, specifically an energy burning form of metabolism.

Muscle wasting can occur as a result of pathology, disease, condition ordisorders, including disorders for treatment via the methods of thisinvention, such as, for example, end stage renal failure.

In one embodiment, the wasting disease is cachexia or involuntary weightloss in a subject. In another embodiment, the present invention providesa method of treating, preventing, inhibiting, reducing or suppressingmuscle wasting in a subject suffering from a kidney disease. In oneembodiment, the present invention provides a method of treating,preventing, inhibiting, reducing or suppressing protein catabolism in asubject suffering from a kidney disease or disorder,

In some embodiments, the present invention provides a method fortreating, reducing the incidence, delaying the onset or progression, orreducing and/or abrogating the symptoms associated with a hypogonadalstate in a subject. In one embodiment, the present invention provides amethod for treating, reducing the incidence, delaying the onset orprogression, or reducing and/or abrogating the symptoms associated witha pharmacotherapy induced hypogonadal state in a subject. In someembodiments, hypogonadism is caused by treatments which alter thesecretion of hormones from the sex glands in both women and men. In someembodiments, hypogonadism may be “primary” or “central”. In primaryhypogonadism, the ovaries or testes themselves do not function properly.In some embodiments, hypogonadism may be induced by surgery, radiation,genetic and developmental disorders, liver and kidney disease,infection, or certain autoimmune disorders. In some embodiments,menopause is a form of hypogonadism.

In some embodiments, the present invention provides a method fortreating, reducing the incidence, delaying the onset or progression, orreducing and/or abrogating the symptoms associated with a combination ofdiseases and/or disorders in a subject as described hereinabove. In oneembodiment, the method comprises administering to a subject acomposition comprising a compound of this invention and an anti-canceragent, an immunomodulating agent, an antidiabetic agent, an agenttreating the cardiovascular system, an agent treating thegastrointestinal system, an agent treating the central nervous system,an agent treating a metabolic disease, an agent treating a wastingdisease, a gene therapy agent, an agent treating the endocrine system,an agent treating a dermatological disorder, an anti-infective agent, anagent treating the liver, an agent treating the kidney, vitamins, or acombination thereof.

It is to be understood that any method of this invention, as hereindescribed, encompasses the administration of a compound as hereindescribed, or a composition comprising the same, to the subject, inorder to treat the indicated disease, disorder or condition. The methodsas herein described each and/or all may further comprise administrationof an additional therapeutic agent as herein described, and as will beappreciated by one skilled in the art.

In some embodiments, the present invention provides a method forenhanced production such as milk, sperm, or egg. In some embodiments,the present invention provides a method for enhanced production of leanmeats or eggs. In some embodiments, the present invention provides amethod for increased productivity of feeds or stud livestock, forexample, increased sperm count, improved morphology of sperm, etc. Insome embodiments, the present invention provides a method for expandingthe productive life of farm animals, for example, egg-laying hens,milk-producing cows, etc, and/or enhanced herd health, for example,improved immune clearance, stronger animals.

In another embodiment, the compounds of this invention and compositionsas described herein are useful in promoting or speeding recoveryfollowing a surgical procedure.

In one embodiment, the present invention provides a use of a compound asdescribed herein for reducing a fat mass in a subject. In anotherembodiment the invention provides such methods for use of the compoundas described herein or its prodrug, analog, isomer, metabolite,derivative, pharmaceutically acceptable salt, pharmaceutical product,polymorph, crystal, impurity, N-oxide, hydrate or any combinationthereof, or a composition comprising the same.

In one embodiment, the present invention provides a use of a compound asdescribed herein for increasing a lean mass in a subject. In anotherembodiment such use comprises administration of a compound as describedherein or its prodrug, analog, isomer, metabolite, derivative,pharmaceutically acceptable salt, pharmaceutical product, polymorph,crystal, impurity, N-oxide, hydrate or any combination thereof.

In one embodiment the subject has a hormonal imbalance, disorder, ordisease. In another embodiment the subject has menopause.

Example 4 demonstrates that a compound of formula (S-II) is anabolic yetminimally androgenic, thus such compounds may be useful in treatingpatient groups in which androgens were contraindicated in the past.Compound of formula (S-II) was shown to stimulate muscle growth, whetherin the presence or absence of testosterone while exertinganti-proliferative effects on the prostate, thus, in one embodiment, themethods of this invention provide for restoring lost muscle mass inpatients with sarcopenia or cachexia.

In one embodiment, the compounds as herein described alter the levels ofleptin in a subject. In another embodiment, the compounds as hereindescribed decrease the levels of leptin. In another embodiment, thecompounds as herein described increase the levels of leptin in asubject. Leptin is known to have an effect on appetite or weight loss inobese mice, and thus has been implicated in obesity.

The compounds as herein described, in one embodiment, affectcirculating, or in another embodiment, tissue levels of leptin. In oneembodiment, the term ‘level/s of leptin’ refers to the serum level ofleptin. As contemplated herein, the compounds of the present inventionhave an effect on leptin in vitro and in vivo. Leptin levels can bemeasured by methods known to one skilled in the art, for example bycommercially available ELISA kits. In addition, leptin levels may bedetermined in in vitro assays, or in in vivo assays, by any method knownto a person skilled in the art.

Since leptin is implicated in controlling appetite, weight loss, foodintake, and energy expenditure, modulating and/or controlling the levelsof leptin is a useful therapeutic approach in treating, preventing,inhibiting or reducing the incidence of obesity in subjects sufferingfrom obesity. Modulating the level of leptin can result in a loss ofappetite, a reduction of food intake, and an increase in energyexpenditure in the subject, and thus may contribute to the control andtreatment of obesity.

The term “obesity” is defined, in one embodiment, as an increase in bodyweight beyond the limitation of skeletal and physical requirement, asthe result of excessive accumulation of fat in the body.

The term “obesity-associated metabolic disorder” refers, in oneembodiment, to a disorder which results from, is a consequence of, isexacerbated by or is secondary to obesity. Non-limiting examples of sucha disorder are osteoarthritis, type II diabetes mellitus, increasedblood pressure, stroke, and heart disease.

In addition, androgens have recently been shown to be involved incommitment of mesenchymal pluripotent cells into myogenic lineage and toblock differentiation into adipogenic lineage (Singh et al.,Endocrinology, 2003, Jul. 24). Accordingly, the compounds can be usefulin methods of blocking adipogenesis, and/or altering stem celldifferentiation, as described herein.

In another embodiment, this invention relates to a method of decreasing,suppressing, inhibiting or reducing appetite of a subject, comprisingthe step of administering to the subject a compound as herein describedand/or its analog, derivative, isomer, metabolite, pharmaceuticallyacceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug,polymorph, crystal, or any combination thereof, in an amount effectiveto decrease, suppress, inhibit or reduce the appetite of the subject.

In another embodiment, this invention relates to a method of alteringthe body composition of a subject, comprising the step of administeringto the subject a compound as herein described and/or its analog,derivative, isomer, metabolite, pharmaceutically acceptable salt,pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal,or any combination thereof, in an amount effective to alter the bodycomposition of the subject. In one embodiment, altering the bodycomposition comprises altering the lean body mass, the fat free bodymass of the subject, or a combination thereof.

In another embodiment, this invention relates to a method of alteringlean body mass or fat free body mass of a subject, comprising the stepof administering to the subject a compound as herein described and/orits analog, derivative, isomer, metabolite, pharmaceutically acceptablesalt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph,crystal, or any combination thereof, in an amount effective to alter thelean body mass or fat free body mass of the subject.

In another embodiment, this invention relates to a method of convertingfat to lean muscle in a subject, comprising the step of administering tothe subject a compound as herein described and/or its analog,derivative, isomer, metabolite, pharmaceutically acceptable salt,pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal,or any combination thereof, in an amount effective to convert fat tolean muscle in the subject. In another embodiment, this inventionrelates to a method of converting fat to lean muscle in a subjectsuffering from a wasting disorder. In another embodiment, this inventionrelates to a method of converting fat to lean muscle in a subjectsuffering from a wasting disorder wherein the wasting disorder is amuscular dystrophy. In another embodiment, this invention relates to amethod of converting fat to lean muscle in a subject suffering from awasting disorder wherein the wasting disorder is Duchenne musculardystrophy.

It is to be understood that any use of any of the compounds as hereindescribed may be used in the treatment of any disease, disorder orcondition as described herein, and represents an embodiment of thisinvention.

The following examples are presented in order to more fully illustratethe preferred embodiments of the invention. They should in no way,however, be construed as limiting the broad scope of the invention.

EXAMPLES Example 1 Synthesis of (S) Enantiomer of Compound of Formula II(S-II)

(2R)-1-Methacryloylpyrrolidin-2-carboxylic Acid

D-Proline, 14.93 g, 0.13 mol) was dissolved in 71 mL of 2 N NaOH andcooled in an ice bath; the resulting alkaline solution was diluted withacetone (71 mL). An acetone solution (71 mL) of methacryloyl chloride(13.56 g, 0.13 mol) and 2 N NaOH solution (71 mL) were simultaneouslyadded over 40 min to the aqueous solution of D-proline in an ice bath.The pH of the mixture was kept at 10-11° C. during the addition of themethacryloyl chloride. After stirring (3 h, room temperature), themixture was evaporated in vacuo at a temperature at 35-45° C. to removeacetone. The resulting solution was washed with ethyl ether and wasacidified to pH 2 with concentrated HCl. The acidic mixture wassaturated with NaCl and was extracted with EtOAc (100 mL×3). Thecombined extracts were dried over Na₂SO₄, filtered through Celite®, andevaporated in vacuo to give the crude product as a colorless oil.Recrystallization of the oil from ethyl ether and hexanes afforded 16.2g (68%) of the desired compound as colorless crystals: mp 102-103° C.(lit. mp 102.5-103.5° C.); the NMR spectrum of this compounddemonstrated the existence of two rotamers of the title compound. ¹H NMR(300 MHz, DMSO-d₆) δ 5.28 (s) and 5.15 (s) for the first rotamer, 5.15(s) and 5.03 (s) for the second rotamer (totally 2H for both rotamers,vinyl CH₂), 4.48-4.44 for the first rotamer, 4.24-4.20 (m) for thesecond rotamer (totally 1H for both rotamers, CH at the chiral canter),3.57-3.38 (m, 2H, CH₂), 2.27-2.12 (1H, CH), 1.97-1.72 (m, 6H, CH₂, CH,Me); ¹³C NMR (75 MHz, DMSO-d₆) δ for major rotamer 173.3, 169.1, 140.9,116.4, 58.3, 48.7, 28.9, 24.7, 19.5: for minor rotamer 174.0, 170.0,141.6, 115.2, 60.3, 45.9, 31.0, 22.3, 19.7; IR (KBr) 3437 (OH), 1737(C═O), 1647 (CO, COOH), 1584, 1508, 1459, 1369, 1348, 1178 cm⁻¹; [α]_(D)²⁶+80.8° (c=1, MeOH); Anal. Calcd. for C₉H₁₃NO₃: C, 59.00, H, 7.15, N,7.65. Found: C, 59.13, H, 7.19, N, 7.61.

(3R,8aR)-3-Bromomethyl-3-methyl-tetrahydro-pyrrolo[2,1-c][1,4]oxazine-1,4-dione

A solution of NBS (23.5 g, 0.132 mol) in 100 mL of DMF was addeddropwise to a stirred solution of the (methyl-acryloyl)-pyrrolidine(16.1 g, 88 mmol) in 70 mL of DMF under argon at room temperature, andthe resulting mixture was stirred 3 days. The solvent was removed invacuo, and a yellow solid was precipitated. The solid was suspended inwater, stirred overnight at room temperature, filtered, and dried togive 18.6 g (81%) (smaller weight when dried 34%) of the title compoundas a yellow solid: mp 152-154° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 4.69 (dd,J=9.6 Hz, J=6.7 Hz, 1H, CH at the chiral center), 4.02 (d, J=11.4 Hz,1H, CHH_(a)), 3.86 (d, J=11.4 Hz, 1H, CHH_(b)), 3.53-3.24 (m, 4H, CH₂),2.30-2.20 (m, 1H, CH), 2.04-1.72 (m, 3H, CH₂ and CH), 1.56 (s, 2H, Me);¹³C NMR (75 MHz, DMSO-d₆) δ 167.3, 163.1, 83.9, 57.2, 45.4, 37.8, 29.0,22.9, 21.6; IR (KBr) 3474, 1745 (C═O), 1687 (C═O), 1448, 1377, 1360,1308, 1227, 1159, 1062 cm⁻¹; [α]_(D) ²⁶+124.5° (c=1.3, chloroform);Anal. Calcd. for C₉H₁₂BrNO₃: C, 41.24, H, 4.61, N, 5.34. Found: C,41.46, H, 4.64, N, 5.32.

(2R)-3-Bromo-2-hydroxy-2-methylpropanoic Acid

A mixture of bromolactone (18.5 g, 71 mmol) in 300 mL of 24% HBr washeated at reflux for 1 h. The resulting solution was diluted with brine(200 mL), and was extracted with ethyl acetate (100 mL×4). The combinedextracts were washed with saturated NaHCO₃ (100 mL×4). The aqueoussolution was acidified with concentrated HCl to pH=1, which, in turn,was extracted with ethyl acetate (100 mL×4). The combined organicsolution was dried over Na₂SO₄, filtered through Celite®, and evaporatedin vacuo to dryness. Recrystallization from toluene afforded 10.2 g(86%) of the desired compound as colorless crystals: mp 107-109° C.; ¹HNMR (300 MHz, DMSO-d₆) δ 3.63 (d, J=10.1 Hz, 1H, CHH_(a)), 3.52 (d,J=10.1 Hz, 1H, CHH_(b)), 1.35 (s, 3H, Me); IR (KBr) 3434 (OH), 3300-2500(COOH), 1730 (C═O), 1449, 1421, 1380, 1292, 1193, 1085 cm⁻¹; [α]_(D)²⁶+10.5° (c=2.6, MeOH); Anal. Calcd. for C₄H₇BrO₃: C, 26.25, H, 3.86.Found: C, 26.28, H, 3.75.

Synthesis of(2R)-3-Bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide

Thionyl chloride (46.02 g, 0.39 mol) was added dropwise to a cooledsolution (less than 4° C.) of R-18 (51.13 g, 0.28 mol) in 300 mL of THFunder an argon atmosphere. The resulting mixture was stirred for 3 hunder the same condition. To this was added Et₃N (39.14 g, 0.39 mol) andstirred for 20 min under the same condition. After 20 min,5-amino-2-cyanobenzotrifluoride (40.0 g, 0.21 mol), 400 mL of THF wereadded and then the mixture was allowed to stir overnight at roomtemperature. The solvent was removed under reduced pressure to give asolid which was treated with 300 mL of H₂O, extracted with EtOAc (2×400mL). The combined organic extracts were washed with saturated NaHCO₃solution (2×300 mL) and brine (300 mL). The organic layer was dried overMgSO₄ and concentrated under reduced pressure to give a solid, which waspurified from column chromatography using CH₂Cl₂/EtOAc (80:20) to give asolid. This solid was recrystallized from CH₂Cl₂/hexane to give 55.8 g(73.9%) of(2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide(R-19) as a light-yellow solid.

¹H NMR (CDCl₃/TMS) δ 1.66 (s, 3H, CH₃), 3.11 (s, 1H, OH), 3.63 (d,J=10.8 Hz, 1H, CH₂), 4.05 (d, J=10.8 Hz, 1H, CH₂), 7.85 (d, J=8.4 Hz,1H, ArH), 7.99 (dd, J=2.1, 8.4 Hz, 1H, ArH), 8.12 (d, J=2.1 Hz, 1H,ArH), 9.04 (bs, 1H, NH). Calculated Mass: 349.99, [M-H]⁻ 349.0. M.p.:124-126° C.

Synthesis of(S)-3-(4-chloro-3-fluorophenoxy)-N-(4-cyano-3-(trifluoromethyl)phenyl)-2-hydroxy-2-methylpropanamide

A mixture of bromoamide((2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide,(R-19) 2.0 g, 5.70 mmol) and anhydrous K₂CO₃ (2.4 g, 17.1 mmol) washeated to reflux for 2 h and then concentrated under reduced pressure togive a solid. The resulting solid was treated with4-chloro-3-fluorophenol (1.3 g, 8.5 mmol) and anhydrous K₂CO₃ (1.6 g,11.4 mmol) in 50 mL of 2-propanol and was heated to reflux for 3 h, thenconcentrated under reduced pressure to give a solid. The residue wastreated with 100 mL of H₂O and then extracted with EtOAc (2×100 mL). Thecombined EtOAc extracts were washed with 10% NaOH (4×100 mL) and brine,successively. The organic layer was dried over MgSO₄ and thenconcentrated under reduced pressure to give an oil which was purified bycolumn chromatography using EtOAc/hexane (50:50) to give a solid whichwas recrystallized from CH₂Cl₂/hexane to give 1.7 g (70.5%) of(S)-3-(4-chloro-3-fluorophenoxy)-N-(4-cyano-3-(trifluoromethyl)phenyl)-2-hydroxy-2-methylpropanamideas a colorless solid.

¹H NMR (CDCl₃/TMS) δ 1.60 (s, 3H, CH₃), 3.28 (s, 1H, OH), 3.98 (d,J=9.05 Hz, 1H, CH), 6.64-6.76 (m, 2H, ArH), 7.30 (d, J=8.67 Hz, 1H,ArH), 7.81 (d, J=8.52 Hz, 1H, ArH), 7.96 (q, J=2.07, 8.52 Hz, 1H, ArH),8.10 (d, J=2.07 Hz, 1H, ArH), 9.10 (s, 1H, NH). Calculated Mass: [M-H]⁻414.9. Mp: 132-134° C.

Example 2 Metabolic Stability of the Compounds of this Invention

Metabolic stability assays were performed in order to assess the invitro half-life of the S-isomer of the compound of formula II (S-II)when incubated with human liver microsomes. The data generated wastransformed to determine intrinsic clearance values. In a separateexperiment, permeability across human, intestinal epithelial monolayers(Caco-2 cells) was used as a measure of intestinal permeability as wellas an indicator of efflux potential. Caco-2 cells are often used as anearly screening surrogate for oral bioavailability. Microsomal half-lifecan be converted to in vitro clearance values as a means to predicthepatic intrinsic clearance. Intrinsic clearance is defined as thefunctional ability of the liver to metabolize a drug or other compound.

Materials and Methods:

Metabolic Stability Measured in Human Liver Microsomes:

Compound of formula S-II in this study was incubated at a finalconcentration of 0.6 μM. Microsome reactions were performed under eitherPhase I or “Phase I and II” conditions, where indicated. Compound stocks(10 mM ACN) were initially diluted to a concentration of 60 μM (in 60%ACN/H₂O) resulting in a “working stock” solution of 100×. Human livermicrosomes were utilized at a final concentration of 0.6 mg/ml.Duplicate wells were used for each time point (0, 6, 10, 30, and 60minutes). Reactions were carried out at 37° C. in a shaking water bath,and the final concentration of solvent was kept constant at 0.6%. Thefinal volume for each reaction was 600 μl, comprised of 368 μl of 100 mMKPO₄ buffer, (pH 7.4); 12.6 μl of HLM (from a 20 mg/ml stock); 6 μl of100× “working stock” drug compound, and 126 μl of NRS “master mix”solution. At each time point, 100 μl of reaction was removed and addedto a sample well containing 100 μl of ice-cold, 100% ACN (plus internalstandards), to stop the reaction. The NRS “master mix” is a solution ofglucose-6-phosphate dehydrogenase, NADP⁺, MgCl₂, andglucose-6-phosphate, prepared per manufacturer's instructions (BDBiosciences, Waltham, Mass.). Each 6.0 ml stock of NRS “master mix”solution contains 3.8 ml H₂O, 1.0 ml solution “A” (Cat. #461220), and0.2 ml solution “B” (Cat. #461200). Human liver microsomes (lot#0610279, Xenotech Corp.) represented a pool of 60 donors.

Samples were centrifuged at 3,000 rpm for 10 minutes at 4° C. to removedebris and precipitate protein. Approximately 160 μl of supernatant wassubsequently transferred to a new sample block for analysis. Theconcentration of parent drug remaining in each well (expressed aspercent remaining versus Time ‘0’, at the beginning of the reaction) wasmeasured by LC/MS, as detailed below. The intrinsic clearance rates(CL_(int)) were calculated from 0-60 minutes based on first order decaykinetics as a function of microsomal protein concentration.

Permeability Across Human, Intestinal Epithelial Monolayers:

Permeability was measured in the Apical (pH 6.6) to Basolateral (pH 7.4)and Basolateral (pH 7.4) to Apical (pH 6.6) directions across polarized,Caco-2 epithelial monolayers. Compound stocks (10 mM acetonitrile) weretested in the study at a final concentration of 10 μM. The concentrationof drug in the receiver well was measured by LC/MS/MS using a standardcurve. The apparent permeability (P_(app)) for each compound wascalculated, and values (A−B) were classified as: Poor (P_(app): <1), Low(P_(app) 1-2), Medium (P_(app) 2-10) or High (P_(app)>10).P _(app)(×10⁻⁶ cm/sec)=Amount transported/(Area*Initialconcentration*Time)P _(app)(cm/s)=[V/(A*Ci)]*(Cf/T)V=volume of the receptor chamber (ml, or cm³)A=area of the membrane insert (cm²)Ci=initial concentration of drug (μM)Cf=final concentration of drug (μM)T=assay time (seconds)Analytical Methods:

All samples were analyzed on the MDS/Sciex API4000 Q Trap system withelectrospray ionization (ESI) in the positive or negative SIM mode,depending on the compounds. The mobile phases were isocratic at 30% A(0.1% formic acid in water) and 70% B (0.1% formic acid in acetonitrile)with a flow rate of 0.4 mL/min. A Phenomenex Luna Phenyl-Hexyl column(60×2.0 mm ID, 6μ) was used. The injection volume was 10 μL. The totalrun time per sample was 1.6 to 3.0 minutes. Tamoxifen and diclofenacwere used as internal standards for the positive and negative mode,respectively. The percentage of parent drug compound remaining aftereach time point was determined relative to the initial measuredconcentration at the beginning of the reaction (To min).

Data Analysis:

For half-life determination, data was fitted using GraphPad Prism, v4.03 with the non-linear regression equation “one phase exponentialdecay” defined as: Y=Span*exp(−K*X)+Plateau (decays to Plateau with afirst-order rate constant, K). “−K” is the slope of the curve. The halflife (minutes), T_(1/2), =ln 2/−K and is therefore defined as −0.693/K,a.k.a. −0.693/slope). Intrinsic Clearance (μl/min/mg protein) is definedas: CL_(int)=0.693*(1/T_(1/2))*(ml incubation/mg protein)*1000; Thisequation can also be expressed as (K*1000)/microsome concentration.

Results:

TABLE 1 Metabolic Stability Measured in Human Liver Microsomes: CL_(int)CL_(int) Compound Half Life (μl/min/mg) Half Life (μl/min/mg) having(minutes) Phase I (minutes) Phase I + formula Phase I only only PhaseI + II II S-II Stable <1 Stable <1

The results had shown that in vitro half-life as determined from themicrosomal assays demonstrated that compound of formula S-II under bothphase I and phase I/II metabolic conditions. As shown in Table 1, thecompound did not exhibit an intrinsic clearance (CL_(int)) value greaterthan 10 μl/min/mg. It is generally accepted that an in vitro CL_(int)value of less than 10 μl/min/mg protein represents favorable metabolicstability of the test compound. Compound of formula S-II exhibited lowclearance in human liver microsomes. In conclusion, based on the datareported herein, compound of formula S-II exhibited favorable metabolicstability profiles in vivo studies.

Example 3 Androgen Receptor Binding Affinity of SARMs

Materials and Methods:

The androgen receptor (AR) binding affinity of SARMs was determined byusing an in vitro competitive radioligand binding assay with[17α-methyl-³H]-mibolerone ([³H]MIB, PerkinElmer), a high affinity ARligand. Recombinant androgen receptor ligand binding domain (AR LBD) wascombined with [³H]MIB in buffer A (10 mM Tris, pH 7.4, 1.6 mM disodiumEDTA, 0.26 M sucrose, 10 mM sodium molybdate, 1 mM PMSF) to determinethe equilibrium dissociation constant (K_(d)) of [³H]MIB. Protein wasincubated with increasing concentrations of [³H]MIB with and without ahigh concentration of unlabeled MIB in order to determine total andnon-specific binding. Non-specific binding was then subtracted fromtotal binding to determine specific binding and graphed using SigmaPlotand non-linear regression for ligand binding curve with one sitesaturation to determine the K_(d) of MIB (1.84 nM). In addition, theconcentration of [³H]MIB required to saturate AR LBD was determined tobe 4 nM.

Compound of formula S-II was tested in a range of concentrations from10⁻¹¹ to 10⁻⁶ M using the conditions described above. Followingincubation, plates were harvested with GF/B filters on the Unifilter-96Harvester (PerkinElmer) and washed three times with ice-cold buffer B(60 mM Tris, pH 7.2). The filter plates were dried at RT, then 36 μlMicroscint-O cocktail was added to each well and sealed with TopSeal-A.The receptor bound radioligand was then determined with the TopCount®NXT Microplate Scintillation Counter (PerkinElmer).

The specific binding of [³H]MIB at each concentration of SARM wasdetermined by subtracting the nonspecific binding of [³H]MIB (determinedby incubating with 10⁻⁶ M unlabeled MIB), and expressed as a percentageof the specific binding in the absence of each SARM. The concentrationof SARM required to decrease the [³H]MIB binding by 60%, IC₆₀ value, wasdetermined by computer-fitting the data with SigmaPlot and non-linearregression with the standard curve four parameter logistic curve. Theequilibrium binding constant (K) of each compound was then determinedwith the following equation:K _(i) =K _(d) ×IC ₆₀/(K _(d) +L)where K_(d) is the equilibrium dissociation constant of [³H]MIB (1.84nM), and L is the concentration of [³H]MIB (4 nM).Results:

The binding affinity for compound of formula S-II was tested in theradioligand binding assay with AR LBD as the receptor with K_(i)(nM)=8.1.

Example 4 Preclinical Anabolic and Androgenic Pharmacology of Compoundof Formula S-II in Intact and Castrate Male Rats

Anabolic and androgenic efficacy of compound of formula S-IIadministered by daily oral gavage was tested. The S-isomer of compoundof formula II was synthesized and tested as described herein.

Materials and Methods:

Male Sprague-Dawley rats weighing approximately 200 g were purchasedfrom Harlan Bioproducts for Science (Indianapolis, Ind.). The animalswere maintained on a 12-h light/dark cycle with food (7012C LM-485Mouse/Rat Sterilizable Diet, Harlan Teklad, Madison, Wis.) and wateravailable ad libitum. The animal protocol was reviewed and approved. Theanabolic and androgenic activity of the compound of formula S-II wasstudied in intact animals, acutely orchidectomized (ORX) animals andchronically (9 days) ORX rats.

The test article for this study was weighed and dissolved in 10% DMSO(Fisher) diluted with PEG 300 (Acros Organics, N.J.) for preparation ofthe appropriate dosage concentrations. The animals were housed in groupsof 2 to 3 animals per cage. Animals were randomly assigned to one ofseven groups consisting of 4 to 5 animals per group. Control groups(intact and ORX) were administered vehicle daily. Compound of formulaS-II was administered via oral gavage at doses of 0.01, 0.03, 0.1, 0.3,0.75, and 1 mg/day to both intact and ORX groups. Where appropriate,animals were castrated on day one of the study. Treatment with compoundof formula S-II began nine days post ORX and was administered daily viaoral gavage for fourteen days.

The animals were sacrificed under anesthesia (ketamine/xyalzine, 87:13mg/kg) and body weights were recorded. In addition, ventral prostate,seminal vesicles, and levator ani muscle were removed, individuallyweighed, normalized to body weight, and expressed as a percentage ofintact control. Student's T-test was used to compare individual dosegroups to the intact control group. Significance was defined a priori asa P-value <0.05. Ventral prostate and seminal vesicle weights wereevaluated as a measure of androgenic activity, whereas levator animuscle weight was evaluated as a measure of anabolic activity. Blood wascollected from the abdominal aorta, centrifuged, and sera were frozen at−80° C. prior to determination of serum hormone levels. Serumluteinizing hormone (LH) and follicle stimulating hormone (FSH)concentrations were determined.

Results:

A series of dose-response studies in intact and castrated rats in orderto evaluate the potency and efficacy of compound of formula S-II in bothandrogenic (prostate and seminal vesicles) and anabolic (levator animuscle) tissue was conducted. In intact animals, compound of formulaS-II treatment resulted in decreases in the weight of both prostate andseminal vesicles while the levator ani muscle weight was significantlyincreased. Levator ani muscle weight following compound of formula S-IItreatment were 100%±10%, 98%±7%, 110%±5%, 110%±5%, 125%±10%, and129%±10% of intact controls following doses of 0.01, 0.03, 0.1, 0.3,0.75, and 1 mg/day, respectively. The prostate weights were 117%±20%,98%±15%, 82%±20%, 62%±5%, 107%±30%, and 110%±14% of intact controlsfollowing doses of 0.01, 0.03, 0.1, 0.3, 0.75, and 1 mg/day,respectively. These results are significant since current androgentherapies are contraindicated in some patient populations due to theproliferative androgenic effects in prostate and breast tissues.However, many patients in these populations could benefit from theanabolic actions of androgens in muscle and bone. Since compound offormula (S-II) exhibited tissue selective anabolic effects, it may bepossible to treat patient groups in which androgens were contraindicatedin the past.

In castrated, ORX animals, prostate weights following compound offormula S-II treatment were 10%±3%, 12%±3%, 26%±7%, 39%±6%, 60%±14%,88%±16%, and 123%±22% of intact controls following doses of 0, 0.01,0.03, 0.1, 0.3, 0.75, and 1 mg/day, respectively (FIG. 2). Similarly,seminal vesicle weights were 11%±1%, 11%±1%, 11%±1%, 27%±14%, 58%±18%,86%±12%, and 100%±8% of intact controls following doses of 0, 0.01,0.03, 0.1, 0.3, 0.75, and 1 mg/day, respectively (FIG. 2). Significantincreases were seen in levator ani muscle weights in all dose groups,when compared to intact controls. The levator ani muscle weights were48%±8%, 50%±5%, 62%±6%, 89%±10%, 118%±6%, 134%±8% and 129%±14% of intactcontrols corresponding to 0, 0.01, 0.03, 0.1, 0.3, 0.75, and 1.0 mg/daydose groups, respectively (FIG. 2).

Testosterone propionate (TP) andS-3-(4-acetylaminophenoxy)-2-hydroxy-2-methyl-N-(4-nitro-3-trifluoromethylphenyl)propionamide(S-4), maximally stimulated the levator ani muscle weight to 104% and101%, respectively. These data show that compound of formula S-IIexhibited significantly greater efficacy and potency than either TP orS-4. As a whole, these data show that compound of formula S-II is ableto stimulate muscle growth in the presence or absence of testosteronewhile exerting anti-proliferative effects on the prostate. These datashow that that compound of formula S-II restores lost muscle mass inpatients with sarcopenia or cachexia. Additionally, theantiproliferative effects of compound of formula S-II on the prostatemay allow some patient populations, in which androgens are currentlycontraindicated, access to anabolic agents.

Compound of formula S-III exhibited anabolic muscle/prostate ratio incastrated rats of 4.10, 2.39, 2.28, 1.97, 1.53, and 1.05 following dosesof 0.01, 0.03, 0.1, 0.3, 0.75 and 1 mg/day, respectively.

Pharmacology results following 1 mg/day of compound of formula S-IIIexhibited that prostate weight was 110%±14% of intact control andlevator ani muscle weight was 129%±10% of intact control. Compound offormula S-II maintained prostate weight following orchidectomy at123±22% of intact controls and levator ani muscle weight at 129±14% ofintact controls. A range of between 0.1 mg/day to 0.3 mg/day of compoundof formula S-II restored 100% of levator ani muscle weight, whilebetween 39 to 60% prostate weight was restored.

Example 5 In Vitro CYP Inhibition Assay

Materials and Methods:

P450 enzyme inhibition was measured using human cDNA-expressed CYP3A4,2D6, 2C19, 2C9, and 1A2 recombinant enzymes and fluorogenic substrates(coumarin analogues) that are converted to fluorescent products. Theanalogues utilized for each isoenzyme are as follows:7-benzyloxy-trifluoromethylcoumarin, (BFC) for 3A4;3-[2-(N,N-diethyl-N-methyl amino)ethyl]-7-methoxy-4-methylcoumarin,(AMMC) for 2D6; 3-cyano-7-ethoxycoumarin, (CEC) for 2C19 and 1A2; and7-methoxy-4-trifluoromethylcoumarin, (MFC) for 2C9. These substrateswere utilized at a single concentration (either 50 μM or 75 μM) at ornear the apparent K_(m) for each substrate. Fluorescence intensity wasmeasured using a Wallac 1420 Victor³ Multi-label Counter Model(Perkin-Elmer, Wellesley, Mass.), with an excitation wavelength filterof 405 nm, and an emission filter of 460 nm (535 nm for the 3A4 and 2C9substrates). Compound stocks (10 mM in a 4:1 ratio of acetonitrile:DMSO)were tested in this study using an 8-point dose response curve induplicate (ranging from 0.15 μM-20.0 μM). The concentration ofacetonitrile was kept constant at 0.4%, and the reaction was carried outat 37° C. for 30 minutes. Averages (minus background) and IC₅₀ valueswere calculated.

Results:

The in vitro screening results for potential drug-drug interactions(DDI) of SARM compound of formula S-II is presented in Table 2:

TABLE 2 CYP (P450) Inhibition, IC₅₀ (μM) Compound 3A4 2D6 2C19 2C9 1A2S-II >20 17.7 2.4 1.3 >20

Example 6 Pharmacokinetics of Compound of Formula S-II in Dogs

In order to determine the pharmacokinetics of compound of formula S-II,the compound was administered to beagle dogs perorally, and circulatingplasma levels, terminal elimination half-life (t_(1/2)), total bodyclearance (CL), terminal volume distribution (Vz) and absolutebioavailability (F %) (Table 3) were determined.

TABLE 3 Compound S-II T_(1/2) (hr)   37 ± 26.8 CL (mL/min/kg) 0.36 ±0.12 Vz (mL/kg) 1266 ± 352  F % 72.5%

Example 7 Anabolic and Androgenic Activity of SARM Compounds in Intactand Castrated Male Rats

The in vivo pharmacological activity of each synthetic AR ligand (listedin Table 4, below) was examined in five male Sprague-Dawley ratsweighing approximately 200 g. Animals were castrated via a scrotalincision under anesthesia 24 h before drug treatments and received dailysubcutaneous injections of the compound of interest at a dose rate of 1mg/d for 14 d. All compounds of interest were freshly dissolved invehicle containing dimethylsulfoxide (5%, vol/vol) in polyethyleneglycol 300 before dose administration. An additional two groups ofanimals with or without castration received vehicle only and served ascastrated or intact control groups, respectively. Animals were killed atthe end of the treatment. Plasma samples were collected and stored at−80° C. for future use. The ventral prostate, seminal vesicles, andlevator ani muscle were removed, cleared of extraneous tissue, andweighed. All organ weights were normalized to body weight and compared.The weights of prostate and seminal vesicles were used to evaluateandrogenic activity, whereas the levator ani muscle weight was used as ameasure of anabolic activity. Ventral prostate weights in SARM treatedcastrated rats were all (except C-6) statistically lowered than intactcontrol (FIG. 4). Whereas levator ani weights in castrated rats treatedwith SARM: C-3, C-6, C-8, C-10, C-11, or C-18 demonstrated support ofmuscle weight same as or in excess of intact control (FIG. 3). FurtherS-1, C-1, C-4, C-22, and C-23 demonstrated levator ani agonism of >75%of intact control (FIG. 3) vs. <25% of intact control in all of thesecases for ventral prostate (FIG. 4). This demonstrated tissue-selectiveanabolism for a variety of SARMs of this invention. The results aregraphically depicted in FIGS. 3 and 4.

TABLE 4

Compound R₁ R₂ R₃ R₄ R₅ S-1  H H F H H C-1  F H F H H C-2  CH₃ H F H HC-3  H F F H H C-4  H Cl F H H C-6  H F Cl H H C-8  F H Cl H H C-10 H ClCl H H C-11 H F NO₂ H H C-12 F H NO₂ H H C-13 F F F H H C-14 F F H F HC-17 F H F H F C-18 F H F F H C-22 Cl H Cl Cl H C-23 F F F F F

Example 8 Effects of SARM Compounds on Growth Performance and CarcassComposition of Finishing Pigs

Materials and Methods:

The effects of SARM as represented by compound of formula S-II on growthperformance and carcass composition of finishing pigs was examined.Forty crossbred barrows, (TR4×C22) with an initial weight of 209.4 lbwere used for this 28-d experiment. Pigs were blocked by weight andallotted to one of four treatments with ten replicate pens pertreatment. Pigs were housed with one pig per pen in an environmentallycontrolled finishing barn with 4 ft×4 ft slatted pens.

All animals were fed a corn-soybean meal diet with 1% corn oil. For thetreated groups, appropriate quantities of a compound of formula S-II(referred to as SARM in the text and figures/tables of this example)were dissolved in 100 mL of polysorbate (Tween®) 80 and diluted with 20lbs of corn oil prior to incorporation into the test diet. Final SARMconcentrations were 1, 3, and 10 ppm. All animals were fed theirrespective diets for the duration of the study. The test diets contained1.07% TID lysine. Prior to being placed on study, all pigs were fed acommon corn-soybean meal diet formulated to 0.75% TID lysine.

Pigs were allowed ad libitum access to feed and water. Pigs and feederswere weighed on day 7, 14, 21, and 28 to calculate average daily gain(ADG), average daily feed intake (ADFI), and feed-to-gain ratio (F/G orF:G). Each pen served as an experimental unit for all statisticalanalysis.

Pigs were slaughtered at the Kansas State University Meats Laboratory atthe end of the study for collection of individual carcass data. At 24hours postmortem, the right side of the carcass was frozen at −40° C.for approximately 1 h. After freezing, sides were ground once through agrinder equipped with a 19 mm die, then mixed and ground through asecond grinder equipped with a 9.5 mm die. A sub sample of groundcarcass was then chemically analyzed to determine percentages of crudeprotein, moisture/dry matter, lipid, and ash. Carcass measurements weredone on the left side of the carcass, and a sample of lean and fat wastaken from the longissimus at approximately the 10^(th) rib.

The data were analyzed as a randomized complete-block design. Analysisof variance was performed by using the MIXED procedure of SAS. Linearand quadratic contrasts were used to evaluate the effects of increasingthe level of the SARM on growth and carcass performance.

Results:

Although there were few statistical differences observed in the measuredparameters due to the small group sizes and individual housing ofexperimental animals, we observed positive trends in several keyparameters as shown in FIG. 5. Raw data are also summarized below. SARMincreased average daily gain (ADG) over the course of the study (FIG.5A), decreased feed to gain ratio (F:G) (FIG. 5B), increased fat freelean gain per day (FIG. 5C), and dramatically increased ADG for days21-28 (FIG. 5D).

Further, SARM treatment resulted in significantly increased Day 0-7 F:Gand decreased Day 8-14 average daily feed intake (ADFI). Table 5 showsthe weekly as well as the overall ADG, ADFI, and F:G data from thestudy.

TABLE 5 Vehicle SARM, ppm Probability, P< Parameter Control 1 3 10Linear Quadratic Day 0-7 ADG 2.51 2.89 2.37 2.17 0.07 0.87 ADFI 7.667.56 7.54 7.4 0.37 0.8 F:G 3.24 2.67 3.27 3.85 0.05 0.62 Day 8-14 ADG2.74 2.59 2.65 2.37 0.14 0.94 ADFI 8.55 8.74 8.5 7.89 0.04 0.67 F:G 3.543.52 3.3 3.46 0.83 0.57 Day 15-21 ADG 2.25 2.33 2.65 2.43 0.39 0.21 ADFI7.99 8.33 8.61 7.78 0.45 0.09 F:G 3.7 3.88 3.4 3.33 0.19 0.71 Day 22-28ADG 2.77 3.15 3.15 3.03 0.92 0.19 ADFI 8.19 8.51 8.89 8.33 0.79 0.26 F:G3.12 2.72 2.87 2.83 0.95 0.24 Day 0-28 ADG 2.57 2.74 2.7 2.5 0.28 0.31ADFI 8.1 8.28 8.38 7.85 0.29 0.27 F:G 3.2 3.04 3.11 3.17 0.56 0.45

At the time of sacrifice, a carcass composition analysis was performed.The data from this analysis are presented in Table 6. Trends towardsincreased lean mass and decreased fat were observed. SARM-treated pigsshowed a 7 to 10% decrease in first rib fat, 3 to 8% decrease in lastrib fat, 2 to 11% decrease in last lumbar fat, 6 to 14% decrease in10^(th) rib fat, and up to a 4% increase in loin eye area (LEA). Treatedanimals also demonstrated up to a 2% improvement in lean percent andpounds of fat free lean. However, due to the variability in this study,none of these measurements reached significance.

TABLE 6 Vehicle SARM, ppm Probability, P< Parameter Control 1 3 10Linear Quadratic Heart Wt, lb 0.99 1.04 1.07 1.01 0.92 0.08 Liver Wt, lb4.19 4.58 4.95 4.84 0.05 0.02 Kidney Wt, lb 1.08 1.2 1.18 1.16 0.38 0.3Dress, % 70.3 69.6 69.3 70 0.52 0.23 1^(st) rib fat, in 1.59 1.47 1.481.43 0.21 0.31 Last rib fat, in 0.94 0.9 0.91 0.86 0.57 0.79 in 0.840.77 0.82 0.74 0.16 0.75 10^(th) rib fat, in 0.86 0.78 0.8 0.74 0.35 0.7LEA, in² 7.27 7.26 7.22 7.59 0.09 0.41 Lean, % 52.4 53.2 52.9 54.3 0.161 Fat free lean, lb 104.2 106.9 105.5 106.7 0.46 0.79 ^(a) A total of 40barrows were used (carcass weight of 199 lb)

Table 7 shows the complete data set for all parameters which werecollected in this Example.

TABLE 7 SARM SARM SARM Control (1 PPM) (3 PPM) (10 PPM) ADG (Days 0-7)2.51 2.89 2.37 2.17 ADG (Days 8-14) 2.74 2.59 2.65 2.37 ADG (Days 15-21)2.25 2.33 2.65 2.43 ADG (Days 22-28) 2.77 3.15 3.15 3.03 ADG Total 2.572.74 2.70 2.50 ADFI (Days 0-7) 7.66 7.56 7.54 7.40 ADFI (Days 8-14) 8.558.74 8.50 7.89 ADFI (Days 15-21) 7.99 8.33 8.61 7.78 ADFI (Days 22-28)8.19 8.51 8.89 8.33 F/G (Days 0-7) 3.24 2.67 3.27 3.85 F/G (Days 8-14)3.54 3.52 3.30 3.46 F/G (Days 15-21) 3.70 3.88 3.40 3.33 F/G (Days22-28) 3.12 2.72 2.87 2.83 F/G Total 3.20 3.04 3.11 3.17 Gain (Days 0-7)17.5 20.3 16.6 15.2 Gain (Days 8-14) 19.2 18.2 18.6 16.6 Gain (Days15-21) 15.7 16.3 18.5 17.0 Gain (Days 22-28) 19.4 22.0 22.0 21.2 InitialWt 209 209 209 209 Live Wt 284 289 288 281 L side 97.6 98.0 97.7 96.8 Rside 101 103 102 99.8 Carcass Wt 199 201 200 197 Dress % 70% 70% 69% 70%1st rib fat 1.59 1.47 1.48 1.43 Last rib fat 0.935 0.900 0.905 0.855Last lumbar fat 0.835 0.770 0.815 0.740 10th rib fat 0.855 0.780 0.8000.735 LEA 7.27 7.26 7.22 7.59 lb FFL 104 107 106 107 lbs FFL/D 0.7270.822 0.774 0.814 Heart Wt 0.988 1.04 1.07 1.01 Liver Wt 4.19 4.58 4.954.84 Kidney Wt 1.08 1.20 1.18 1.16

The potential for using SARM (compound of formula S-II) to improvefinishing characteristics in food-animals was demonstrated. Feeding theSARM greatly improved ADG and F:G while reducing carcass crude fat. Someof the greatest improvements in these parameters were noted as thelowest dose (1 ppm). Lesser effects at high dose have been observed withother SARMs. Taken as a whole, these data support that SARM treatmentwould improve carcass composition and growth performance which are keyfactors in the economics of swine production.

Example 9 Effects of SARM Compounds on Growth Performance and CarcassComposition of Finishing Pigs Compared with Paylean®

Further studies with lower doses, larger group sizes and a directcomparison to ractopamine are conducted. As a direct competitor toractopamine, the SARM treated animals demonstrate the highest ADGs inthe fourth week of treatment. By the fourth week of treatment withractopamine the animals are desensitized to the beta-agonist and theproducers are seeing diminished returns in lean mass gain. Therefore,longer treatment periods (>28 days) may be advantageous to the producerswhen feeding a SARM than when feeding ractopamine.

Example 10 Synthesis of Compound of Formula S-XXIII (FIG. 6)

(2R)-1-Methacryloylpyrrolidin-2-carboxylic Acid

D-Proline, 14.93 g, 0.13 mol) was dissolved in 71 mL of 2 N NaOH andcooled in an ice bath; the resulting alkaline solution was diluted withacetone (71 mL). An acetone solution (71 mL) of methacryloyl chloride(13.56 g, 0.13 mol) and 2 N NaOH solution (71 mL) were simultaneouslyadded over 40 min to the aqueous solution of D-proline in an ice bath.The pH of the mixture was kept at 10-11° C. during the addition of themethacryloyl chloride. After stirring (3 h, room temperature), themixture was evaporated in vacuo at a temperature at 35-45° C. to removeacetone. The resulting solution was washed with ethyl ether and wasacidified to pH 2 with concentrated HCl. The acidic mixture wassaturated with NaCl and was extracted with EtOAc (100 mL×3). Thecombined extracts were dried over Na₂SO₄, filtered through Celite®, andevaporated in vacuo to give the crude product as a colorless oil.Recrystallization of the oil from ethyl ether and hexanes afforded 16.2g (68%) of the desired compound as colorless crystals: mp 102-103° C.(lit. mp 102.5-103.5° C.); the NMR spectrum of this compounddemonstrated the existence of two rotamers of the title compound. ¹H NMR(300 MHz, DMSO-d₆) δ 5.28 (s) and 5.15 (s) for the first rotamer, 5.15(s) and 5.03 (s) for the second rotamer (totally 2H for both rotamers,vinyl CH₂), 4.48-4.44 for the first rotamer, 4.24-4.20 (m) for thesecond rotamer (totally 1H for both rotamers, CH at the chiral center),3.57-3.38 (m, 2H, CH₂), 2.27-2.12 (1H, CH), 1.97-1.72 (m, 6H, CH₂, CH,Me); ¹³C NMR (75 MHz, DMSO-d₆) δ for major rotamer 173.3, 169.1, 140.9,116.4, 58.3, 48.7, 28.9, 24.7, 19.5: for minor rotamer 174.0, 170.0,141.6, 115.2, 60.3, 45.9, 31.0, 22.3, 19.7; IR (KBr) 3437 (OH), 1737(C═O), 1647 (CO, COOH), 1584, 1508, 1459, 1369, 1348, 1178 cm⁻¹; [α]_(D)²⁶+80.8° (c=1, MeOH); Anal. Calcd. for C₉H₁₃NO₃: C, 59.00, H, 7.15, N,7.65. Found: C, 59.13, H, 7.19, N, 7.61.

(3R,8aR)-3-Bromomethyl-3-methyl-tetrahydro-pyrrolo[2,1-c][1,4]oxazine-1,4-dione

A solution of NBS (23.5 g, 0.132 mol) in 100 mL of DMF was addeddropwise to a stirred solution of the (methyl-acryloyl)-pyrrolidine(16.1 g, 88 mmol) in 70 mL of DMF under argon at room temperature, andthe resulting mixture was stirred 3 days. The solvent was removed invacuo, and a yellow solid was precipitated. The solid was suspended inwater, stirred overnight at room temperature, filtered, and dried togive 18.6 g (81%) (smaller weight when dried 34%) of the title compoundas a yellow solid: mp 152-154° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 4.69 (dd,J=9.6 Hz, J=6.7 Hz, 1H, CH at the chiral center), 4.02 (d, J=11.4 Hz,1H, CHH_(a)), 3.86 (d, J=11.4 Hz, 1H, CHH_(b)), 3.53-3.24 (m, 4H, CH₂),2.30-2.20 (m, 1H, CH), 2.04-1.72 (m, 3H, CH₂ and CH), 1.56 (s, 2H, Me);¹³C NMR (75 MHz, DMSO-d₆) δ 167.3, 163.1, 83.9, 57.2, 45.4, 37.8, 29.0,22.9, 21.6; IR (KBr) 3474, 1745 (C═O), 1687 (C═O), 1448, 1377, 1360,1308, 1227, 1159, 1062 cm⁻¹; [α]_(D) ²⁶+124.5° (c=1.3, chloroform);Anal. Calcd. for C₉H₁₂BrNO₃: C, 41.24, H, 4.61, N, 5.34. Found: C,41.46, H, 4.64, N, 5.32.

(2R)-3-Bromo-2-hydroxy-2-methylpropanoic Acid

A mixture of bromolactone (18.5 g, 71 mmol) in 300 mL of 24% HBr washeated at reflux for 1 h. The resulting solution was diluted with brine(200 mL), and was extracted with ethyl acetate (100 mL×4). The combinedextracts were washed with saturated NaHCO₃ (100 mL×4). The aqueoussolution was acidified with concentrated HCl to pH=1, which, in turn,was extracted with ethyl acetate (100 mL×4). The combined organicsolution was dried over Na₂SO₄, filtered through Celite®, and evaporatedin vacuo to dryness. Recrystallization from toluene afforded 10.2 g(86%) of the desired compound as colorless crystals: mp 107-109° C.; ¹HNMR (300 MHz, DMSO-d₆) δ 3.63 (d, J=10.1 Hz, 1H, CHH_(a)), 3.52 (d,J=10.1 Hz, 1H, CHH_(b)), 1.35 (s, 3H, Me); IR (KBr) 3434 (OH), 3300-2500(COOH), 1730 (C═O), 1449, 1421, 1380, 1292, 1193, 1085 cm⁻¹; [α]_(D)²⁶+10.5° (c=2.6, MeOH); Anal. Calcd. for C₄H₇BrO₃: C, 26.25, H, 3.86.Found: C, 26.28, H, 3.75.

Synthesis of(2R)-3-Bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide

Thionyl chloride (46.02 g, 0.39 mol) was added dropwise to a cooledsolution (less than 4° C.) of R-18 (51.13 g, 0.28 mol) in 300 mL of THFunder an argon atmosphere. The resulting mixture was stirred for 3 hunder the same condition. To this was added Et₃N (39.14 g, 0.39 mol) andstirred for 20 min under the same condition. After 20 min,5-amino-2-cyanobenzotrifluoride (40.0 g, 0.21 mol), 400 mL of THF wereadded and then the mixture was allowed to stir overnight at roomtemperature. The solvent was removed under reduced pressure to give asolid which was treated with 300 mL of H₂O, extracted with EtOAc (2×400mL). The combined organic extracts were washed with saturated NaHCO₃solution (2×300 mL) and brine (300 mL). The organic layer was dried overMgSO₄ and concentrated under reduced pressure to give a solid which waspurified from column chromatography using CH₂Cl₂/EtOAc (80:20) to give asolid. This solid was recrystallized from CH₂Cl₂/hexane to give 55.8 g(73.9%) of(2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide(R-19) as a light-yellow solid.

¹H NMR (CDCl₃/TMS) 61.66 (s, 3H, CH₃), 3.11 (s, 1H, OH), 3.63 (d, J=10.8Hz, 1H, CH₂), 4.05 (d, J=10.8 Hz, 1H, CH₂), 7.85 (d, J=8.4 Hz, 1H, ArH),7.99 (dd, J=2.1, 8.4 Hz, 1H, ArH), 8.12 (d, J=2.1 Hz, 1H, ArH), 9.04(bs, 1H, NH). Calculated Mass: 349.99, [M-H]⁻ 349.0. M.p.: 124-126° C.

Synthesis of(S)—N-(4-cyano-3-(trifluoromethyl)phenyl)-3-(4-cyano-3-fluorophenoxy)-2-hydroxy-2-methylpropanamide

A mixture of bromoamide((2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide,R-19 (2.0 g, 5.70 mmol) and anhydrous K₂CO₃ (2.4 g, 17.1 mmol) in 50 mLof acetone was heated to reflux for 2 h and then concentrated underreduced pressure to give a solid. The resulting solid was treated with2-fluoro-4-hydroxybenzonitrile (1.2 g, 8.5 mmol) and anhydrous K₂CO₃(1.6 g, 11.4 mmol) in 50 mL of 2-propanol and was heated to reflux for 3h, then concentrated under reduced pressure to give a solid. The residuewas treated with 100 mL of H₂O and then extracted with EtOAc (2×100 mL).The combined EtOAc extracts were washed with 10% NaOH (4×100 mL) andbrine, successively. The organic layer was dried over MgSO₄ and thenconcentrated under reduced pressure to give an oil which wascrystallized from CH₂Cl₂/hexane to give 0.5 g (23%) of(S)—N-(4-cyano-3-(trifluoromethyl)phenyl)-3-(4-cyano-3-fluorophenoxy)-2-hydroxy-2-methylpropanamideas a colorless solid.

¹H NMR (CDCl₃/TMS) δ 1.63 (s, 3H, CH₃), 3.34 (bs, 1H, OH), 4.08 (d,J=9.17 Hz, 1H, CH), 4.50 (d, J=9.17 Hz, 1H, CH), 6.74-6.82 (m, 2H, ArH),7.50-7.55 (m, 1H, ArH), 7.81 (d, J=8.50 Hz, 1H, ArH), 7.97 (q, J=2.03,8.50 Hz, 1H, ArH), 8.11 (d, J=2.03 Hz, 1H, ArH), 9.12 (s, 1H, NH).Calculated Mass: 407.1, [M+Na]⁺ 430.0. Mp: 124-125° C.

FIG. 6 schematically depicts some embodiments of synthetic processes toobtain compound of formula S-XXIII.

Example 11 Preclinical Anabolic and Androgenic Pharmacology of Compoundfor Formula S-XXIII in Intact and Castrate Male Rats

Anabolic and androgenic efficacy of compound of formula S-XXIIIadministered by daily oral gavage was tested. The S-isomer of compound(XXIII) was synthesized and tested as described herein.

Materials and Methods:

Male Sprague-Dawley rats weighing approximately 200 g were purchasedfrom Harlan Bioproducts for Science (Indianapolis, Ind.). The animalswere maintained on a 12-h light/dark cycle with food (7012C LM-485Mouse/Rat Sterilizable Diet, Harlan Teklad, Madison, Wis.) and wateravailable ad libitum. The animal protocol was reviewed and approved.

The test article for this study was weighed and dissolved in 10% DMSO(Fischer) diluted with PEG 300 (Acros Organics, N.J.) for preparation ofthe appropriate dosage concentrations. The animals were housed in groupsof 2 to 3 animals per cage. Animals were randomly assigned to one ofseven groups consisting of 4 to 5 animals per group. Control groups(intact and ORX) were administered vehicle daily. Compound of formulaS-XXIII was administered via oral gavage at doses of 0.01, 0.03, 0.1,0.3, 0.75, and 1 mg/day to both intact and ORX groups. Whereappropriate, animals were castrated on day one of the study. Treatmentwith compound of formula S-XXIII began nine days post ORX and wasadministered daily via oral gavage for fourteen days.

The animals were sacrificed under anesthesia (ketamine/xyalzine, 87:13mg/kg) and body weights were recorded. In addition, ventral prostate,seminal vesicles, and levator ani muscle were removed, individuallyweighed, normalized to body weight, and expressed as a percentage ofintact control. Student's T-test was used to compare individual dosegroups to the intact control group. Significance was defined a priori asa P-value <0.05. Ventral prostate and seminal vesicle weights wereevaluated as a measure of androgenic activity, whereas levator animuscle weight was evaluated as a measure of anabolic activity. Blood wascollected from the abdominal aorta, centrifuged, and sera were frozen at−80° C. prior to determination of serum hormone levels. Serumluteinizing hormone (LH) and follicle stimulating hormone (FSH)concentrations were determined.

Results:

A series of dose-response studies in intact and castrated rats in orderto evaluate the potency and efficacy of compound of formula S-XXIII inboth androgenic (prostate and seminal vesicles) and anabolic (levatorani muscle) tissue was conducted. In intact animals, compound of formulaS-XXIII treatment resulted in decreases in the weight of both prostateand seminal vesicles while the levator ani muscle weight wassignificantly increased. Levator ani muscle weight following compound offormula S-XXIII treatment were 116%±7%, 134%±8%, 134%±21%, 134%±11%,142%±10%, and 147%±10% of intact controls, following treatment with0.01, 0.03, 0.1, 0.3, 0.75, and 1.0 mg/day dose groups, respectively.The prostate weights were 98%±21%, 99%±8%, 85%±18%, 98%±22%, 126%±17%,and 126%±17% of intact controls, following treatment with 0.01, 0.03,0.1, 0.3, 0.75, and 1 mg/day, respectively. Similarly seminal vesicleweight was 115%±12%, 109%±17%, 106%±13%, 121%±11%, 157%±5%, and 136%±3%of intact controls following treatment with 0.01, 0.03, 0.1, 0.3, 0.75,and 1 mg/day, respectively. The results are graphically presented inFIG. 7. These results are significant since current androgen therapiesare contraindicated in some patient populations due to the proliferativeandrogenic effects in prostate and breast tissues. However, manypatients in these populations could benefit from the anabolic actions ofandrogens in muscle and bone. Since compound of formula S-XXIIIexhibited tissue selective anabolic effects, it may be possible to treatpatient groups in which androgens were contraindicated in the past.

In castrated (ORX) animals, prostate weights following compound offormula S-XXIII treatment were 24%±4%, 37%±9%, 50%±11%, 88%±16%,132%±16%, and 118±12% of intact controls following doses of 0, 0.01,0.03, 0.1, 0.3, 0.75, and 1 mg/day, respectively. Similarly, seminalvesicle weights were 15%±2%, 25%±9%, 67%±20%, 113%±6%, 155%±16%, and160%±7% of intact controls, following doses of 0, 0.01, 0.03, 0.1, 0.3,0.75, and 1 mg/day, respectively. Significant increases were seen inlevator ani muscle weights in all dose groups, when compared to intactcontrols. The levator ani muscle weights were 71%±4%, 101%±15%,125%±20%, 126%±14%, 151±9%, and 143±17% of intact controls correspondingto 0, 0.01, 0.03, 0.1, 0.3, 0.75, and 1.0 mg/day dose groups,respectively. The results are graphically presented in FIG. 8.

One unexpected finding was that administration of only 0.03 mg/day wasable to fully restore levator ani muscle weight.

Comparable administration of testosterone propionate (TP) andS-3-(4-acetylaminophenoxy)-2-hydroxy-2-methyl-N-(4-nitro-3-trifluoromethylphenyl)propionamide,maximally stimulated the levator ani muscle weight to 104% and 101%,respectively, indicating the significantly enhanced efficacy and potencyof compound of formula S-XXIII. Taken together, these data show thatcompound of formula S-XXIII restores lost muscle mass, which in someembodiments, finds valuable application in patients with sarcopenia orcachexia, or other wasting diseases or disorders. Additionally, theantiproliferative effects of compound of formula S-XXIII on the prostatemay allow some patient populations, in which androgens are currentlycontraindicated, access to anabolic agents. E_(max) values were obtainedand were 147%±10%, 188%±135%, and 147%±10% for prostate, seminalvesicles, and levator ani, respectively. The ED₅₀ in prostate, seminalvesicles, and levator ani was 0.21±0.04, 0.2±0.04, and 0.03±0.01 mg/day,respectively. These results are graphically depicted in FIG. 9.

Example 12 Synthesis of Compound of Formula S-XXIV) (FIG. 10)

(2R)-1-Methacryloylpyrrolidin-2-carboxylic Acid

D-Proline, 14.93 g, 0.13 mol) was dissolved in 71 mL of 2 N NaOH andcooled in an ice bath; the resulting alkaline solution was diluted withacetone (71 mL). An acetone solution (71 mL) of methacryloyl chloride(13.56 g, 0.13 mol) and 2 N NaOH solution (71 mL) were simultaneouslyadded over 40 min to the aqueous solution of D-proline in an ice bath.The pH of the mixture was kept at 10-11° C. during the addition of themethacryloyl chloride. After stirring (3 h, room temperature), themixture was evaporated in vacuo at a temperature at 35-45° C. to removeacetone. The resulting solution was washed with ethyl ether and wasacidified to pH 2 with concentrated HCl. The acidic mixture wassaturated with NaCl and was extracted with EtOAc (100 mL×3). Thecombined extracts were dried over Na₂SO₄, filtered through Celite®, andevaporated in vacuo to give the crude product as a colorless oil.Recrystallization of the oil from ethyl ether and hexanes afforded 16.2g (68%) of the desired compound as colorless crystals: mp 102-103° C.(lit. mp 102.5-103.5° C.); the NMR spectrum of this compounddemonstrated the existence of two rotamers of the title compound. ¹H NMR(300 MHz, DMSO-d₆) δ 5.28 (s) and 5.15 (s) for the first rotamer, 5.15(s) and 5.03 (s) for the second rotamer (totally 2H for both rotamers,vinyl CH₂), 4.48-4.44 for the first rotamer, 4.24-4.20 (m) for thesecond rotamer (totally 1H for both rotamers, CH at the chiral canter),3.57-3.38 (m, 2H, CH₂), 2.27-2.12 (1H, CH), 1.97-1.72 (m, 6H, CH₂, CH,Me); ¹³C NMR (75 MHz, DMSO-d₆) δ for major rotamer 173.3, 169.1, 140.9,116.4, 58.3, 48.7, 28.9, 24.7, 19.5: for minor rotamer 174.0, 170.0,141.6, 115.2, 60.3, 45.9, 31.0, 22.3, 19.7; IR (KBr) 3437 (OH), 1737(C═O), 1647 (CO, COOH), 1584, 1508, 1459, 1369, 1348, 1178 cm⁻¹; [α]_(D)²⁶+80.8° (c=1, MeOH); Anal. Calcd. for C₉H₁₃NO₃: C, 59.00, H, 7.15, N,7.65. Found: C, 59.13, H, 7.19, N, 7.61.

(3R,8aR)-3-Bromomethyl-3-methyl-tetrahydro-pyrrolo[2,1-c][1,4]oxazine-1,4-dione

A solution of NBS (23.5 g, 0.132 mol) in 100 mL of DMF was addeddropwise to a stirred solution of the (methyl-acryloyl)-pyrrolidine(16.1 g, 88 mmol) in 70 mL of DMF under argon at room temperature, andthe resulting mixture was stirred 3 days. The solvent was removed invacuo, and a yellow solid was precipitated. The solid was suspended inwater, stirred overnight at room temperature, filtered, and dried togive 18.6 g (81%) (smaller weight when dried 34%) of the title compoundas a yellow solid: mp 152-154° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 4.69 (dd,J=9.6 Hz, J=6.7 Hz, 1H, CH at the chiral center), 4.02 (d, J=11.4 Hz,1H, CHH_(a)), 3.86 (d, J=11.4 Hz, 1H, CHH_(b)), 3.53-3.24 (m, 4H, CH₂),2.30-2.20 (m, 1H, CH), 2.04-1.72 (m, 3H, CH₂ and CH), 1.56 (s, 2H, Me);¹³C NMR (75 MHz, DMSO-d₆) δ 167.3, 163.1, 83.9, 57.2, 45.4, 37.8, 29.0,22.9, 21.6; IR (KBr) 3474, 1745 (C═O), 1687 (C═O), 1448, 1377, 1360,1308, 1227, 1159, 1062 cm⁻¹; [α]_(D) ²⁶+124.5° (c=1.3, chloroform);Anal. Calcd. for C₉H₁₂BrNO₃: C, 41.24, H, 4.61, N, 5.34. Found: C,41.46, H, 4.64, N, 5.32.

(2R)-3-Bromo-2-hydroxy-2-methylpropanoic Acid

A mixture of bromolactone (18.5 g, 71 mmol) in 300 mL of 24% HBr washeated at reflux for 1 h. The resulting solution was diluted with brine(200 mL), and was extracted with ethyl acetate (100 mL×4). The combinedextracts were washed with saturated NaHCO₃ (100 mL×4). The aqueoussolution was acidified with concentrated HCl to pH=1, which, in turn,was extracted with ethyl acetate (100 mL×4). The combined organicsolution was dried over Na₂SO₄, filtered through Celite®, and evaporatedin vacuo to dryness. Recrystallization from toluene afforded 10.2 g(86%) of the desired compound as colorless crystals: mp 107-109° C.; ¹HNMR (300 MHz, DMSO-d₆) δ 3.63 (d, J=10.1 Hz, 1H, CHH_(a)), 3.52 (d,J=10.1 Hz, 1H, CHH_(b)), 1.35 (s, 3H, Me); IR (KBr) 3434 (OH), 3300-2500(COOH), 1730 (C═O), 1449, 1421, 1380, 1292, 1193, 1085 cm⁻¹; [α]_(D)²⁶+10.5° (c=2.6, MeOH); Anal. Calcd. for C₄H₇BrO₃: C, 26.25, H, 3.86.Found: C, 26.28, H, 3.75.

Synthesis of(2R)-3-bromo-N-(3-chloro-4-cyanophenyl)-2-hydroxy-2-methylpropanamide

Thionyl chloride (7.8 g, 65.5 mmol) was added dropwise to a cooledsolution (less than 4° C.) of (R)-3-bromo-2-hydroxy-2-methylpropanoicacid (9.0 g, 49.2 mol) in 50 mL of THF under an argon atmosphere. Theresulting mixture was stirred for 3 h under the same condition. To thiswas added Et₃N (6.6 g, 65.5 mol) and stirred for 20 min under the samecondition. After 20 min, 4-amino-2-chlorobenzonitrile (5.0 g, 32.8 mmol)and 100 mL of THF were added and then the mixture was allowed to stirovernight at room temperature. The solvent was removed under reducedpressure to give a solid which was treated with 100 mL of H₂O, extractedwith EtOAc (2×150 mL). The combined organic extracts were washed withsaturated NaHCO₃ solution (2×100 mL) and brine (300 mL), successively.The organic layer was dried over MgSO₄ and concentrated under reducedpressure to give a solid which was purified from column chromatographyusing EtOAc/hexane (50:50) to give 7.7 g (49.4%) of target compound as abrown solid.

¹H NMR (CDCl₃/TMS) δ 1.7 (s, 3H, CH₃), 3.0 (s, 1H, OH), 3.7 (d, 1H, CH),4.0 (d, 1H, CH), 7.5 (d, 1H, ArH), 7.7 (d, 1H, ArH), 8.0 (s, 1H, ArH),8.8 (s, 1H, NH). MS: 342.1 (M+23). Mp 129° C.

Synthesis of(S)—N-(3-chloro-4-cyanophenyl)-3-(4-cyanophenoxy)-2-hydroxy-2-methylpropanamide

A mixture of bromoamide (2.0 g, 6.3 mmol), anhydrous K₂CO₃ (2.6 g, 18.9mmol) in 50 mL of acetone was heated to reflux for 2 h and thenconcentrated under reduced pressure to give a solid. The resulting solidwas treated with 4-cyanophenol (1.1 g, 9.5 mmol) and anhydrous K₂CO₃(1.7 g, 12.6 mmol) in 50 mL of 2-propanol was heated to reflux for 3 hand then concentrated under reduced pressure to give a solid. Theresidue was treated with 100 mL of H₂O and then extracted with EtOAc(2×100 mL). The combined EtOAc extracts were washed with 10% NaOH (4×100mL) and brine, successively. The organic layer was dried over MgSO₄ andthen concentrated under reduced pressure to give an oil which waspurified by column chromatography using EtOAc/hexane (50:50) to give asolid. The solid was recrystallized from CH₂Cl₂/hexane to give 1.4 g(61.6%) of(S)—N-(3-chloro-4-cyanophenyl)-3-(4-cyanophenoxy)-2-hydroxy-2-methylpropanamideas a colorless solid.

¹H NMR (CDCl₃/TMS) δ 1.61 (s, 3H, CH₃), 3.25 (s, 1H, OH), 4.06 (d,J=9.15 Hz, 1H, CH), 4.50 (d, J=9.15 Hz, 1H, CH), 6.97-6.99 (m, 2H, ArH),7.53-7.59 (m, 4H, ArH), 7.97 (d, J=2.01 Hz, 1H, ArH), 8.96 (s, 1H, NH).Calculated Mass: 355.1, [M+Na]⁺ 378.0. Mp: 103-105° C.

Example 13 Preclinical Anabolic and Androgenic Pharmacology of S-XXIV inIntact and Castrate Male Rats

Anabolic and androgenic efficacy of compound of formula S-XXIVadministered by daily oral gavage was tested. The S-isomer of compoundS-XXIV was synthesized and tested as described herein.

Materials and Methods:

Male Sprague-Dawley rats weighing approximately 200 g were purchasedfrom Harlan Bioproducts for Science (Indianapolis, Ind.). The animalswere maintained on a 12-h light/dark cycle with food (7012C LM-485Mouse/Rat Sterilizable Diet, Harlan Teklad, Madison, Wis.) and wateravailable ad libitum. The animal protocol was reviewed and approved. Theanabolic and androgenic activity of compound of formula S-XXIV in intactanimals was tested, as well as a dose response evaluation in acutelyorchidectomized (ORX) animals. Regenerative effects of the compound offormula S-XXIV in chronically (9 days) ORX rats were similarlyevaluated.

The test article for this study was weighed and dissolved in 10% DMSO(Fischer) diluted with PEG 300 (Acros Organics, N.J.) for preparation ofthe appropriate dosage concentrations. The animals were housed in groupsof 2 to 3 animals per cage. Animals were randomly assigned to one ofseven groups consisting of 4 to 5 animals per group. Control groups(intact and ORX) were administered vehicle daily. Compound of formulaS-XXIV was administered via oral gavage at doses of 0.01, 0.03, 0.1,0.3, 0.75, and 1 mg/day to both intact and ORX groups. Whereappropriate, animals were castrated on day one of the study. Treatmentwith compound of formula S-XXIV began nine days post ORX and wasadministered daily via oral gavage for fourteen days.

The animals were sacrificed under anesthesia (ketamine/xyalzine, 87:13mg/kg) and body weights were recorded. In addition, ventral prostate,seminal vesicles, and levator ani muscle were removed, individuallyweighed, normalized to body weight, and expressed as a percentage ofintact control. Student's T-test was used to compare individual dosegroups to the intact control group. Significance was defined a priori asa P-value <0.05. Ventral prostate and seminal vesicle weights wereevaluated as a measure of androgenic activity, whereas levator animuscle weight was evaluated as a measure of anabolic activity. Blood wascollected from the abdominal aorta, centrifuged, and sera were frozen at−80° C. prior to determination of serum hormone levels. Serumluteinizing hormone (LH) and follicle stimulating hormone (FSH)concentrations were determined.

Results:

A series of dose-response studies in intact and castrated rats in orderto evaluate the potency and efficacy of compound of formula S-XXIV inboth androgenic (prostate and seminal vesicles) and anabolic (levatorani muscle) tissue was conducted. In intact animals, compound of formulaS-XXIV treatment resulted in decreases in the weight of both prostateand seminal vesicles while the levator ani muscle weight wassignificantly increased. Levator ani muscle weight following compound offormula S-XXIV treatment were 107%±5%, 103%±7%, 97%±7%, 103%±5%,118%±7%, and 118%±7% of intact controls following doses of 0.01, 0.03,0.1, 0.3, 0.75, and 1 mg/day, respectively. The prostate weights were103%±10%, 99%±10%, 58%±10%, 58%±15%, 65%±20%, and 77%±23% of intactcontrols following doses of 0.01, 0.03, 0.1, 0.3, 0.75, and 1 mg/day,respectively. These results are significant since current androgentherapies are contraindicated in some patient populations due to theproliferative androgenic effects in prostate and breast tissues.However, many patients in these populations could benefit from theanabolic actions of androgens in muscle and bone. Since compound offormula S-XXIV exhibited tissue selective anabolic effects, it may bepossible to treat patient groups in which androgens were contraindicatedin the past.

In castrated, ORX animals, prostate weights following compound offormula S-XXIV treatment were 12%±2%, 17%±6%, 31%±3%, 43%±15%, 54%±17%,58%±10%, and 73%±12% of intact controls following doses of 0, 0.01,0.03, 0.1, 0.3, 0.75, and 1 mg/day, respectively (FIG. 11). Similarly,seminal vesicle weights were 10%±2%, 10%±3%, 13%±4%, 21%±6%, 43%±8%,51%±9%, and 69%±14% of intact controls following doses of 0, 0.01, 0.03,0.1, 0.3, 0.75, and 1 mg/day, respectively (FIG. 11). Significantincreases were seen in levator ani muscle weights in all dose groups,when compared to intact controls. The levator ani muscle weights were40%±5%, 52%±8%, 67%±9%, 98%±10%, 103%±12%, 105%±12% and 110%±17% ofintact controls corresponding to 0, 0.01, 0.03, 0.1, 0.3, 0.75, and 1.0mg/day dose groups, respectively (FIG. 11).

Testosterone propionate (TP) andS-3-(4-acetylaminophenoxy)-2-hydroxy-2-methyl-N-(4-nitro-3-trifluoromethylphenyl)propionamide(S-4), maximally stimulated the levator ani muscle weight to 104% and101%, respectively. These data show that compound of formula S-XXIVexhibited significantly greater efficacy and potency than either TP orS-4. As a whole, these data show that compound of formula S-XXIV is ableto stimulate muscle growth in the presence or absence of testosteronewhile exerting anti-proliferative effects on the prostate. These datashow that the compound of formula S-XXIV restores lost muscle mass inpatients with sarcopenia or cachexia. Additionally, theantiproliferative effects of the compound of formula S-XXIV on theprostate may allow some patient populations, in which androgens arecurrently contraindicated, access to anabolic agents.

Anabolic ratios were derived comparing muscle/prostate weight incastrated rats. Values obtained were 3.02, 2.13, 2.27, 1.90, 1.83 and1.51 following doses of 0.01, 0.03, 0.1, 0.3, 0.75 and 1 mg/day,respectively.

Animals receiving 1 mg/day of compound of formula S-XXIV exhibited aprostate weight of 77%±23% and levator ani muscle weight of 118%±7% ofintact control values, respectively. Compound of formula S-XXIVmaintained prostate weight following orchidectomy at 73±12% of intactcontrols and levator ani muscle weight at 110±17% of intact controls. Aderived dose of 0.1 mg/day of compound of formula S-XXIV would restorelevator ani muscle weight to 100%, while such dose would only restore43±15% prostate weight.

Example 14 Synthesis of Compound of Formula S-XXV (FIG. 12)

(2R)-1-Methacryloylpyrrolidin-2-carboxylic Acid

D-Proline, 14.93 g, 0.13 mol) was dissolved in 71 mL of 2 N NaOH andcooled in an ice bath; the resulting alkaline solution was diluted withacetone (71 mL). An acetone solution (71 mL) of methacryloyl chloride(13.56 g, 0.13 mol) and 2 N NaOH solution (71 mL) were simultaneouslyadded over 40 min to the aqueous solution of D-proline in an ice bath.The pH of the mixture was kept at 10-11° C. during the addition of themethacryloyl chloride. After stirring (3 h, room temperature), themixture was evaporated in vacuo at a temperature at 35-45° C. to removeacetone. The resulting solution was washed with ethyl ether and wasacidified to pH 2 with concentrated HCl. The acidic mixture wassaturated with NaCl and was extracted with EtOAc (100 mL×3). Thecombined extracts were dried over Na₂SO₄, filtered through Celite®, andevaporated in vacuo to give the crude product as a colorless oil.Recrystallization of the oil from ethyl ether and hexanes afforded 16.2g (68%) of the desired compound as colorless crystals: mp 102-103° C.(lit. mp 102.5-103.5° C.); the NMR spectrum of this compounddemonstrated the existence of two rotamers of the title compound. ¹H NMR(300 MHz, DMSO-d₆) δ 5.28 (s) and 5.15 (s) for the first rotamer, 5.15(s) and 5.03 (s) for the second rotamer (totally 2H for both rotamers,vinyl CH₂), 4.48-4.44 for the first rotamer, 4.24-4.20 (m) for thesecond rotamer (totally 1H for both rotamers, CH at the chiral canter),3.57-3.38 (m, 2H, CH₂), 2.27-2.12 (1H, CH), 1.97-1.72 (m, 6H, CH₂, CH,Me); ¹³C NMR (75 MHz, DMSO-d₆) δ for major rotamer 173.3, 169.1, 140.9,116.4, 58.3, 48.7, 28.9, 24.7, 19.5: for minor rotamer 174.0, 170.0,141.6, 115.2, 60.3, 45.9, 31.0, 22.3, 19.7; IR (KBr) 3437 (OH), 1737(C═O), 1647 (CO, COOH), 1584, 1508, 1459, 1369, 1348, 1178 cm⁻¹; [α]_(D)²⁶+80.8° (c=1, MeOH); Anal. Calcd. for C₉H₁₃NO₃: C, 59.00, H, 7.15, N,7.65. Found: C, 59.13, H, 7.19, N, 7.61.

(3R,8aR)-3-Bromomethyl-3-methyl-tetrahydro-pyrrolo[2,1-c][1,4]oxazine-1,4-dione

A solution of NBS (23.5 g, 0.132 mol) in 100 mL of DMF was addeddropwise to a stirred solution of the (methyl-acryloyl)-pyrrolidine(16.1 g, 88 mmol) in 70 mL of DMF under argon at room temperature, andthe resulting mixture was stirred 3 days. The solvent was removed invacuo, and a yellow solid was precipitated. The solid was suspended inwater, stirred overnight at room temperature, filtered, and dried togive 18.6 g (81%) (smaller weight when dried 34%) of the title compoundas a yellow solid: mp 152-154° C.; ¹H NMR (300 MHz, DMSO-d₆) δ 4.69 (dd,J=9.6 Hz, J=6.7 Hz, 1H, CH at the chiral center), 4.02 (d, J=11.4 Hz,1H, CHH_(a)), 3.86 (d, J=11.4 Hz, 1H, CHH_(b)), 3.53-3.24 (m, 4H, CH₂),2.30-2.20 (m, 1H, CH), 2.04-1.72 (m, 3H, CH₂ and CH), 1.56 (s, 2H, Me);¹³C NMR (75 MHz, DMSO-d₆) δ 167.3, 163.1, 83.9, 57.2, 45.4, 37.8, 29.0,22.9, 21.6; IR (KBr) 3474, 1745 (C═O), 1687 (C═O), 1448, 1377, 1360,1308, 1227, 1159, 1062 cm⁻¹; [α]_(D) ²⁶+124.5° (c=1.3, chloroform);Anal. Calcd. for C₉H₁₂BrNO₃: C, 41.24, H, 4.61, N, 5.34. Found: C,41.46, H, 4.64, N, 5.32.

(2R)-3-Bromo-2-hydroxy-2-methylpropanoic Acid

A mixture of bromolactone (18.5 g, 71 mmol) in 300 mL of 24% HBr washeated at reflux for 1 h. The resulting solution was diluted with brine(200 mL), and was extracted with ethyl acetate (100 mL×4). The combinedextracts were washed with saturated NaHCO₃ (100 mL×4). The aqueoussolution was acidified with concentrated HCl to pH=1, which, in turn,was extracted with ethyl acetate (100 mL×4). The combined organicsolution was dried over Na₂SO₄, filtered through Celite®, and evaporatedin vacuo to dryness. Recrystallization from toluene afforded 10.2 g(86%) of the desired compound as colorless crystals: mp 107-109° C.; ¹HNMR (300 MHz, DMSO-d₆) δ 3.63 (d, J=10.1 Hz, 1H, CHH_(a)), 3.52 (d,J=10.1 Hz, 1H, CHH_(b)), 1.35 (s, 3H, Me); IR (KBr) 3434 (OH), 3300-2500(COOH), 1730 (C═O), 1449, 1421, 1380, 1292, 1193, 1085 cm⁻¹; [α]_(D)²⁶+10.5° (c=2.6, MeOH); Anal. Calcd. for C₄H₇BrO₃: C, 26.25, H, 3.86.Found: C, 26.28, H, 3.75.

Synthesis of(2R)-3-Bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide

Thionyl chloride (46.02 g, 0.39 mol) was added dropwise to a cooledsolution (less than 4° C.) of (R)-3-bromo-2-hydroxy-2-methylpropanoicacid (51.13 g, 0.28 mol) in 300 mL of THF under an argon atmosphere. Theresulting mixture was stirred for 3 h under the same condition. To thiswas added Et₃N (39.14 g, 0.39 mol) and stirred for 20 min under the samecondition. After 20 min, 5-amino-2-cyanobenzotrifluoride (40.0 g, 0.21mol), 400 mL of THF were added and then the mixture was allowed to stirovernight at room temperature. The solvent was removed under reducedpressure to give a solid which was treated with 300 mL of H₂O, extractedwith EtOAc (2×400 mL). The combined organic extracts were washed withsaturated NaHCO₃ solution (2×300 mL) and brine (300 mL). The organiclayer was dried over MgSO₄ and concentrated under reduced pressure togive a solid which was purified from column chromatography usingCH₂Cl₂/EtOAc (80:20) to give a solid. This solid was recrystallized fromCH₂Cl₂/hexane to give 55.8 g (73.9%) of(2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamideas a light-yellow solid.

¹H NMR (CDCl₃/TMS) δ 1.66 (s, 3H, CH₃), 3.11 (s, 1H, OH), 3.63 (d,J=10.8 Hz, 1H, CH₂), 4.05 (d, J=10.8 Hz, 1H, CH₂), 7.85 (d, J=8.4 Hz,1H, ArH), 7.99 (dd, J=2.1, 8.4 Hz, 1H, ArH), 8.12 (d, J=2.1 Hz, 1H,ArH), 9.04 (bs, 1H, NH). Calculated Mass: 349.99, [M-H]⁻ 349.0. M.p.:124-126° C.

Synthesis of(S)—N-(4-Cyano-3-(trifluoromethyl)phenyl)-3-(4-cyanophenoxy)-2-hydroxy-2-methylpropanamide

A mixture of bromoamide((2R)-3-bromo-N-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-2-methylpropanamide,50 g, 0.14 mol), anhydrous K₂CO₃ (59.04 g, 0.43 mol), 4-cyanophenol(25.44 g, 0.21 mol) in 500 mL of 2-propanol was heated to reflux for 3 hand then concentrated under reduced pressure to give a solid. Theresulting residue was treated with 500 mL of H₂O and then extracted withEtOAc (2×300 mL). The combined EtOAc extracts were washed with 10% NaOH(4×200 mL) and brine. The organic layer was dried over MgSO₄ and thenconcentrated under reduced pressure to give an oil which was treatedwith 300 mL of ethanol and an activated carbon. The reaction mixture washeated to reflux for 1 h and then the hot mixture was filtered throughCelite®. The filtrate was concentrated under reduced pressure to give anoil. This oil was purified by column chromatography using CH₂Cl₂/EtOAc(80:20) to give an oil which was crystallized from CH₂Cl₂/hexane to give33.2 g (59.9%) of(S)—N-(4-cyano-3-(trifluoromethyl)phenyl)-3-(4-cyanophenoxy)-2-hydroxy-2-methylpropanamideas a colorless solid (a cotton type).

¹H NMR (CDCl₃/TMS) δ 1.63 (s, 3H, CH₃), 3.35 (s, 1H, OH), 4.07 (d,J=9.04 Hz, 1H, CH), 4.51 (d, J=9.04 Hz, 1H, CH), 6.97-6.99 (m, 2H, ArH),7.57-7.60 (m, 2H, ArH), 7.81 (d, J=8.55 Hz, 1H, ArH), 7.97 (dd, J=1.95,8.55 Hz, 1H, ArH), 8.12 (d, J=1.95 Hz, 1H, ArH), 9.13 (bs, 1H, NH).Calculated Mass: 389.10, [M-H]⁻ 388.1. Mp: 92-94° C.

Example 15 Androgenic & Anabolic Activity in Intact and ORX Rats ofCompound of Formula S-XXV Materials and Methods

Male Sprague-Dawley rats weighing approximately 200 g were purchasedfrom Harlan Bioproducts for Science (Indianapolis, Ind.). The animalswere maintained on a 12-h light/dark cycle with food (7012C LM-485Mouse/Rat Sterilizable Diet, Harlan Teklad, Madison, Wis.) and wateravailable ad libitum. The animal protocol was reviewed and approved.Anabolic and androgenic activity of compound of formula S-XXV in intactanimals was evaluated, and the dose response in acutely orchidectomized(ORX) animals was evaluated as well. Regenerative effects of compoundfor formula S-XXV in chronically (9 days) ORX rats were also assessed.

The compound was weighed and dissolved in 10% DMSO (Fischer) dilutedwith PEG 300 (Acros Organics, N.J.) for preparation of the appropriatedosage concentrations. The animals were housed in groups of 2 to 3animals per cage. Intact and ORX animals were randomly assigned to oneof seven groups consisting of 4 to 5 animals per group. Control groups(intact and ORX) were administered vehicle daily. Compound of formulaS-XXV was administered via oral gavage at doses of 0.01, 0.03, 0.1, 0.3,0.75, and 1 mg/day to both intact and ORX groups.

Castrated animals (on day one of the study) were randomly assigned todose groups (4-5 animals/group) of 0.01, 0.03, 0.1, 0.3, 0.75, and 1mg/day, for dose-response evaluation. Dosing began nine days post ORXand was administered daily via oral gavage for fourteen days. Theanimals were sacrificed under anesthesia (ketamine/xyalzine, 87:13mg/kg) after a 14-day dosing regimen, and body weights were recorded. Inaddition, ventral prostate, seminal vesicles, and levator ani musclewere removed, individually weighed, normalized to body weight, andexpressed as a percentage of intact control. Student's T-test was usedto compare individual dose groups to the intact control group.Significance was defined a priori as a P-value <0.05. As a measure ofandrogenic activity, ventral prostate and seminal vesicle weights wereevaluated, whereas levator ani muscle weight was evaluated as a measureof anabolic activity. Blood was collected from the abdominal aorta,centrifuged, and sera were frozen at −80° C. prior to determination ofserum hormone levels. Serum lutenizing hormone (LH) and folliclestimulating hormone (FSH) concentrations were determined.

Results

Prostate weights following compound of formula S-XXV treatment were111%±21%, 88%±15%, 77%±17%, 71%±16%, 71%±10%, and 87%±13% of intactcontrols following doses of 0.01, 0.03, 0.1, 0.3, 0.75, and 1 mg/day,respectively. Similarly, seminal vesicle weights decreased to 94%±9%,77%±11%, 80%±9%, 73%±12%, 77%±10%, and 88%±14% of intact controlsfollowing doses of 0.01, 0.03, 0.1, 0.3, 0.75, and 1 mg/day,respectively. Significant increases were seen in levator ani muscleweights of sham animals, however, in all dose groups, when compared tointact controls. The levator ani muscle weights were 120%±12%, 116%±7%,128%±7%, 134%±7%, 125%±9%, and 146%±17% of intact controls correspondingto 0.01, 0.03, 0.1, 0.3, 0.75, and 1.0 mg/day dose groups, respectively.

Compound of formula S-XXV partially maintained prostate weight followingorchidectomy. Prostate weight in vehicle treated ORX controls decreasedto 5%±1% of intact controls. At doses of 0.01, 0.03, 0.1, 0.3, 0.75, and1.0 mg/day, compound of formula S-XXV maintained prostate weights at8%±2%, 20%±5%, 51%±19%, 56%±9%, 80%±28%, and 74±12.5% of intactcontrols, respectively. In castrated controls, seminal vesicle weightdecreased to 13%±2% of intact controls. Compound of formula S-XXVpartially maintained seminal vesicle weights in ORX animals. Seminalvesicle weights from drug treated animals were 12%±4%, 17%±5%, 35%±10%,61%±15%, 70%±14%, and 80%±6% of intact controls, following doses of0.01, 0.03, 0.1, 0.3, 0.75, and 1.0 mg/day, respectively. In ORXcontrols the levator ani muscle weight decreased to 55%±7% of intactcontrols. We observed an anabolic effect in the levator ani muscle ofcompound of formula S-XXV treated animals. Compound of formula S-XXVfully maintained levator ani muscle weights at doses >0.1 mg/day.Doses >0.1 mg/day resulted in significant increases in levator aniweight compared to that observed in intact controls. Levator ani muscleweights as a percentage of intact controls were 59%±6%, 85%±9%,112%±10%, 122%±16%, 127±12%, and 129.66±2% for the 0.01, 0.03, 0.1, 0.3,0.75, and 1.0 mg/day dose groups, respectively. E_(max) and ED₅₀ valueswere determined in each tissue by nonlinear regression analysis inWinNonlin® and presented in FIG. 13. E_(max) values were 83%±25%,85%±11%, and 131%±2% for prostate, seminal vesicles, and levator ani,respectively. The ED₅₀ in prostate, seminal vesicles, and levator aniwas 0.09±0.07, 0.17±0.05, and 0.02±0.01 mg/day, respectively.

Example 16 Studies of Compound of Formula S-XXIII on Knockout Mice

Dystrophin (DMD) homozygous null nice (−/−) and utrophin (UTRN)heterozygous mice (+/−) were obtained from JAX labs. The animals wereused to breed DMD (−/−) UTRN (+/+) and DMD (−/−) UTRN (−/−) mice.

When mice attained 4-6 weeks of age, male mice were castrated andtreated as indicated in Table 8.

TABLE 8 Study Design Group No. Mice Treatment Duration N 1 DMD (−/−)UTRN (−/−) Vehicle 12 weeks 8 2 DMD (−/−) UTRN (−/−) 10 mg/kg/day S-XXV12 weeks 8 3 DMD (−/−) UTRN (−/−) 10 mg/kg/day S-XXIII 12 weeks 8 4 DMD(−/−) UTRN (−/−) 10 mg/kg/day S-XXIV 12 weeks 8 5 Wildtype Vehicle 12weeks 8 6 Wildtype 10 mg/kg/day S-XXV 12 weeks 8 7 Wildtype 10 mg/kg/dayS-XXIII 12 weeks 8 8 Wildtype 10 mg/kg/day S-XXIV 12 weeks 8 9 DMD (−/−)UTRN (−/−) Vehicle till death (~20 weeks) 8 10 DMD (−/−) UTRN (−/−) 10mg/kg/day S-XXV till death (~20 weeks) 8 11 DMD (−/−) UTRN (−/−) 10mg/kg/day S-XXIII till death (~20 weeks) 8 12 DMD (−/−) UTRN (−/−) 10mg/kg/day S-XXIV till death (~20 weeks) 8 13 DMD (−/−) UTRN (+/+)Vehicle 12 weeks 8 14 DMD (−/−) UTRN (+/+) 10 mg/kg/day S-XXV 12 weeks 815 DMD (−/−) UTRN (+/+) 10 mg/kg/day S-XXIII 12 weeks 8 16 DMD (−/−)UTRN (+/+) 10 mg/kg/day S-XXIV 12 weeks 8

Compounds of formulas S-XXV, S-XXIII, and S-XXIV were used at 10 mpk/dayadministered subcutaneously. In FIG. 15, the labeling as ‘SARM’ or‘SARMs’ indicates cumulative data across groups 2, 3 and 4. One set ofUTRN wildtype mice (i.e., the mdx model or DMD (−/−) UTRN (+/+) mice)were also used (i.e., groups 13-16) to evaluate the effect of compoundof formula S-XXIII (and other SARMs) on Duchenne muscular dystrophythrough UTRN up-regulation.

Weekly body weight, MRI measurements, and grip strengths were measured(body weight: biweekly; MRI scan: once every 2 weeks or once every week;and grip strength: Once every 2 weeks or once every week).

Groups 1-8 and 13-16 were sacrificed after 12 weeks of treatment andvarious tissues were excised, weighed, and stored appropriately forfurther analysis. At sacrifice, blood was collected for serumbiochemical markers (ALT, AST, glucose, cholesterol, creatinine,creatine kinase, pyruvate, and others). Echocardiogram was performed inone set of knockout mice. As inflammation is considered as one of theprimary pathogenic mechanisms, a serum inflammatory markers panel wasevaluated. Organs (prostate, seminal vesicles, levator ani, soleus,gastrocnemious, heart, lungs, and liver) were weighed and stored forgene expression studies and histology. Levator ani, soleus, extensordigitorum longus (EDL), and gastrocnemious muscles were processed tomeasure the tension (if possible), histology and gene expression.

FIGS. 14A-14D show that DMD (−/−) UTRN (+/+) mice when treated withcompound of formula S-XXIII demonstrated a significant increase in bodyweight (FIG. 14A), lean [muscle] mass (FIG. 14C), and grip strength(FIG. 14D), and also a decrease in fat mass (FIG. 14B).

FIGS. 15A-15C show that double knock out mice when treated with vehiclesignificantly and rapidly lost their body weight, lean [muscle] mass,and grip strength. However, ‘SARMs’ (compounds of formulas S-XXV,S-XXIII, and S-XXIV; data shown is cumulative across groups 2-4) delayedthe deterioration of these measurements significantly. Further, ‘SARMs’enhanced the ability of these mice to be ambulatory.

DMD (−/−) UTRN (−/−) male mice were castrated and treated with vehicleor a compound of this invention at 10 mpk s.c. The number of weeks themice survived was recorded and expressed. The ‘SARM’ treated groupcombines the data for S-XXIII and S-XXV treated animals together.Animals from same litter are shown in FIGS. 16A and 16B. FIG. 16A(cumulative data for S-XXIII and S-XXV) and 16B (S-XXIII) show that dueto enhancement in body weight and lean [muscle] mass, the [castrated]DMD (−/−) UTRN (−/−) mice treated with the indicated compounds of theinvention lived longer by approximately 40-50% compared to [castrated]vehicle-treated control mice from the same litter, suggesting the resultis not due to genetic variation but drug efficacy. Cumulatively, thissuggests that patients with Duchenne muscular dystrophy treated withS-XXIII (or another SARM of this invention) are expected to not onlybenefit from improved growth and strength (e.g., improved physicalfunction and quality of life such as longer ambulation) but also maylive longer lives. A possible explanation for the survival benefit maybe seen in Example 17 below, as the compounds of this invention alsoimprove cardiac function in model of Duchenne muscular dystrophy. FIGS.19A and 19B and FIGS. 20A and 20B show that intact and castrated mdxmice, i.e., DMD (−/−) UTRN (+/+), were both effected by S-XXIIItreatment. The effects of S-XXIII reported above for castrated mdx micewere also seen in intact mdx mice. E.g., S-XXIII increased body weight(FIG. 19) and lean mass (FIG. 20) in castrated and intact mdx mice. Thissuggests that the therapeutic effects for Duchenne muscular dystrophywere not an artifact of the use of castrated animals to model thedisease.

Example 17 Echocardiography Test in Mdx Mice

FIG. 17: DMD (−/−) UTRN (+/+) (mdx mice; separate experiment fromExample 16) male mice were castrated and treated with vehicle or S-XXIII(10 mg/kg/day s.c.) for 10 weeks. Body weight, lean mass, and gripstrength were measured at the end of treatment period. Echocardiogramwas performed on all mice (n=4/group). Echo results are represented asbar graphs in FIG. 18.

Consistent with Example 16 and the scientific literature, mdx miceexperienced decreases in growth and strength. However, mice treated withcompound of formula S-XXIII had increased body weight, lean [muscle]mass, and grip strength, demonstrating the global therapeutic effects ofcompounds of this invention. Further, the mdx mice suffered from aorticstenosis and aortic valvular dysfunction. However, this disorder wasshown to be completely reversed by treatment with compound of formulaS-XXIII (FIGS. 18A-18D).

FIG. 18A also shows that the cardiac function was highly compromised inmdx mice (ejection fraction (EF; %)). However, the compromised cardiacfunction was reversed by compound of formula S-XXIII. Ejection fractionless than 55% is considered as cardiac failure. The mdx mice have EF of45%, while the treated mice have EF of 63%. The data show that compoundof formula S-XXIII strengthens cardiac muscle. FIG. 18B shows theeffects of S-XXIII on fractional shortening (FS) (%). Fractionalshortening is used as an estimate of myocardial contractility. However,this is only a guide and is very dependant on the loading factors whichaffect the contraction of the heart. If the ventricle does not fillnormally during diastole the FS % will be reduced. FS % is particularlysensitive to changes in afterload. An increase in systemic bloodpressure or an increase in myocardial stiffness will therefore reduce FS%. FS % can also be influenced by the heart rate. Excitement may resultin an increased FS % as a result of catecholamine release. Valvularheart disease will affect ventricular function before any change inmyocardial contractility occurs. For example, mitral regurgitation willresult in a decreased afterload because it acts as a let-off valveduring systole. In addition, if the valvular disease is severe enough tohave resulted in volume overload, preload may be increased. Thesefactors increase FS % by decreasing systolic dimensions and increasingdiastolic dimensions respectively. Once myocardial failure develops, FS% will fall. FIG. 18B demonstrates that mdx mice have decreased FS %consistent with heart failure which is reversed by S-XXIIIadministration. FIG. 18C shows the effects of S-XXIII on AV peakvelocity (aortic valve peak velocity) (mm/s). FIG. 18D shows the effectsof S-XXIII on AV peak pressure (mmHg). The dramatically increased AVpeak velocity and AV peak pressure indicates stenosis (narrowing orconstriction) of the aortic valve which was reversed by S-XXIIIadministration.

Example 18 S-XXIII Alters Lung Function in DMD Mice

Materials and Methods:

Saturated Oxygen Levels and Heart and Lung Rate:

Wildtype or DMD KO (DMD (−/−)) mice were either sham operated orcastrated and treated for 10 weeks. At the end of the experiment,respiratory rate (RR), heart rate (HR), and arterial oxygen content(SPO2%) were measured using STARR Life Sciences “MouseOx” with 6.2.1software version. All mice had hair removed from right inner/medialthigh with Nair@ cream. The mouse thigh sensor was placed in thehairless region with the receiver on the inside and the light source onthe outside of the leg. All recordings were taken over 2-3 minutes withthe highest SPO2% recorded for each mouse and the corresponding HR andRR at that particular SPO2% value. All mice were manually restrained bythe same lab member throughout all individual recordings.

CLAMS (Comprehensive Laboratory Animal Monitoring System):

Wildtype or DMD KO (DMD (−/−)) mice were either sham operated orcastrated and treated for 10 weeks. At the end of the experiment,animals were maintained in CLAMS for 2 days to continuously monitoringof oxygen consumption, energy expenditure, and mobility.

Flexivent:

Wildtype or DMD KO (DMD (−/−)) mice were either sham operated orcastrated and treated for 10 weeks. At the end of the experiment, inanesthetized (ketamine/xylazine, 180/10 mg kg⁻¹) and tracheotomizedmice, the peak airway resistance values in response to increasing dosesof inhaled methacholine (MeCh) were measured using the flexiVent® system(Scireq; Montreal, Canada) according to manufacturer's instructions.

Results

The results indicate that S-XXIII improves lung and cardiac function inDMD (−/−) mice. As shown in FIGS. 21A-21C, the arterial saturated oxygenlevels (SPO2) in DMD (−/−) animals treated with S-XXIII increasedcompared to (intact and) castrated vehicle-treated DMD (−/−) animals.While S-XXIII increased the saturated oxygen levels, it reduced theheart (HR) and breathing (RR) rates, indicative of better cardiac andlung function.

The CLAMS data shown in FIGS. 22A-22D and FIG. 23 demonstrate thatcastration reduced the oxygen consumption (VO2), a measure of energyexpenditure, and reduced mobility (i.e., activity) significantlycompared to intact animals. Moreover, the intact DMD (−/−) mice havevery low oxygen consumption and mobility compared to wildtype mice.These measurements were improved by S-XXIII indicative of a reversal inthe ability to move and also to maintain energy expenditure, suggestingimproved pulmonary functions, physical function, and quality of life ispossible for Duchenne muscular dystrophy patients.

The flexiVent® data (FIG. 24) indicate that treatment of castrated DMDKO (DMD (−/−)) mice with S-XXIII reduced the methacholine (MeCh)-inducedairway resistance significantly compared to vehicle-treated castratedDMD (−/−) mice. The variability in the maximum degree ofbronchoconstriction that can be induced by exogenous bronchoconstrictivesubstances such as methacholine could result from differences in theproperties or quantity of the airway smooth muscle (ASM), the load onthe muscle, or the thickness of the airway wall. Healthy muscle willhave lower airway resistance in response to bronchoconstrictive agents.S-XXIII-treated animals had lower airway resistance in response tomethacholine, which is indicative of healthier and stronger lung muscleand suggestive of the possibility to prevent or reverse respiratoryinsufficiency or respiratory failure in Duchenne muscular dystrophypatients.

Example 19 Circulating Androgens are Important for Survival

To test that the health of pre-pubertal boys rapidly deteriorates due toabsence of circulating androgens, dKO (DMD (−/−) UTRN (−/−)) mice weresham-operated or castrated. Since MDX mice survive comparably towild-type mice, dKO mice that live for only 15-20 weeks were chosen tomonitor survival. Body weight and lean mass were measured using MRI andtheir survival was monitored. Sham-operated (i.e., intact) dKO micemodestly gained body weight and lean mass by 15-25% from the initiationof the study and maintained this gain until the study was terminated byweek 6 (FIGS. 25A-25C). On the other hand, castrated dKO mice lost 20%body weight and lean mass (FIGS. 25A and 25B). Almost 66% of thecastrated dKO mice died within 6 weeks of the study initiation, whileonly 25% of the sham-operated mice died during the same period (FIGS.25C and 25D). This suggests that circulating androgens are important forthe maintenance of body weight and lean mass and are critical for thesurvival of extremely weak dKO DMD mice.

Example 20 SARMs Potently Activated the AR

To determine if AR ligands will rescue the detrimental phenotypeobserved due to absence of circulating androgen, the steroidal androgendihydrotestosterone (DHT) or nonsteroidal tissue-selective SARMs weretested. Several advantages such as nonsteroidal backbone, selectivity tomuscle and bone, lack of hepatotoxicity, and others favored thecontinued use of SARMs in the studies. Moreover, DHT can beenzymatically inactivated to weaker androgens or estrogens. A previousstudy demonstrated beneficial effects of SARMs in MDX mice (Cozzoli A,et al. Pharmacol Res. 2013, 72, 9-24). Three arylpropionamide SARMs(S-XXIII, S-XXV, and S-XXIV) that bind to the AR ligand binding domain(LBD) with K_(i) between 2 and 10 nM were tested in an ARtransactivation assay in HEK-293 cells. All three SARMs were extremelypotent at nM concentration in increasing AR transactivation, withS-XXIII being the most potent of the three tested (FIG. 26A).

The intracellular milieu of coactivators and corepressors regulate thepotency of nuclear receptor ligands. In order to evaluate whether themuscle cell environment will alter the efficacy of S-XXIII, atransactivation assay was performed with DHT and S-XXIII in the C2C12muscle cell line. While S-XXIII was extremely efficacious in activatingthe AR in C2C12 cells, DHT was potent but a much weaker agonist in C2C12cells than in HEK-293 cells (FIG. 26B). This result shows that S-XXIIIis potent and a very strong agonist in a muscle cell environment.

SARMs Increased Muscle Mass, Physical Function, and Body Weight ofWild-Type Mice.

C57BL/6 male mice (6 weeks old; n=6/group) were castrated to removecirculating androgens and treated subcutaneously with vehicle or 10mg/kg/day S-XXIII. S-XXIII significantly (p<0.001) increased body weightand lean mass starting after 1 week and maintained significant activityat the end of treatment (12 weeks) (FIG. 26C). Grip strength, which wasused as a measure of physical function, was significantly increased byS-XXIII from week 4 of treatment (FIG. 26C) compared to vehicle-treatedanimals. These results set the stage for the potential testing ofS-XXIII in preclinical models of DMD.

Example 21 S-XXIII Increased Lean Mass, Body Weight, and Grip Strengthin MDX Mice

DMD (−/−) UTRN (+/−) mice purchased from JAX labs were bred in-house toobtain the required male DMD (−/−) UTRN (+/+) (MDX) mice for thesestudies. Male mice of 4-6 weeks of age were castrated and treatedsubcutaneously with vehicle or 10 mg/kg/day S-XXIII. Body weight andcomposition by MRI were recorded weekly. Grip strength of fore- andhind-paws was recorded at the beginning of the study and at 6 and 12weeks of treatment. Similar to the wild-type mice, S-XXIII increasedbody weight and muscle mass starting from week 2 and the differencewidened over the duration of the study (FIG. 26D FIG. 26F). S-XXIIIincreased the lean mass by at least three-fold (20% vs 60%) compared tothe vehicle-treated animals. Consistent with the increase in lean mass,grip strength also increased in the S-XXIII-treated animals (FIG. 26DFIG. 26F). These results were reproduced with the other two SARMs, S-XXVand S-XXIV, indicating that these activities are not unique to S-XXIII.

As boys affected by DMD exhibit pathologic signs as early as 3-5 yearsof age when no circulating androgens can be detected, we performed theexperiment (FIG. 26D FIG. 26F) in castrated male mice to replicate thescenario in pre-pubertal boys. Subsequently, to mimic the effect ofS-XXIII in boys who have attained puberty, an experiment was performedin sham-operated intact male mice. S-XXIII significantly increased thelean mass by two weeks of treatment and maintained this increase untilsix weeks of treatment (FIG. 26G). However, this significancedisappeared by eight weeks of treatment (14 weeks of age), which couldbe potentially due to synthesis of endogenous androgens. Concurrent withthe increase in lean mass, grip strength also increased in intact micetreated with S-XXIII compared to the vehicle-treated mice (FIG. 26G).

Example 22 Gastrocnemius and Soleus Muscle from S-XXIII-Treated Mice hadLower Incidence of Centrally Nucleated Cells and Fibrosis

Materials and Methods:

All in vitro experiments were performed at least thrice and in vivoexperiments were performed with approximately 4-10 mice, depending onthe study.

AR Transactivation:

AR transactivation was performed as previously described (Narayanan R.et al. Cancer Res. (2010) 70(2), 842-851). Briefly, human AR cloned intoCMV vector backbone was used for the transactivation study. HEK-293 orC2C12 cells over-expressing the AR were plated at 120,000 cells per wellof a 24 well plate in DME containing 5% csFBS. The cells weretransfected using Lipofectamine (Invitrogen, Carlsbad, Calif.) with 0.25μg GRE-LUC, 0.02 μg CMV-LUC (renilla luciferase) and 25 ng of the AR.The cells were treated 24 hrs after transfection as indicated in thefigures and the luciferase assay performed 48 hrs after transfection.

Gastrocnemius and soleus muscle from castrated MDX mice treated withvehicle or S-XXIII for 12 weeks were formalin fixed, histologicallyprocessed, and stained with hematoxylin and eosin (H&E), Mason trichromefor collagen deposition and fibrosis, and Van Geison for elastin. One ofthe hallmarks of atrophied muscle is the presence of centrally nucleatedcells (Liu M et al. Mol Ther (2005) 11(2), 245-256). While normalhealthy muscle cells have nuclei in the periphery, muscle from DMDpatients and MDX mice have centrally located nuclei. H&E stained soleusand gastrocnemius sections from castrated MDX mice exhibited almost 100%centrally-nucleated cells, while the sections from wild-type mice hadfew or no centrally nucleated cells (FIGS. 26H and 26I). The number ofcentrally-nucleated cells was partially reduced by 30-40% (p<0.05) byS-XXIII, indicating a potential reversal of atrophy.

The extent of fibrosis was evaluated in Mason trichrome-stained soleusand gastrocnemius sections and a score of 0-3 was provided. Soleus andgastrocnemius muscle of vehicle-treated castrated MDX mice hadsubstantially higher fibrosis scores, close to 3 in several cases, whilethose from mice treated with S-XXIII had a significantly lower fibrosisscores (FIGS. 26J and 26K). The same trend was observed in the number ofnecrotic cells (FIG. 26J), while no differences in the number ofinflammatory cell infiltration (FIG. 26J) was observed between vehicle-and S-XXIII-treated muscle.

The extent of fibrosis was evaluated in Mason trichrome-stained lungs ofthe MDX mice. At the end of treatment, lung tissues were fixed andstained for collagen by trichrome staining. The sections were scoredbetween 1 and 3 based on the intensity of staining (FIG. 26L). Collagen(blue stain) staining, which is indicative of fibrosis, was modest insham-operated (i.e., intact) MDX mice (FIG. 26M) and MDX mice castratedand treated with S-XXIII (FIG. 26O), while the staining was intense inMDX mice castrated and treated with vehicle (FIG. 26O). This reductionin fibrosis by S-XXIII could be a reason for better lung function in theventilator and CLAMS studies. Castrated vehicle-treated MDX mice lungshad higher intensity stain and the stain was spread over larger area.Representative images are shown in the following slides.

Example 23 RNA-Sequencing Studies Indicate that S-XXIII and DystrophinRegulate Distinct Pathways in MDX Mice

To determine the mechanism of action of S-XXIII, RNA sequencing studieswere performed in gastrocnemius muscle from castrated vehicle-treatedwild-type mice, castrated vehicle- or S-XXIII-treated MDX mice(n=3/group). Dystrophin knock-down altered the expression of 417 genescompared to wild-type mice (FIG. 27C-FIG. 27E). Most up-regulated genesin MDX mice include prune-2, IGFN-1, Mettl21e, IGF-2, and Ahnak-2 andthe most down-regulated genes include Dusp-26, Gcsh, ppp1r1a, htra4, andDMD. Ingenuity pathway analysis (IPA) of the genes significantlyenriched in MDX mice shows that the top canonical signaling pathwayaltered is nNOS signaling (Tameyasu T, et al. Jpn J Physiol. 2004,54(6), 555-66) and creatinine biosynthesis, while the top upstreamregulators are all myoanabolic pathway markers such as DMD, MYOD1, andMYOG. Moreover, IPA showed that the biological functions of genesenriched in MDX mice are found in neurological diseases, respiratorydiseases, and skeletal and muscular disorder, and developmentaldisorder. These results indicate that there is a good correlationbetween the expression of these genes and the phenotype in MDX mice.

Genes regulated by S-XXIII in castrated MDX mice were compared to thatof castrated vehicle-treated MDX mice (FIG. 27A and FIG. 27B).Interestingly only 60 genes were differentially regulated by S-XXIII,which is a much smaller subset compared to the genes altered in prostateby AR ligands. The most up-regulated genes include Inmt, Kcng4, Amd1,Amd2, pla1a, while the most down-regulated genes include Casr, Grmd1b,Mybph, and MAPK8ip1. IPA analysis indicates that S-XXIII enriched genesbelong to two highly relevant canonical pathways, spermine andspermidine biosynthesis (Wei C, et al. Int J Mol Med. 2016; 37(1):39-46)which play an important role in skeletal muscle hypertrophy and atrophy(Chrisam M, et al. Autophagy, 2015, 11(12), 2142-52). The genes that areenriched by S-XXIII mediate the biological functions of connectivetissue disorder, skeletal muscle disorder, and developmental disorder.Interestingly, there was minimum to no overlap between genes regulatedby dystrophin knockdown and the genes regulated by S-XXIII treatment,indicating that S-XXIII does not function by reversing the pathwaysde-regulated by dystrophin knockdown. Consequently, S-XXIII and otherSARMs are likely to provide complementary or synergistic therapeuticeffects if combined with therapies that result in expression ofdystrophin or utrophin such as stem cell therapies, viral genetherapies, exon-skipping anti-sense oligomers, ribosomal read-through(i.e., mutation suppression), or utrophin up-regulators, and possiblyother emerging classes of DMD therapeutics.

MicroRNAs (miRNA) are non-coding RNAs that are 22-24 nucleotides longthat base pair with 3′UTR of mRNA to silence specific genes. SpecificmiRNAs are critical for the physiology and pathology of multipletissues. MiRNAs play a pivotal role in musculoskeletal equilibrium andpathogenesis. miRNA expression with a Nanostring (NanostringTechnologies Inc., Seattle, Wash. 98109) panel was determined, in thesame RNA preparation used for RNA-sequencing (FIG. 27F and FIG. 27G).Dystrophin knockdown modified the expression of 10 miRNAs, which werenot reversed by S-XXIII treatment (FIG. 27F). In addition, S-XXIII hadminimal effect on miRNA expression modifying the expression of only 4miRNAs (FIG. 27G).

Example 24 SARMs Increased Lean Mass, Physical Function, and Survival ofDMD (−/−) UTRN (−/−) Double Knockout Mice

Double-knockout (dKO) mice of dystrophin (DMD) and utrophin (UTRN, aprotein that compensates for lack of dystrophin) (i.e., DMD (−/−) UTRN(−/−)) present a phenotype more representative of the DMD in humans,including progressive worsening of symptoms, loss of ambulation at 12weeks, and early death by ˜20 weeks. Although these dKO mice reportedlysurvive to 20 weeks, vehicle-treated castrated double knockout mice inour studies survived only up to 9-12 weeks of age (5-8 weeks after studyinitiation), indicating that androgens might potentially contribute tothe increased survival of these mice (FIG. 25A-FIG. 25C). The ability ofS-XXIII to increase the muscle mass and physical function and survivalin these dKO mice was determined. Body weight, lean mass, and gripstrength of vehicle-treated castrated dKO mice all rapidly deteriorated(FIG. 28A-FIG. 28C). S-XXIII treatment of these mice increased all theseparameters above the baseline and maintained them until the end of thestudy. These effects were translated into long-term survival (FIG. 28D).Vehicle-treated castrated mice survived only between 7 and 10 weeks ofage (3-7 weeks after study initiation), while SARM-treated castratedmice survived longer with an approximately 50-70% increase in survivalcompared to the vehicle-treated mice.

The results clearly demonstrate a beneficial role of SARMs in DMD. Bothcastrated male DMD mice (mimic pre-puberty boys), and intact DMD mice(represent teens and adults) responded to S-XXIII and other SARMs.S-XXIII and other SARMs elicited these beneficial effects athistological and molecular level by reducing the number of centrallynucleated cells, fibrosis, and necrosis and significantly regulating theexpression of genes important for muscle hypertrophy.

Most interestingly SARMs treatment reversed cardiomyopathy in thesemice. Although cardiomyopathy has been cited as the primary cause ofdeath in DMD boys, aortic valvular disorder has not been identifiedpreclinically in MDX mice. This remarkable discovery could reflectandrogen depletion. A significant reduction was found in overall cardiacfunction with ejection fraction numbers comparable to cardiac failure incastrated MDX mice. It is possible that cardiomyopathy is due toweakening of cardiac muscle, which resulted in fibrosis of heart tissue.S-XXIII and other SARMs reversed anatomical, physiological, andhistological deficiencies to that observed in healthy reference control.

As the phenotype of MDX mice is mild, dKO mice that have a debilitatingphenotype similar to DMD boys were used to evaluate the effect ofS-XXIII on survival. Although the dKO mice are known to survive up to 20weeks of age, we observed that androgen depletion due to castration ledto premature death by 10-13 weeks of age. S-XXIII (and other SARMs) notonly improved the body weight, muscle mass, and grip strength, but alsosignificantly increased survival. This is the first study to demonstratea survival benefit with a therapeutic agent in a preclinical model ofDMD. The nNOS signaling pathway, which was deregulated in the studies inthe MDX mice, was shown to be affected in the MDX models, validating thegene expression data (Brenman J. E., et al. Cell. 1995, 82(5), 743-52;Chang W. J., et al. Proc Natl Acad Sci USA. 1996, 93(17), 9142-7).

The nNOS pathway was significantly down-regulated in the muscle of MDXmice, which was not reversed by S-XXIII. While S-XXIII did not reversethe genes altered by dystrophin knockdown, it regulated the expressionof genes in the spermine and spermidine pathways. Interestingly,spermine oxidase, a gene that maintains skeletal muscle and preventsatrophy was significantly increased by 2-3 fold by S-XXIII in castratedMDX mice (Bongers K. S., et al. Am J Physiol Endocrinol Metab. 2015,308(2), E144-58). Again, genes belonging to the spermidine pathway, aproduct of spermine oxidase, was also up-regulated by S-XXIII.Spermidine has been shown to be important for enhanced cell survival.These results provide a rationale to combine AR-targeted therapeuticswith exon-skipping molecules, utrophin up-regulators, myostatininhibitors, or other possible therapeutic agents in development for DMD.Since these signaling pathways target distinct proteins, we expect asynergy by combining them. With such positive effects on multipletissues and organs, we expect that AR ligands such as S-XXIII couldbecome an important treatment option for DMD patients. These moleculescould be used as stand-alone or in combination with other approved oremerging therapies. The magnitude of response observed in lung and heartare compelling enough to argue that these molecules could be very usefulto extend survival of pre-pubertal boys afflicted by DMD.

Materials and Methods:

All in vitro experiments were performed at least thrice and in vivoexperiments were performed with approximately 4-10 mice, depending onthe study.

Ar Transactivation:

Briefly, human AR cloned into CMV vector backbone was used for thetransactivation study. HEK-293 or C2C12 cells over-expressing the ARwere plated at 120,000 cells per well of a 24 well plate in DMEcontaining 5% csFBS. The cells were transfected using Lipofectamine(Invitrogen, Carlsbad, Calif.) with 0.25 μg GRE-LUC, 0.02 μg CMV-LUC(renilla luciferase) and 25 ng of the AR. The cells were treated 24 hafter transfection as indicated in the figures and the luciferase assayperformed 48 h after transfection. The EC₅₀ values were obtained fromfour parameter logistics curve.

MDX and dKO Animal Studies:

MDX and dKO mice were derived from breeding DMD (−/−) UTRN (+/−).Genotyping was performed in accordance to Jackson Laboratories protocolusing the recommended primers. Male mice (4-6 weeks old) were used forthe experiments. MRI measurements were performed as indicated in thefigure using the EchoMRI machine (Houston, Tex.). Grip strengthmeasurements (Columbus Instruments, Columbus, Ohio 43204) were madethrice each time with both front and rear paws and the highest valuefrom each animal was considered.

RNA-Sequencing:

Total RNA (1 μg) was enriched for poly-A+RNA using Ambion Dynabeads mRNADirect Micro Kit (Thermo Fisher Scientific, Waltham, Mass. 02451) andbarcoded libraries for sequencing were prepared using the LifeTechnologies RNAseq V2 Kit (Thermo Fisher Scientific, Waltham, Mass.02451) for Ion Torrent according to manufacturer's standard protocol.Libraries were amplified 14 cycles and the quality of each library waschecked on an Agilent Bioanalyzer DNA High Sensitivity Chip (AgilentTechnologies Inc., Santa Clara, Calif. 95051). The libraries were pooledbased on the concentration of each sample between 200-350 bp, purifiedon a Pippin Prep Gel (Sage Science, Inc., Beverly, Mass. 01915),quantified by the Agilent Bioanalyzer and sequenced on an Ion TorrentProton Sequencer (Thermo Fisher Scientific, Waltham, Mass. 02451).Sequencing was performed by University of Tennessee Health ScienceCenter (UTHSC) Molecular Resources Center.

Bioinformatics:

Fastq files were retrieved from the Ion Torrent Server at the UTHSCMolecular Resource Center. FastQC (Babraham Bioinformatics, England) wasrun in order to trim any bases who had a phred score <20. Once the fastqfiles were trimmed, they were aligned to the Mus musculus 9transcriptome using RNA-STAR. The alignment was able to match 85% of theraw reads on average. The same files were then mined for the total readcounts for each transcript. The read counts were normalized to thelowest total number of reads across the experiment to allow comparisonsacross each sample. Once normalized, the read counts were used tocalculate the average fold change and Student's t-test betweengroupings. Only transcripts that showed a fold change ≥1.5 and a p value≤0.05 were selected. Finally the false discovery rate was calculatedusing the Benjamini and Hochberg false discovery rate method. Onlytargets with a q value ≤0.05 were selected for the final transcriptlist. This list was uploaded to Ingenuity Pathway Analysis (Qiagen,Redwood City, Calif. 94063) for functional analysis.

FastQC:

http://www.bioinformatics.babraham.ac.uk/proiects/fastqc/

RNA-STAR:

http://www.ncbi.nlm.nih.gov/pubmed/23104886

CLAMS:

Prior to energy expenditure measurements, mice were weighed and totalfat and fat-free mass determined non-invasively using an EchoMRI-1100(EchoMRI™, Houston, Tex.). Mice were then individually housed in a homecage-style Comprehensive Laboratory Animal Monitoring System (CLAMS;Columbus Instruments, Columbus, Ohio) where they had free access to foodand water. Physical activity and respiratory gas exchange were monitoredover a 5-day period and values for each of the measured variablescalculated using CLAX software (Version 2.2.12; Columbus Instruments).The first 20 data collection cycles were excluded from analysis, as thiswas the pre-determined CLAMS acclimation phase. Energy expenditure wascalculated as VO₂ relative to fat-free mass, whereas respiratoryexchange ratios were calculated from the VO₂ and VCO₂ data. Physicalactivity was calculated as ambulatory movement, with the number ofconsecutive beam breaks across the X and Y cage axes indicating bouts ofactivity. Data for the light (rest) and dark (active) phases wereseparated and independently analyzed.

MiRNA Expression Analysis:

Microarray analysis on RNA prepared from mouse tissue was performedusing a panel of ˜800 miRNAs (NanoString Technologies, Seattle, Wash.,USA). In brief, total RNA was mixed with pairs of capture and reporterprobes, hybridized on the nCounter Prep Station, and purified complexeswere quantified on the nCounter Digital Analyzer. To account fordifferences in hybridization and purification, data were normalized tothe average counts for all control spikes in each sample and analyzedwith nSolver software.

Echocardiography:

Using a Vevo 2100 Imaging System (Visualsonics, Toronto, Canada),transthoracic echocardiograms were performed with a 30 MHz transducer(MS 400; Visualsonics). Evaluations included baseline imaging andrepeated 70 days post treatment. Briefly, mice were induced with 3-5%isoflurane and fur removed with depilatory cream (Nair, Church & DwightCo. Inc., Princeton, N.J.), then maintained with 2% isofluranethroughout the two-dimensional and M-mode recording of the LV inparasternal long-axis, short-axis, and four chamber views. Images wereanalyzed post recording using Vevo LAB software (1.7.1, Visualsonics)with a minimum of 3 cardiac cycles measured for each mouse asrecommended by the company. Fractional shortening (FS, %), ejectionfraction (EF, %) and AV peak pressure (mmHg) were calculated usingstandard equations within the software.

Flexivent:

Airway resistance to methacholine challenge (MeCh; Sigma-Aldrich) wasmeasured using the FlexiVent FX System (Scireq, Montreal, QC, Canada).Raw data were fitted into a single—compartment model and resistance datawere calculated. When no significant difference was observed in baselinevalues at 0 mg/ml MeCh among groups, airway resistance was normalizedand presented as normalized resistance((values−baseline)/baseline×100%).

SPO2%:

Using MouseOx (STARR Life Sciences, Oakmont, Pa.) with correspondingmouse thigh sensor, saturated oxygen levels were measured in all mice.Fur was removed using depilatory cream (Nair) over the medial thigh asrecommended for mice with pigmented coats. All recordings were takenover 2-3 minutes while under manual restraint by the same experiencedlab member. Software Version 6.2.1 was used to analyze all recordedmeasures. The highest SPO2% recorded and the corresponding heart (HR)and respiratory rate (RR) were presented.

Statistics:

Statistical analyses were performed using JMP Pro (SAS, Cary, N.C.27513) or SigmaPlot (Systat Software, Inc., San Jose, Calif. 95131)software. If two groups were used in an experiment, then the data wereanalyzed by t-test and if more than two groups were used, then the datawere analyzed by one way ANOVA. Numbers of animals used are indicated ineach figure.

It will be appreciated by a person skilled in the art that the presentinvention is not limited by what has been particularly shown anddescribed hereinabove. Rather, the scope of the invention is defined bythe claims that follow:

What is claimed is:
 1. A method of treating, reducing the severity of,reducing the incidence of, delaying the onset of, or reducing thepathogenesis of myotonic dystrophy, limb-girdle muscular dystrophy,facioscapulhumeral muscular dystrophy, congenital muscular dystrophy,oculopharyngeal muscular dystrophy, distal muscular dystrophy, orEmery-Dreifuss muscular dystrophy in a subject in need thereof,consisting essentially of the step of administering to said subject aselective androgen receptor modulator (SARM) compound represented by thestructure of formula S-XXIII:

or its optical isomer, pharmaceutically acceptable salt, or anycombination thereof.
 2. The method of claim 1, wherein saidadministering comprises administering a pharmaceutical compositioncomprising said compound and/or its optical isomer, pharmaceuticallyacceptable salt, or any combination thereof; and a pharmaceuticallyacceptable carrier.
 3. The method of claim 1, wherein said methodfurther increases the physical function of said subject.
 4. The methodof claim 1, wherein said method further increases the quality of life ofsaid subject.
 5. The method of claim 1, wherein said method furtherdelays onset or improves symptoms of cardiomyopathy and respiratoryfunction.